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Article

Multi-Response Extraction Optimization Based on Anti-Oxidative Activity and Quality Evaluation by Main Indicator Ingredients Coupled with Chemometric Analysis on Thymus quinquecostatus Celak

1
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
2
School of Chinese Pharmacy, Ningxia Medical University, Yinchuan 750004, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Luca Forti
Received: 24 March 2018 / Revised: 10 April 2018 / Accepted: 16 April 2018 / Published: 19 April 2018
(This article belongs to the Collection Recent Advances in Flavors and Fragrances)
Thymus quinquecostatus Celak is a species of thyme in China and it used as condiment and herbal medicine for a long time. To set up the quality evaluation of T. quinquecostatus, the response surface methodology (RSM) based on its 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was introduced to optimize the extraction condition, and the main indicator components were found through an UPLC-LTQ-Orbitrap MSn method. The ethanol concentration, solid-liquid ratio, and extraction time on optimum conditions were 42.32%, 1:17.51, and 1.8 h, respectively. 35 components having 12 phenolic acids and 23 flavonoids were unambiguously or tentatively identified both positive and negative modes to employ for the comprehensive analysis in the optimum anti-oxidative part. A simple, reliable, and sensitive HPLC method was performed for the multi-component quantitative analysis of T. quinquecostatus using six characteristic and principal phenolic acids and flavonoids as reference compounds. Furthermore, the chemometrics methods (principal components analysis (PCA) and hierarchical clustering analysis (HCA)) appraised the growing areas and harvest time of this herb closely relative to the quality-controlled. This study provided full-scale qualitative and quantitative information for the quality evaluation of T. quinquecostatus, which would be a valuable reference for further study and development of this herb and related laid the foundation of further study on its pharmacological efficacy. View Full-Text
Keywords: Thymus quinquecostatus Celak; antioxidative activity; response surface analysis; thyme; quantitative analysis; UPLC-LTQ-Orbitrap MSn; HPLC Thymus quinquecostatus Celak; antioxidative activity; response surface analysis; thyme; quantitative analysis; UPLC-LTQ-Orbitrap MSn; HPLC
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MDPI and ACS Style

Chang, Y.-L.; Shen, M.; Ren, X.-Y.; He, T.; Wang, L.; Fan, S.-S.; Wang, X.-H.; Li, X.; Wang, X.-P.; Chen, X.-Y.; Sui, H.; She, G.-M. Multi-Response Extraction Optimization Based on Anti-Oxidative Activity and Quality Evaluation by Main Indicator Ingredients Coupled with Chemometric Analysis on Thymus quinquecostatus Celak. Molecules 2018, 23, 957. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23040957

AMA Style

Chang Y-L, Shen M, Ren X-Y, He T, Wang L, Fan S-S, Wang X-H, Li X, Wang X-P, Chen X-Y, Sui H, She G-M. Multi-Response Extraction Optimization Based on Anti-Oxidative Activity and Quality Evaluation by Main Indicator Ingredients Coupled with Chemometric Analysis on Thymus quinquecostatus Celak. Molecules. 2018; 23(4):957. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23040957

Chicago/Turabian Style

Chang, Yan-Li, Meng Shen, Xue-Yang Ren, Ting He, Le Wang, Shu-Sheng Fan, Xiu-Huan Wang, Xiao Li, Xiao-Ping Wang, Xiao-Yi Chen, Hong Sui, and Gai-Mei She. 2018. "Multi-Response Extraction Optimization Based on Anti-Oxidative Activity and Quality Evaluation by Main Indicator Ingredients Coupled with Chemometric Analysis on Thymus quinquecostatus Celak" Molecules 23, no. 4: 957. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23040957

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