Next Article in Journal
Application of the Response Surface Methodology to Optimize the Fermentation Parameters for Enhanced Docosahexaenoic Acid (DHA) Production by Thraustochytrium sp. ATCC 26185
Next Article in Special Issue
Effect of Microwave Vacuum Drying on the Drying Characteristics, Color, Microstructure, and Antioxidant Activity of Green Coffee Beans
Previous Article in Journal
The Effects of Chinese Herbal Medicines on the Quorum Sensing-Regulated Virulence in Pseudomonas aeruginosa PAO1
Previous Article in Special Issue
Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics
Article

Antioxidant and Cytoprotective Effects of Kukoamines A and B: Comparison and Positional Isomeric Effect

by 1,2,*,†, 3,4,†, 1,2, 1,2 and 3,4,*
1
School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2
Innovative Research & Development Laboratory of TCM, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
3
School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
4
Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Susana M. Cardoso
Received: 26 March 2018 / Revised: 13 April 2018 / Accepted: 17 April 2018 / Published: 21 April 2018
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products)
In this study, two natural phenolic polyamines, kukoamine A and B, were comparatively investigated for their antioxidant and cytoprotective effects in Fenton-damaged bone marrow-derived mesenchymal stem cells (bmMSCs). When compared with kukoamine B, kukoamine A consistently demonstrated higher IC50 values in PTIO•-scavenging (pH 7.4), Cu2+-reducing, DPPH•-scavenging, •O2-scavenging, and •OH-scavenging assays. However, in the PTIO•-scavenging assay, the IC50 values of each kukoamine varied with pH value. In the Fe2+-chelating assay, kukoamine B presented greater UV-Vis absorption and darker color than kukoamine A. In the HPLC–ESI–MS/MS analysis, kukoamine A with DPPH• produced radical-adduct-formation (RAF) peaks (m/z 922 and 713). The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl (MTT) assay suggested that both kukoamines concentration-dependently increased the viabilities of Fenton-damaged bmMSCs at 56.5–188.4 μM. However, kukoamine A showed lower viability percentages than kukoamine B. In conclusion, the two isomers kukoamine A and B can protect bmMSCs from Fenton-induced damage, possibly through direct or indirect antioxidant pathways, including electron-transfer, proton-transfer, hydrogen atom transfer, RAF, and Fe2+-chelating. Since kukoamine B possesses higher potentials than kukoamine A in these pathways, kukoamine B is thus superior to kukoamine A in terms of cytoprotection. These differences can ultimately be attributed to positional isomeric effects. View Full-Text
Keywords: positional isomeric effect; antioxidant mechanisms; cytoprotective effect; kukoamine A; kukoamine B; phenolic polyamine positional isomeric effect; antioxidant mechanisms; cytoprotective effect; kukoamine A; kukoamine B; phenolic polyamine
Show Figures

Graphical abstract

MDPI and ACS Style

Li, X.; Lin, J.; Chen, B.; Xie, H.; Chen, D. Antioxidant and Cytoprotective Effects of Kukoamines A and B: Comparison and Positional Isomeric Effect. Molecules 2018, 23, 973. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23040973

AMA Style

Li X, Lin J, Chen B, Xie H, Chen D. Antioxidant and Cytoprotective Effects of Kukoamines A and B: Comparison and Positional Isomeric Effect. Molecules. 2018; 23(4):973. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23040973

Chicago/Turabian Style

Li, Xican, Jian Lin, Ban Chen, Hong Xie, and Dongfeng Chen. 2018. "Antioxidant and Cytoprotective Effects of Kukoamines A and B: Comparison and Positional Isomeric Effect" Molecules 23, no. 4: 973. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules23040973

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop