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Article

Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum

1
Qinghai Provincial Key Laboratory of Crop Molecular Breeding, Xining 810008, Qinghai, China
2
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 800010, Qinghai, China
3
Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Received: 31 January 2019 / Revised: 4 March 2019 / Accepted: 5 March 2019 / Published: 7 March 2019
(This article belongs to the Section Chemical Biology)
Red coleoptiles can help crops to cope with adversity and the key genes that are responsible for this trait have previously been isolated from Triticum aestivum, Triticum urartu, and Aegilops tauschii. This report describes the use of transcriptome analysis to determine the candidate gene that controls the trait for white coleoptiles in T. monococcum by screening three cultivars with white coleoptiles and two with red coleoptiles. Fifteen structural genes and two transcription factors that are involved in anthocyanin biosynthesis were identified from the assembled UniGene database through BLAST analysis and their transcript levels were then compared in white and red coleoptiles. The majority of the structural genes reflected lower transcript levels in the white than in the red coleoptiles, which implied that transcription factors related to anthocyanin biosynthesis could be candidate genes. The transcript levels of MYC transcription factor TmMYC-A1 were not significantly different between the white and red coleoptiles and all of the TmMYC-A1s contained complete functional domains. The deduced amino acid sequence of the MYB transcription factor TmMYB-A1 in red coleoptiles was homologous to TuMYB-A1, TaMYB-A1, TaMYB-B1, and TaMYB-D1, which control coleoptile color in corresponding species and contained the complete R2R3 MYB domain and the transactivation domain. TmMYB-a1 lost its two functional domains in white coleoptiles due to a single nucleotide deletion that caused premature termination at 13 bp after the initiation codon. Therefore, TmMYB-A1 is likely to be the candidate gene for the control of the red coleoptile trait, and its loss-of-function mutation leads to the white phenotype in T. monococcum. View Full-Text
Keywords: T. monococcum; coleoptile; anthocyanin biosynthesis; MYB transcription factor T. monococcum; coleoptile; anthocyanin biosynthesis; MYB transcription factor
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MDPI and ACS Style

Cao, D.; Fan, J.; Xi, X.; Zong, Y.; Wang, D.; Zhang, H.; Liu, B. Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum. Molecules 2019, 24, 932. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules24050932

AMA Style

Cao D, Fan J, Xi X, Zong Y, Wang D, Zhang H, Liu B. Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum. Molecules. 2019; 24(5):932. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules24050932

Chicago/Turabian Style

Cao, Dong, Jiequn Fan, Xingyuan Xi, Yuan Zong, Dongxia Wang, Huaigang Zhang, and Baolong Liu. 2019. "Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum" Molecules 24, no. 5: 932. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules24050932

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