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Article

Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation

1
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
2
Integrated Chemical BioPhysics Research, Faculty of Science, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
3
Natural Medicines and Product Research Laboratory, Institute of Biosciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
4
Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang 43300, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editors: Ana Paula Duarte, Ângelo Luís and Eugenia Gallardo
Received: 9 September 2020 / Revised: 19 October 2020 / Accepted: 20 October 2020 / Published: 6 November 2020
(This article belongs to the Special Issue Natural Products: Therapeutic Properties and Beyond)
Scopoletin has previously been reported as a biomarker for the standardization of Paederia foetida twigs. This study is the first report on the determination and quantification of scopoletin using quantitative nuclear magnetic resonance (qNMR) in the different extracts of Paederia foetida twigs. The validated qNMR method showed a good linearity (r2 = 0.9999), limit of detection (LOD) (0.009 mg/mL), and quantification (LOQ) (0.029 mg/mL), together with high stability (relative standard deviation (RSD) = 0.022%), high precision (RSD < 1%), and good recovery (94.08–108.45%). The quantification results of scopoletin concentration in chloroform extract using qNMR and microplate ultraviolet-visible (UV-vis) spectrophotometer was almost comparable. Therefore, the qNMR method is deemed accurate and reliable for quality control of Paederia foetida and other medicinal plants without extensive sample preparation. View Full-Text
Keywords: qNMR; scopoletin; Paederia foetida; method validation; quantification qNMR; scopoletin; Paederia foetida; method validation; quantification
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MDPI and ACS Style

Tan, D.C.; Quek, A.; Kassim, N.K.; Ismail, I.S.; Lee, J.J. Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation. Molecules 2020, 25, 5162. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25215162

AMA Style

Tan DC, Quek A, Kassim NK, Ismail IS, Lee JJ. Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation. Molecules. 2020; 25(21):5162. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25215162

Chicago/Turabian Style

Tan, Dai C., Alexandra Quek, Nur K. Kassim, Intan S. Ismail, and Joanna J. Lee 2020. "Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation" Molecules 25, no. 21: 5162. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules25215162

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