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Review

Nanoparticle-Based Systems for T1-Weighted Magnetic Resonance Imaging Contrast Agents

by
Derong Zhu
1,
Fuyao Liu
2,
Lina Ma
2,*,
Dianjun Liu
2 and
Zhenxin Wang
2,*
1
Department of Medicinal Chemistry and Pharmaceutical Analysis, Guangdong Medical College, Dongwan 523770, Guangdong, China
2
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(5), 10591-10607; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140510591
Submission received: 18 March 2013 / Revised: 9 May 2013 / Accepted: 13 May 2013 / Published: 21 May 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Abstract

:
Because magnetic resonance imaging (MRI) contrast agents play a vital role in diagnosing diseases, demand for new MRI contrast agents, with an enhanced sensitivity and advanced functionalities, is very high. During the past decade, various inorganic nanoparticles have been used as MRI contrast agents due to their unique properties, such as large surface area, easy surface functionalization, excellent contrasting effect, and other size-dependent properties. This review provides an overview of recent progress in the development of nanoparticle-based T1-weighted MRI contrast agents. The chemical synthesis of the nanoparticle-based contrast agents and their potential applications were discussed and summarized. In addition, the recent development in nanoparticle-based multimodal contrast agents including T1-weighted MRI/computed X-ray tomography (CT) and T1-weighted MRI/optical were also described, since nanoparticles may curtail the shortcomings of single mode contrast agents in diagnostic and clinical settings by synergistically incorporating functionality.

Graphical Abstract

1. Introduction

Molecular-diagnostic imaging, a field at the intersection of molecular biology and in vivo imaging, has received considerable attention in the past decades due to its diagnostic and clinical promises [1–10]. Representative imaging platforms/techniques include computed X-ray tomography (CT), optical imaging, magnetic resonance imaging (MRI), positron emission tomography (PET), single-photon-emission computed tomography (SPECT), and ultrasound. These techniques hold promise because they allow real-time visualization of the cellular functions of living organisms and related molecular interactions, and, importantly, they are noninvasive. Of these methods, MRI is currently one of the most powerful diagnostic tools in medical science since it is able to acquire 3-D tomographical information in whole tissue samples, including human soft tissues, and whole animals, at high spatial and temporal resolution [1,5,11–15]. In addition, because MRI images are acquired without the use of ionizing radiation (X-ray/CT) or radiotracers (PET and SPECT), it has been the preferable imaging technique for the heart, brain, and nervous system [16–21]. Although tissue MRI is capable of revealing anatomic details in organs, it is difficult to differentiate normal and diseased cells due to small native relaxation time differences. In this context, imaging sensitivity can be enhanced through the use of MRI contrast agents [12,14,17].
MRI is based on the response of proton spin in the presence of an external magnetic field when triggered with a radio frequency (RF) pulse [11,22,23]. Under the influence of an external magnetic field, protons align in one direction. On application of the RF pulse, aligned protons are perturbed and subsequently relax to their original state. There are two independent relaxation processes: longitudinal (T1) and transverse (T2) relaxation, which are typically used to generate the MR images. Therefore, there are two classes of MRI contrast agents available, (1) T1-weighted contrast agents (e.g., gadolinium- (Gd3+) and manganese- (Mn2+) chelates) are paramagnetic in nature which increase the T1 relaxation time, resulting in bright contrast T1-weighted images; and (2) T2-weighted contrast agents are superparamagnetic materials (e.g., magnetite (Fe3O4) nanoparticles) which reduce T2 relaxation times, giving rise to dark contrast T2-weighted images [11,22,23]. The efficiency of a contrast agent to reduce the T1 or T2 of water protons is referred to as relaxivity and defined by followed equation: 1/T1,2 = 1/T01,2 + r1,2C. Where 1/T1,2 is the observed relaxation rate in the presence of contrast agents, 1/T01,2 is the relaxation rate of pure water, C is the concentration of the contrast agents and r1 and r2 are the longitudinal and transverse relaxivities, respectively [11,21–23].
Recent advances in cross-disciplinary nanoscience and nanotechnology have led to further and rapid developments of novel nanomaterials as MRI contrast agents. Because of their importance in MRI, several reviews on synthesis and applications of nanomaterial-based contrast agents have been published elsewhere [8,9,14,16,17,24–40]. Compared to conventional MRI contrast agents, nanomaterial-based MRI contrast agents offer a number of advantages, (1) biostability and tunable biodistribution can be achieved by surface modification; (2) different degrees of biocompatibility and imaging properties can be adjusted by their chemical composition, shapes and sizes; (3) they can identify the desired target by specific conjugation with biological molecules interactions, such as antibodies, nucleic acids, and peptides; and (4) multimodal imaging can also be achieved using a combination of optical and magnetic properties of nanomaterials. The nanoparticle-based contrast agents were classified and discussed as T1-weighted MRI contrast agents, T2-weighted MRI contrast agents and multimodality imaging contrast agents. This review focuses on recent progresses in nanoparticle-based contrast agents for T1-weighted MRI and T1-weighted MR/optical (fluorescence and X-ray) multimodal imaging. The review highlights the design and fabrication procedures of nanoparticle-based contrast agents and their potential biomedical applications. In addition, we consider the following points: synthetic strategy for improving r1-relaxivity, design of the ligand shell to attain high colloidal stability and biocompatibility, and in vivo MR imaging of cancer. We refer interested readers to other review articles for T2-weighted MRI specific nanoparticulate systems (or in other word nanohybrids) [16,25,26–29,35,36].

2. General Description of T1-Weighted Contrast Agents

T1 relaxation is the process of equilibration of the net magnetization (Mz) after the introduction of an RF pulse. This change of Mz is a consequence of the energy transfer between the proton spin system and the nearby matrix of molecules [11,21,22]. All biological systems are composed of various molecules and organisms which have different proton concentrations and different T1 relaxation times. The presence of paramagnetic ions (e.g., Gd3+ and Mn2+, as shown in Figure 1) near the tissue enhances its relaxation and shortens the T1 relaxation time [11,21–23,29]. Contrast agents with T1-weighted enhancing ability produce bright positive signal intensity in images and increase the conspicuousness of cells, facilitating easy tracking of cells in low-signal tissues [11,21–23]. Among those paramagnetic ions, Gd3+ is the most effective T1-weighted contrast agent for clinical use [17,29,41,42]. It is suggested that more than 10 million MRI studies are performed worldwide using Gd3+-based contrast agents each year [42]. Gd-chelates (e.g., Gd-diethylenetriaminepentaacetic acid (Gd-DTPA) and Gd-N,N′,N″,N‴-tetracarboxymethyl-1,4,7,10-tetraazacyclododecane (Gd-DOTA)) are normally used as T1-weighted MRI contrast agents. Despite their utility, these contrast agents suffer from poor sensitivity and rapid renal clearance, which severely limits the time window for MRI. Considerable efforts have been devoted to incorporate Gd3+ onto or into nanoparticles (e.g., hydrophilic macromolecule nanoparticles including dendrimers, dextran, and other hydrophilic polymers, liposomes, and inorganic nanoparticles (NPs)) [24,29,41–87]. This method can concentrate Gd3+ ion on/in the nanoparticles, resulting in the reduction of the toxicity of Gd3+, and enhancing the T1-weighted MR signal. It can also increase the cellular uptake of Gd3+ ions through size and shape tuning of the vehicle nanoparticles. Furthermore, biological functional groups can be conjugated on the nanoparticles surfaces for studying dynamic biomolecular phenomena through suitable biochemical reaction and signaling. Compared to Gd/Mn chelates, the nanoparticle-based T1-weighted MRI contrast agents have several advantages, including passive targeting properties, the prolonged imaging time, the enhancement of the contrast, and low toxicity [8,14,17,24,26,29,34]. Based on the compositions of nanoparticle-based T1-weighted MRI contrast agents, they can be classified as: (1) Gd-chelate grafted organic/inorganic nanoparticles, (2) gadolinium nanoparticles, (3) manganese-based nanoparticulate systems, and (4) dual (T1- and T2-) weighted MRI contrast agents.

3. Gd-Chelate Grafted Hydrophilic Macromolecule Nanoparticles

Polymeric Gd complexes, based on dextrans, polylysine derivatives, or dendrimers, as well as Gd-chelate grafted latex nanoparticles, liposomes, and micelles have been successfully employed for developing Gd-chelate grafted hydrophilic macromolecule nanoparticle contrast agents [42,50–61]. For instance, branched polyamidoamine (PAMAM) dendrimers are synthetic biocompatible macromolecules, processing multiple free amino groups on the surface. The physical, chemical, and biological characteristics of PAMAM dendrimers make this kind of molecules an ideal template for synthesis of Gd-chelate grafted nanoparticles [51,56,60]. Cheng and coauthors have provided a facile method for the synthesis of nanometer-sized dendrimer nanoclusters (5.8 nm in diameter) [56]. The dendrimer nanoclusters were labeled with Gd by reaction of the amine functional groups with the chelating agent DTPA dianhydride. The Gd-conjugated dendrimer nanoclusters have an r1 relaxivity value of 12.3 mM−1·s−1 per Gd3+ (1.41 T), which is much higher than that of Gd-DTPA (3.9 mM−1·s−1 per Gd3+) under same experimental condition. The ultrasensitive MR detection of various types of cancers may be possible by conjugating appropriate cancer-targeting ligands (e.g., folic acid) with the Gd-conjugated dendrimer nanoclusters. Huang and coauthors have recently reported gadolinium-conjugated PAMAM dendrimer nanoclusters as T1-weighted MRI contrast agents (as shown in Figure 2) [60]. In order to reduce the potential toxicity of PAMAM dendrimer clusters, individual Gd3+ labeled PAMAM dendrimers have been cross-linked to form larger nanoclusters through biodegradable polydisulfide linkages. These biodegradable polydisulfide dendrimer clusters inherit the high relaxivity (T1) of dendrimer clusters (the r1 value is more than 11.7 mM−1·s−1 per Gd3+(1.41 T)) and retain an extended circulation time (circulation half-life > 1.6 h in mice). They are reduced to smaller degradation products while in circulation and undergo efficient renal excretion, reducing the possibility of long-term macromolecular particle retention. Importantly, the size of dendrimer nanoclusters can be easily controlled by adding an excess of maleimide to stop disulfide bond formation at different reaction time points. This strategy has provided a facile method for the synthesis of size-controllable and biodegradable dendrimer nanoclusters for clinical applications. Liu and coauthors designed a novel multifunctional polymeric nanoparticle contrast agent (Anti-VEGF PLA-PEG-PLL-Gd NP) simultaneously modified with Gd-DTPA and anti-vascular endothelial growth factor (VEGF) antibody to deliver Gd-DTPA to the tumor area and achieve the diagnosis of hepatocellular carcinoma (HCC) at an early stage [55]. The Anti-VEGF PLA-PEG-PLL-Gd NPs exhibited high T1 relaxivity [the r1 value is 18.394 mM−1·s−1 per Gd3+ (3.0 T)], good biocompatibility, and excellent selectivity towards tumor cells (as shown in Figure 3). The Anti-VEGF PLA-PEG-PLL-Gd NPs show great potential in the diagnosis of liver tumors at early stages.

4. Gd-Chelate Grafted Inorganic Nanoparticles

Gd-chelate grafted inorganic nanoparticles have recently attracted much attention because they could behave as contrast agents for in vivo multimodality imaging [62–69]. Multimodality imaging enables the providing of more complementary, effective, and accurate information about the physical, anatomical structure, and the physiological function for diagnosis and research. For instance, Gd-chelate (Gd- dithiolated derivatives of diethylenetriaminepentaacetic acid (DTDTPA)) functionalized gold nanoparticles (Au@DTDTPA-Gd nanoparticles) were prepared and applied as contrast agents for both in vivo CT and T1-weighted MRI by Alric and coauthors [62]. The nanoparticles were obtained by encapsulating gold nanoparticle cores within a multilayered organic shell which is composed of Gd-chelates bound to each other through disulfide bonds. Specific targeting of cancer at an early stage can be achieved by the covalent grafting of biotargeting groups on the organic multilayer of the Au@DTDTPA-Gd nanoparticles since each DTDTPA ligand possesses three COOH moieties as anchoring sites. The experimental results also demonstrated that the development of nanoparticles for targeted diagnosis and therapy can, therefore, be envisaged with the Au@DTDTPA-Gd nanoparticles. Xia and coauthors have designed and synthesized a kind of core@shell lanthanide-based nanoparticles, NaLuF4:Yb3+, Tm3+@SiO2-DTPA-Gd nanoparticles (UCNP@SiO2-GdDTPA) with NaLuF4:Yb3+, Tm3+ upconverting nanoparticles (UCNPs) as the core and SiO2 as the shell layer, and the Gd-DTPA as the surface ligand (as shown in Figure 4) [64]. The UCNP@SiO2-GdDTPA can be employed as a contrast agent for near-infrared to near-infrared (NIR-to-NIR) upconversion luminescence (UCL), CT and T1-weighted MR trimodality in vivo imaging. Gd3+ binding on the surface of nanoparticles makes the core@shell nanoparticles show high r1 relaxivity [6.35 mM−1·s−1 (0.5 T)] and suitable for T1-weighted MRI. The UCNP@SiO2-GdDTPA have been applied in the trimodal NIR-to-NIR UCL, CT, and T1-weighted MR molecular imaging for small animals, both in vivo and in vitro. Wen and coauthors have designed and synthesized multifunctional Gd-loaded Au DENPs (Gd–Au DENPs) [65]. In this case, amine-terminated generation 5 PAMAM dendrimers (G5·NH2), modified with Gd-chelator and polyethylene glycol (PEG) monomethyl ether, were used as templates to synthesize gold nanoparticles (Au NPs) within the dendrimer interior (Au DENPs) (as shown in Figure 5). The formed G5·NH2 with Gd-chelators were complexed with Gd3+, followed by complete acetylation of the remaining dendrimer terminal amines. The as-prepared Gd–Au DENPs are water soluble, colloidally stable, and non-cytotoxic in the given concentration range. The Gd–Au DENPs have an r1 relaxivity of 1.05 mm−1·s−1 per Gd3+ (3.0 T). The experimental result demonstrates that the Gd–Au DENPs enable to be used as dual mode (CT/MRI) contrast agents for in vivo imaging of some major organs of rats and mice. Taking into consideration of the unique structural characteristics of the dendrimers that can be further functionalized with various targeting ligands, it is expected that the developed Gd–Au DENPs may be used as a multifunctional nanoplatform for targeted CT/MR dual mode imaging of various biological systems, especially for diagnosis of cancer at early stage with high accuracy and high sensitivity.

5. Gadolinium Nanoparticles

Recently, inorganic nanoparticles containing Gd3+ [e.g., gadolinium oxide (Gd2O3), gadolinium fuoride (GdF3) and gadolinium phosphate (GdPO4)] have been investigated as T1-weighted MRI contrast agents [70–87]. Compare to Gd-chelate grafted nanoparticles, these nanoparticles have several distinct advantages: (1) ease of synthesis and functionalization, (2) the ability to carry large payloads of active magnetic centers, (3) decreased tumbling rates, which lead to increased relaxivity values. For example, most of these nanoparticles showed larger r1 values than those of the Gd-chelates, depending on their diameters. Ultrasmall Gd2O3 nanoparticles, with an average diameter of 1 nm, have been prepared by Park and coauthors [70]. Surface Gd3+ ions in the Gd2O3 nanoparticles cooperatively induce the longitudinal relaxation of the water proton. Thus, the ultrasmall Gd2O3 nanoparticles provided the r1 of 9.9 mm−1·s−1 (1.5 T), which is much larger than those of Gd-chelates. After injection with d-glucuronic acid coated ultrasmall Gd2O3 nanoparticles, high contrast in vivo T1-weighted MR images of the brain tumor of a rat were observed. Very recently, Liang and coauthors have developed a simple, cost effective, and easy to scale up strategy for synthesized poly-(acrylic acid)-coated, ultrasmall paramagnetic gadolinium hydrated carbonate nanoparticle (GHC-1) with small size (~2 nm in diameter) [78]. The GHC-1 exhibits a large longitudinal relaxivity of 34.8 mm−1·s−1 (0.55 T) while maintaining an r2/r1 ratio as low as 1.17, making it effective as a T1-weighted MRI contrast agent. More importantly, the presence of carboxylic acid groups on the external surface of the nanoparticles is thought to be useful for conjugation of targeting molecules, such as antibodies and peptides, to the nanoparticles for specific tumor imaging.
Rare earth (RE) ions doped UCNPs have been of considerable interest in recent years due to their applications in biomedical imaging, solar cells, lasers, lighting, and display technologies [79–87]. Among upconverting nanomaterials, the Gd3+ contained UCNPs have shown excellent UCL, unique MR and strong X-ray attenuation. Therefore, the Gd3+ contained UCNPs can be employed as high performance contrast agents for UCL, MR and CT imaging. Anti-EGFR monoclonal antibody (mAb) conjugated NaGdF4 nanocrystals (NaGdF4-PEG-mAb) with narrow particle size distributions were synthesized by Hou and coauthors [86]. The experimental results revealed that the NaGdF4-PEG-mAb probes possessed satisfying tumor-specific targeting ability and strong MRI contrast enhancement effects. Liu and coauthors successfully synthesized a high-quality PEGylated Gd2O3:Yb3+, Er3+ nanorods (PEG-UCNPs) for in vivo UCL, T1-weighted MR, and CT multimodality imaging [87]. As an alternative to lanthanide-doped fluoride, the oxide-based nanoprobes feature superior properties, such as easy decomposition inside macrophage cells after reticuloendothelial system (RES) uptake and nearly total excretion from the mouse body.

6. Manganese-Based Nanoparticulate Systems

Mn2+ is another cation could be used as the MRI contrast agent since it has five unpaired electrons with long electronic relaxation time. However, it is very difficult to design and synthesize highly stable Mn2+ complexes with high sensitivities for clinical applications. This drawback can be overcome by building manganese-based nanoparticulate systems, such as MnO, Mn3O4, Mn3O4@SiO2, MnO@mesoporous SiO2, and even hollow MnO nanoparticles [88–94]. Many approaches have been developed to synthesize manganese oxide (MnO or Mn3O4) nanoparticles. One common route is to heat up Mn-oleate in a high boiling point solvent (e.g., 1-octadecene) to induce nucleation and particle growth [93]. This method allows the preparation of nanoparticles with accurate size control. The enhancement of the accessibility of the manganese paramagnetic centers to water molecules is the key issue to be addressed to design highly efficient manganese-based MRI contrast agents [88–94]. Kim and coauthors report on a novel design of MnO nanoparticles that have a ‘hollow’ MnO core structure and a coating consisting of mesoporous silica (HMnO@mSiO2) (as shown in Figure 6) [89]. These HMnO@mSiO2 nanoparticles showed a significantly higher r1 relaxivity (0.99 mM−1·s−1 (11.7 T)) over other existing manganese oxide nanoparticle-based contrast agents. The porous coating, which enables water exchange across the shell, combined with the large surface area-to-volume ratio resulting from the novel structure increases water accessibility to the manganese core and consequently provides enhanced T1-weighted contrast. Both in vivo and in vitro experimental results demonstrated that the HMnO@mSiO2 nanoparticles have a great potential application of T1-weighted MRI cell tracking. Chen and coauthors have reported on a synthetic strategy of chemical oxidation/reduction reaction in-situ in mesopores, followed by hydrogen reduction, for the fabrication of non-toxic manganese oxide/mesoporous silica nanoparticle (MSN)-based T1-weighted MRI contrast agents with highly comparable imaging performance to commercial Gd-based agents (as shown in Figure 7) [90]. This strategy involves a “soft-templating” process to prepare MSNs, in-situ reduction of MnO4 − by the “soft templates” in mesopores and heat treatment under reducing atmosphere, to disperse MnO nanoparticles within mesopores. This material system has two prominent advantages: (1) highly dispersed MnO nanoparticles in the penetrating mesopore system ensures the high water-accessibility to manganese paramagnetic centers, and (2) large surface area and pore volume of mesopores make take up a large amount of therapeutic agents within the pore system possible.

7. Dual (T1- and T2-) Weighted MRI Contrast Agents

Both T1-weighted and T2-weighted contrast agents have their own advantages and disadvantages. For example, Gd-based T1-weighted MRI contrast agents have excellent enhancement but they have risks of biological toxicity [95]. In terms of low toxicity, magnetic iron oxide nanoparticle-based T2-weighted MRI contrast agents have been proven to be one of the most promising contrast agents for clinical use because they are naturally found in human body. However, their negative contrast is often confused with a low-level MR signal arising from adjacent tissues such as bone or vasculature since the magnetite nanoparticles represent dark areas in MR images [96]. Therefore, it is highly desirable to prepare robust dual MRI contrast agents for overcoming the disadvantages of single modality contrast agents. The simultaneous use of positive and negative MR imaging that employs the same contrast agents will significantly improve detection accuracy [97–100]. Monodispersed water-soluble and biocompatible ultrasmall magnetic iron oxide nanoparticles (UMIONs, 3.3 ± 0.5 nm in diameter) generated from a high temperature coprecipitation route are successfully used as efficient positive and negative dual contrast agents of MRI by Li and coauthors [99]. The experimental results demonstrate the great potential of the UMIONs in dual contrast agents, especially as an alternative to Gd-based T1-weighted contrast agents, which have risks of inducing side effects in patients.

8. Conclusions and Outlook

Over last decade, extensive research has been conducted to develop nanoparticle-based T1-weighted contrast agents to overcome the drawbacks of clinic Gd chelate-based T1-weighted contrast agents and iron oxide nanoparticle-based negative T2-weighted contrast agents. These new MRI contrast agents including Gd chelate-grafted nanoparticle and Gd/Mn contained inorganic nanoparticles are expected to provide exquisite sensitivity and specificity in disease diagnosis and tracking biological processes such as cancer development and metastasis, cell evolution, and cell-to-cell interactions. In particular, MRI of nanoparticle-based T1-weighted contrast agents in the cardiovascular system has the potential to become a powerful technology in both basic science as well as clinical settings. In addition, multimodal imaging or simultaneous imaging and therapy can be achieved through the combinations of various nanomaterials.
Although many of these promises have been realized in the in vitro testing or preliminary animal studies, significant obstacles still exist in translating these results into clinical diagnosis. For instance, it is difficult to precisely trace the in vivo behavior of these nanoparticle-based T1-weighted contrast agents, such as accumulation, degradation, and clearance. To overcome these obstacles, several fundamental issues will have to be clearly addressed, including synthesis scale, human compatibility, long-term stability, targeting efficiency, and pharmacokinetics. Interdisciplinary collaborative research is needed to both optimize methods for synthesis of highly reproducible nanoparticle-based T1-weighted contrast agents and to gain a better understanding of correlation between the basic physicochemical properties of these nanoparticles and their in vivo biological behaviors. They should be surmountable in the near future because these challenges are not unique to MRI contrast agents.

Acknowledgements

The authors would like to thank National Basic Research Program of China (No. 2011CB935800) for financial support.

Conflict of Interest

The authors declare no conflict of interest.

References

  1. Benaron, D.A. The future of cancer imaging. Cancer Metast. Rev 2002, 21, 45–78. [Google Scholar]
  2. Townsend, D.W.; Beyer, T.; Blodgett, T.M. PET/CT scanners: A hardware approach to image fusion. Semin. Nucl. Med 2003, 33, 193–204. [Google Scholar]
  3. Massoud, T.F.; Gamrbhi, S.S. Molecular imaging in living subjects: Seeing fundamental biological processes in a new light. Genes Dev 2003, 17, 545–580. [Google Scholar]
  4. Brenner, D.J.; Hall, E.J. Computed tomography—An increasing source of radiation exposure. N. Engl. J. Med 2007, 357, 2277–2284. [Google Scholar]
  5. Logothetis, N.K. What we can do and what we cannot do with fMRI. Nature 2008, 453, 869–878. [Google Scholar]
  6. Rabin, J.M.; Perez, J.; Grimm, G.; Wojtkiewicz, G.; Weissleder, R. An X-ray computed tomography imaging agent based on long-circulating bismuth sulphide nanoparticles. Nat. Mater 2006, 5, 118–122. [Google Scholar]
  7. Kobayashi, H.; Ogawa, M.; Alford, R.; Choyke, P.L.; Urano, Y. New strategies for fluorescent probe design in medical diagnostic imaging. Chem. Rev 2010, 110, 2620–2640. [Google Scholar]
  8. Louie, A. Multimodality imaging probes: Design and challenges. Chem. Rev 2010, 110, 3146–3195. [Google Scholar]
  9. Terreno, E.; Castelli, D.D.; Aime, A.S.V. Challenges for molecular magnetic resonance imaging. Chem. Rev 2010, 110, 3019–3042. [Google Scholar]
  10. Zhou, J.; Liu, Z.; Li, F.Y. Upconversion nanophosphors for small-animal imaging. Chem. Soc. Rev 2012, 41, 1323–1349. [Google Scholar]
  11. Mitchell, D.G. MRI Principles; W. B. Saunders Company: Philadelphia, PA, USA, 1999. [Google Scholar]
  12. Artemov, D.; Bhujwalla, Z.M.; Bulte, J.W. Magnetic resonance imaging of cell surface receptors using targeted contrast agents. Curr. Pharm. Biotechnol 2004, 5, 485–494. [Google Scholar]
  13. Blamire, A.M. The technology of MRI—The next 10 years? Br. J. Radiol 2008, 81, 601–617. [Google Scholar]
  14. Xu, W.; Kattel, K.; Park, J.Y.; Chang, Y.T.; Kim, J.; Lee, G.H. Paramagnetic nanoparticle T1 and T2 MRI contrast agents. Phys. Chem. Chem. Phys 2012, 14, 12687–12700. [Google Scholar]
  15. Delikatny, E.J.; Poptani, H. MR techniques for in vivo molecular and cellular imaging. Radiol. Clin. North Am 2005, 43, 205–220. [Google Scholar]
  16. Weissleder, R. Molecular imaging in cancer. Science 2006, 312, 1168–1171. [Google Scholar]
  17. De, M.; Chou, S.S.; Joshi, H.M.; Dravid, V.P. Hybrid magnetic nanostructures (MNS) for magnetic resonance imaging applications. Adv. Drug Deliver. Rev 2011, 63, 1282–1299. [Google Scholar]
  18. Heeger, D.J.; Ress, D. What does fMRI tell us about neuronal activity? Nat. Rev. Neurosci 2002, 3, 142–151. [Google Scholar]
  19. Sosnovik, D.E.; Nahrendorf, M.; Weissleder, R. Molecular magnetic resonance imaging in cardiovascular medicine. Circulation 2007, 115, 2076–2086. [Google Scholar]
  20. Francois, C.J.; Schiebler, M.L.; Reeder, S.B. Cardiac MRI evaluation of nonischemic cardiomyopathies. J. Magn. Reson. Imaging 2010, 31, 518–530. [Google Scholar]
  21. Seevinck, P.R.; Deddens, L.H.; Dijkhuizen, R.M. Magnetic resonance imaging of brain angiogenesis after stroke. Angiogenesis 2010, 13, 101–111. [Google Scholar]
  22. Berry, E.; Bulpitt, A. Fundamentals of MRI; CRC Press: Boca Raton, FL, USA, 2009; p. 298. [Google Scholar]
  23. Westbrook, C. MRI in Practice, 3rd ed; Wiley-Blackwell: Hoboken, NJ, USA, 2005; p. 424. [Google Scholar]
  24. Caravan, P. Strategies for increasing the sensitivity of gadolinium based MRI contrast agents. Chem. Soc. Rev 2006, 35, 512–523. [Google Scholar]
  25. Corot, C.; Robert, P.; Idée, J.-M.; Port, M. Recent advances in iron oxide nanocrystal technology for medical imaging. Adv. Drug Deliver. Rev 2006, 58, 1471–1504. [Google Scholar]
  26. Hu, F.; Zhao, Y.S. Inorganic nanoparticle-based T1 and T1/T2 magnetic resonance contrast probes. Nanoscale 2012, 4, 6235–6243. [Google Scholar]
  27. Stephen, Z.R.; Kievit, F.M.; Zhang, M. Magnetic nanoparticles for medical MR imaging. Mater. Today 2011, 14, 330–338. [Google Scholar]
  28. Frey, N.A.; Peng, S.; Cheng, K.; Sun, S. Magnetic nanoparticles: Synthesis, functionalization, and applications in bioimaging and magnetic energy storage. Chem. Soc. Rev 2009, 38, 2532–2542. [Google Scholar]
  29. Na, H.B.; Song, I.C.; Hyeon, T. Inorganic nanoparticles for MRI contrast agents. Adv. Mater 2009, 21, 2133–2148. [Google Scholar]
  30. Shan, L.; Chopra, A.; Leung, K.; Eckelman, W.C.; Menkens, A.E. Characterization of nanoparticle-based contrast agents for molecular magnetic resonance imaging. J. Nanopart. Res 2012, 14, 1122. [Google Scholar]
  31. Sosnovik, D.E.; Nahrendorf, M.; Weissleder, R. Magnetic nanoparticles for MR imaging: Agents, techniques and cardiovascular applications. Basic Res. Cardiol 2008, 103, 122–130. [Google Scholar]
  32. Jennings, L.E.; Long, N.J. ‘Two is better than one’—Probes for dual-modalitymolecular imaging. Chem. Comm. 2009, 3511–3524. [Google Scholar]
  33. Major, J.L.; Meade, T.J. Bioresponsive, cell-penetrating, and multimeric MR contrast agents. Acc. Chem. Res 2009, 42, 893–903. [Google Scholar]
  34. Hu, F.; Joshi, H.M.; Dravid, V.P.; Meade, T.J. High-performance nanostructured MR contrast probes. Nanoscale 2010, 2, 1884–1891. [Google Scholar]
  35. Liu, F.; Laurent, S.; Fattahi, H.; Vander Elst, L.; Muller, R.N. Superparamagnetic nanosystems based on iron oxide nanoparticles for biomedical imaging. Nanomedicine 2011, 6, 519–528. [Google Scholar]
  36. Jun, Y.-W.; Lee, J.-H.; Cheon, J. Chemical design of nanoparticle probes for high-performance magnetic resonance imaging. Angew. Chem. Int. Ed 2008, 47, 5122–5135. [Google Scholar]
  37. Taylor, A.; Wilson, K.M.; Murray, P.; Fernig, D.G.; Levy, R. Long-term tracking of cells using inorganic nanoparticles as contrast agents: Are we there yet? Chem. Soc. Rev 2012, 41, 2707–2717. [Google Scholar]
  38. Sailor, M.J.; Park, J.-H. Hybrid nanoparticles for detection and treatment of cancer. Adv. Mater 2012, 24, 3779–3802. [Google Scholar]
  39. Goodwill, P.W.; Saritas, E.U.; Croft, L.R.; Kim, T.N.; Krishnan, K.M.; Schaffer, D.V.; Conolly, S.M. X-Space MPI: Magnetic nanoparticles for safe medical imaging. Adv. Mater 2012, 24, 3870–3877. [Google Scholar]
  40. Fang, C.; Zhang, M. Multifunctional magnetic nanoparticles for medical imaging applications. J. Mater. Chem 2009, 19, 6258–6266. [Google Scholar]
  41. Caravan, P.; Ellison, J.J.; McMurry, T.J.; Lauffer, R.B. Gadolinium(III) chelates as MRI contrast agents: Structure, dynamics, and applications. Chem. Rev 1999, 99, 2293–2352. [Google Scholar]
  42. Ratzinger, G.; Agrawal, P.; Körner, W.; Lonkai, J.; Sanders, H.M.H.F.; Terreno, E.; Wirth, M.; Strijkers, G.J.; Nicolay, K.; Gabor, F. Surface modification of PLGA nanospheres with Gd-DTPA and Gd-DOTA for high-relaxivity MRI contrast agents. Biomaterials 2010, 31, 8716–8723. [Google Scholar]
  43. Flacke, S.; Fischer, S.; Scott, M.J.; Fuhrhop, R.J.; Allen, J.S.; McLean, M.; Winter, P.; Sicard, G.A.; Gaffney, P.J.; Wickline, S.A.; et al. Novel MRI contrast agent for molecular imaging of fibrin: Implications for detecting vulnerable plaques. Circulation 2001, 104, 1280–1285. [Google Scholar]
  44. Winter, P.M.; Morawski, A.M.; Caruthers, S.D.; Fuhrhop, R.W.; Zhang, H.; Williams, T.A.; Allen, J.S.; Lacy, E.K.; Robertson, J.D.; Lanza, G.M.; et al. Molecular imaging of angiogenesis in early-stage atherosclerosis with αvβ3-integrin-targeted nanoparticles. Circulation 2003, 108, 2270–2274. [Google Scholar]
  45. Rieter, W.J.; Kim, J.S.; Taylor, K.M.L.; An, H.; Lin, W.; Tarrant, T.; Lin, W. Hybrid silica nanoparticles for multimodal imaging. Angew. Chem. Int. Ed 2007, 46, 3680–3682. [Google Scholar]
  46. Rieter, W.J.; Taylor, K.M.L.; An, H.; Lin, W.; Lin, W. Nanoscale metal-organic frameworks as potential multimodal contrast enhancing agents. J. Am. Chem. Soc 2006, 128, 9024–9025. [Google Scholar]
  47. Taylor, K.M.L.; Kim, J.S.; Rieter, W.J.; An, H.; Lin, W.; Lin, W. Mesoporous silica nanopheres as highly efficient MRI contrast agents. J. Am. Chem. Soc 2008, 130, 2154–2155. [Google Scholar]
  48. Richard, C.; Doan, B.-T.; Beloeil, J.-C.; Bessodes, M.; Tóth, É.; Scherman, D. Noncovalent functionalization of carbon nanotubes with amphiphilic Gd3+ chelates: Toward powerful T1 and T2 MRI contrast agents. Nano Lett 2008, 8, 232–236. [Google Scholar]
  49. Sitharaman, B.; Kissell, K.R.; Hartman, K.B.; Tran, L.A.; Baikalov, A.; Rusakova, I.; Sun, Y.; Khant, H.A.; Ludtke, S.J.; Chiu, W.; et al. Superparamagnetic gadonanotubes are high-performance MRI contrast agents. Chem. Commun 2005, 3915–3917. [Google Scholar]
  50. Lee, G.H.; Chang, Y.; Kim, T.-J. Blood-Pool and targeting MRI contrast agents: From Gd-chelates to Gd-nanoparticles. Eur. J. Inorg. Chem. 2012, 1924–1933. [Google Scholar]
  51. Swanson, S.D.; Kukowska-Latallo, J.F.; Patri, A.K.; Chen, C.; Ge, S.; Cao, Z.; Kotlyar, A.; East, A.T.; Baker, J.R. Targeted gadolinium-loaded dendrimer nanoparticles for tumor-specific magnetic resonance contrast enhancement. Int. J. Nanomed 2008, 3, 201–210. [Google Scholar]
  52. Rowe, M.D.; Thamm, D.H.; Kraft, S.L.; Boyes, S.G. Polymer-modified gadolinium metal-organic framework nanoparticles used as multifunctional nanomedicines for the targeted imaging and treatment of cancer. Biomacromolecules 2009, 10, 983–993. [Google Scholar]
  53. Yim, H.; Yang, S.-G.; Jeon, Y.S.; Park, I.S.; Kim, M.; Lee, D.H.; Bae, Y.H.; Na, K. The performance of gadolinium diethylene triamine pentaacetate-pullulan hepatocyte-specific T1 contrast agent for MRI. Biomaterials 2011, 32, 5187–5194. [Google Scholar]
  54. Chen, K.-J.; Wolahan, S.M.; Wang, H.; Hsu, C.-H.; Chang, H.-W.; Durazo, A.; Hwang, L.-P.; Garcia, M.A.; Jiang, Z.K.; Wu, L.; et al. A small MRI contrast agent library of gadolinium(III)-encapsulated supramolecular nanoparticles for improved relaxivity and sensitivity. Biomaterials 2011, 32, 2160–2165. [Google Scholar]
  55. Liu, Y.J.; Chen, Z.J.; Liu, C.X.; Yu, D.X.; Lu, Z.J.; Zhang, N. Gadolinium-loaded polymeric nanoparticles modified with anti-VEGF as multifunctional MRI contrast agents for the diagnosis of liver cancer. Biomaterials 2011, 32, 5167–5176. [Google Scholar]
  56. Cheng, Z.L.; Thorek, D.L.J.; Tsourkas, A. Gadolinium-conjugated dendrimer nanoclusters as a tumor-targeted T1 magnetic resonance imaging contrast agent. Angew. Chem. Int. Ed 2010, 49, 346–350. [Google Scholar]
  57. Kumar, R.; Ohulchanskyy, T.Y.; Turowski, S.G.; Thompson, M.E.; Seshadri, M.; Prasad, P.N. Combined magnetic resonance and optical imaging of head and neck tumor xenografts using Gadolinium-labelled phosphorescent polymeric nanomicelles. Head Neck Oncol 2010, 2, 35. [Google Scholar]
  58. Bruckman, M.A.; Hern, S.; Jiang, K.; Flask, C.A.; Yu, X.; Steinmetz, N.F. Tobacco mosaic virus rods and spheres as supramolecular high-relaxivity MRI contrast agents. J. Mater. Chem. B 2013, 1. [Google Scholar] [CrossRef]
  59. Rowe, T.M.D.; Chang, C.-C.; Thamm, D.H.; Kraft, S.L.; Harmon, J.F.; Vogt, A.P.; Sumerlin, B.S.; Boyes, S.G. Tuning the magnetic resonance imaging properties of positive contrast agent nanoparticles by surface modification with RAFT polymers. Langmuir 2009, 25, 9487–9499. [Google Scholar]
  60. Huang, C.-H.; Nwe, K.; Zaki, A.A.; Brechbiel, M.W.; Tsourkas, A. Biodegradable polydisulfide dendrimer nanoclusters as MRI contrast agents. ACS Nano 2012, 6, 9416–9424. [Google Scholar]
  61. Hou, S.J.; Tong, S.; Zhou, J.; Bao, C. Block copolymer-based gadolinium nanoparticles as MRI contrast agents with high T1 relaxivity. Nanomedicine 2012, 7, 211–218. [Google Scholar]
  62. Alric, C.; Taleb, J.; Duc, G.L.; Mandon, C.; Billotey, C.; Meur-Herland, A.L.; Brochard, T.; Vocanson, F.; Janier, M.; Perriat, P.; et al. Gadolinium chelate coated gold nanoparticles as contrast agents for both X-ray computed tomography and magnetic resonance imaging. J. Am. Chem. Soc 2008, 130, 5908–5915. [Google Scholar]
  63. Moriggi, L.; Cannizzo, C.; Dumas, E.; Mayer, C.R.; Ulianov, A.; Helm, L. Gold nanoparticles functionalized with gadolinium chelates as high-relaxivity MRI contrast agents. J. Am. Chem. Soc 2009, 131, 10828–10829. [Google Scholar]
  64. Xia, A.; Chen, M.; Gao, Y.; Wu, D.M.; Feng, W.; Li, F.Y. Gd3+ complex-modified NaLuF4-based upconversion nanophosphors for trimodality imaging of NIR-to-NIR upconversion luminescence, X-Ray computed tomography and magnetic resonance. Biomaterials 2012, 33, 5394–5405. [Google Scholar]
  65. Wen, S.; Li, K.; Cai, H.; Chen, Q.; Shen, M.; Huang, Y.; Peng, C.; Hou, W.; Zhu, M.; Zhang, G.; et al. Multifunctional dendrimer-entrapped gold nanoparticles for dual mode CT/MR imaging applications. Biomaterials 2013, 34, 1570–1580. [Google Scholar]
  66. Duncan, A.K.; Klemm, P.J.; Raymond, K.N.; Landry, C.C. Silica microparticles as a solid support for gadolinium phosphonate magnetic resonance imaging contrast agents. J. Am. Chem. Soc 2012, 134, 8046–8049. [Google Scholar]
  67. Sharma, P.; Bengtsson, N.E.; Walter, G.A.; Sohn, H.-B.; Zhou, G.; Iwakuma, N.; Zeng, H.; Grobmyer, S.R.; Scott, E.W.; Moudgil, B.M. Gadolinium-doped silica nanoparticles encapsulating indocyanine green for near infrared and magnetic resonance imaging. Small 2012, 8, 2856–2868. [Google Scholar]
  68. Lin, W.-I.; Lin, C.-Y.; Lin, Y.-S.; Wu, S.-H.; Huang, Y.-R.; Hung, Y.; Chang, C.; Mou, C.-Y. High payload Gd(III) encapsulated in hollow silica nanospheres for high resolution magnetic resonance imaging. J. Mater. Chem. B 2013, 1, 639–645. [Google Scholar]
  69. Hifumi, H.; Yamaoka, S.; Tanimoto, A.; Citterio, D.; Suzuki, K. Gadolinium-based hybrid nanoparticles as a positive MR contrast agent. J. Am. Chem. Soc 2006, 128, 15090–15091. [Google Scholar]
  70. Park, J.Y.; Baek, M.J.; Choi, E.S.; Woo, S.; Kim, J.H.; Kim, T.J.; Jung, J.C.; Chae, K.S.; Chang, Y.; Lee, G.H. Paramagnetic ultrasmall gadolinium oxide nanoparticles as advanced T1 MRI contrast agent: account for large longitudinal relaxivity, optimal particle diameter, and in vivo T1 MR images. ACS Nano 2009, 3, 3663–3669. [Google Scholar]
  71. Tseng, C.-L.; Shih, I.-L.; Stobinski, L.; Lin, F.-H. Gadolinium hexanedione nanoparticles for stem cell labeling and tracking via magnetic resonance imaging. Biomaterials 2010, 31, 5427–5435. [Google Scholar]
  72. Fillmore, H.L.; Shultz, M.D.; Henderson, S.C.; Cooper, P.; Broaddus, W.C.; Chen, Z.J.; Shu, C.-Y.; Zhang, J.; Ge, J.; Dorn, H.C.; et al. Conjugation of functionalized gadolinium metallofullerenes with IL-13 peptides for targeting and imaging glial tumors. Nanomedicine 2011, 6, 449–458. [Google Scholar]
  73. Faucher, L.; Guay-Begin, A.-A.; Lagueux, J.; Cote, M.-F.; Petitclerc, E.; Fortin, M.-A. Ultra-small gadolinium oxide nanoparticles to image brain cancer cells in vivo with MRI. Contrast Media Mol. Imaging 2011, 6, 209–218. [Google Scholar]
  74. Rahman, A.T.M.A.; Majewski, P.; Vasilev, K. Gd2O3 nanoparticles: Size-dependent nuclear magnetic resonance. Contrast Media Mol. Imaging 2013, 8, 92–95. [Google Scholar]
  75. Kryza, D.; Taleb, J.; Janier, M.; Marmuse, L.; Miladi, I.; Bonazza, P.; Louis, C.; Perriat, P.; Roux, S.; Tillement, O.; et al. Biodistribution study of nanometric hybrid gadolinium oxide particles as a multimodal SPECT/MR/Optical imaging and theragnostic agent. Bioconjugate Chem 2011, 22, 1145–1152. [Google Scholar]
  76. Faucher, L.; Gossuin, Y.; Hocq, A.; Fortin, M.A. Impact of agglomeration on the relaxometric properties of paramagnetic ultra-small gadolinium oxide nanoparticles. Nanotechnology 2011, 22, 295103. [Google Scholar]
  77. Azizian, G.; Riyahi-Alam, N.; Haghgoo, S.; Moghimi, H.R.; Zohdiaghdam, R.; Rafiei, B.; Gorji, E. Synthesis route and three different core-shell impacts on magnetic characterization of gadolinium oxide-based nanoparticles as new contrast agents for molecular magnetic resonance imaging. Nanoscale Res. Lett 2012, 7, 549–555. [Google Scholar]
  78. Liang, G.; Cao, L.; Chen, H.; Zhang, Z.; Zhang, S.; Yu, S.; Shen, X.; Kong, J. Ultrasmall gadolinium hydrated carbonate nanoparticle: An advanced T1 MRI contrast agent with large longitudinal relaxivity. J. Mater. Chem. B 2013, 1, 629–638. [Google Scholar]
  79. Hinklin, T.R.; Rand, S.C.; Laine, R.M. Transparent, polycrystalline upconverting nanoceramics: Towards 3-D displays. Adv. Mater 2008, 20, 1270–1273. [Google Scholar]
  80. He, M.; Huang, P.; Zhang, C.L.; Hu, H.Y.; Bao, C.C.; Gao, G. Dual phase-controlled synthesis of uniform lanthanide-doped NaGdF4 upconversion nanocrystals via an OA/ionic liquid two-phase system for in vivo dual-modality imaging. Adv. Funct. Mater 2011, 21, 4470–4477. [Google Scholar]
  81. Das, G.K.; Zhang, Y.; D’Silva, L.; Padmanabhan, P.; Heng, B.C.; Loo, J.S.C.; Selvan, S.T.; Bhakoo, K.K.; Tan, T.T.Y. Single-phase Dy2O3:Tb3+ nanocrystals as dual-modal contrast agent for high field magnetic resonance and optical imaging. Chem. Mater 2011, 21, 2439–2446. [Google Scholar]
  82. Zeng, S.J.; Tsang, M.-K.; Chan, C.-F.; Wong, K.-L.; Hao, J.H. PEG modified BaGdF5:Yb/Er nanoprobes for multi-modal upconversio fluorescent, in vivo X-ray computed tomography and biomagnetic imaging. Biomaterials 2012, 33, 9232–9238. [Google Scholar]
  83. Wang, Y.F.; Sun, L.D.; Xiao, J.W.; Feng, W.; Zhou, J.C.; Shen, J. Rare-Earth nanoparticles with enhanced upconversion emission and suppressed rare-earth-ion leakage. Chem. Eur. J 2012, 18, 5558–5564. [Google Scholar]
  84. Jung, J.; Kim, M.A.; Cho, J.-H.; Lee, S.J.; Yang, I.; Cho, J.; Kim, S.K.; Lee, C.; Park, J.K. Europium-doped gadolinium sulfide nanoparticles as a dual-mode imaging agent for T1-weighted MR and photoluminescence imaging. Biomaterials 2012, 33, 5865–5874. [Google Scholar]
  85. Wu, Y.; Xu, X.; Tang, Q.; Li, Y. A new type of silica-coated Gd2(CO3)3:Tb nanoparticle as a bifunctional agent for magnetic resonance imaging and fluorescent imaging. Nanotechnology 2012, 23, 205103. [Google Scholar]
  86. Hou, Y.; Qiao, R.; Fang, F.; Wang, X.; Dong, C.; Liu, K.; Liu, C.; Liu, Z.; Lei, H.; Wang, F.; et al. NaGdF4 nanoparticle-based molecular probes for magnetic resonance imaging of intraperitoneal tumor xenografts in vivo. ACS Nano 2013, 7, 330–338. [Google Scholar]
  87. Liu, Z.; Pu, F.; Huang, S.; Yuan, Q.; Ren, J.; Qu, X. Long-circulating Gd2O3:Yb3+, Er3+ up-conversion nanoprobes as high-performance contrast agents for multi-modality imaging. Biomaterials 2013, 34, 1712–1721. [Google Scholar]
  88. Silva, A.C.; Lee, J.H.; Aoki, I.; Koretsky, A.P. Manganese-enhanced magnetic resonance imaging (MEMRI): Methodological and practical considerations. NMR Biomed 2004, 17, 532–543. [Google Scholar]
  89. Kim, T.; Momin, E.; Choi, J.; Yuan, K.; Zaidi, H.; Kim, J.; Park, M.; Lee, N.; McMahon, M.T.; Quinones-Hinojosa, A.; et al. Mesoporous silica-coated hollow manganese oxide nanoparticles as positive T1 contrast agents for labeling and MRI tracking of adipose-derived mesenchymal stem cells. J. Am. Chem. Soc 2011, 133, 2955–2961. [Google Scholar]
  90. Chen, Y.; Yin, Q.; Ji, X.; Zhang, S.; Chen, H.; Zheng, Y.; Sun, Y.; Qu, H.; Wang, Z.; Li, Y.; et al. Manganese oxide-based multifunctionalized esoporous silica nanoparticles for pH-responsive MRI, ultrasonography and circumvention of MDR in cancer cells. Biomaterials 2012, 33, 7126–7137. [Google Scholar]
  91. Zhen, Z.; Xie, J. Development of manganese-based nanoparticles as contrast probes for magnetic resonance imaging. Theranostics 2012, 2, 45–54. [Google Scholar]
  92. Lee, Y.-C.; Chen, D.-Y.; Dodd, S.J.; Bouraoud, N.; Koretsky, A.P.; Krishnan, K.M. The use of silica coated MnO nanoparticles to control MRI relaxivity in response to specific physiological changes. Biomaterials 2012, 33, 3560–3567. [Google Scholar]
  93. Pan, D.; Schmieder, A.H.; Wickline, S.A.; Lanza, G.M. Manganese-based MRI contrast agents: Past, present, and future. Tetrahedron 2011, 67, 8431–8444. [Google Scholar]
  94. Chen, Y.; Chen, H.; Zhang, S.; Chen, F.; Sun, S.; He, Q.; Ma, M.; Wang, X.; Wu, H.; Zhang, L.; et al. Structure-property relationships in manganese oxide–mesoporous silica nanoparticles used for T1-weighted MRI and simultaneous anti-cancer drug delivery. Biomaterials 2012, 33, 2388–2398. [Google Scholar]
  95. Kuo, P.H.; Kanal, E.; Abu-Alfa, A.K.; Cowper, S.E. Gadolinium-based MR contrast agents and nephrogenic systemic fibrosis. Radiology 2007, 242, 647–649. [Google Scholar]
  96. Bulte, J.W.M.; Kraitchman, D.L. Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed 2004, 17, 484–499. [Google Scholar]
  97. Zhang, F.; Huang, X.; Qian, C.; Zhu, L.; Hida, N.; Niu, G.; Chen, X. Synergistic enhancement of iron oxide nanoparticle and gadolinium for dual-contrast MRI. Biochem. Biophys. Res. Commun 2012, 425, 886–891. [Google Scholar]
  98. Hu, F.; Jia, Q.; Li, Y.; Gao, M. Facile synthesis of ultrasmall PEGylated iron oxide nanoparticles for dual-contrast T1- and T2-weighted magnetic resonance imaging. Nanotechnology 2011, 22, 245604. [Google Scholar]
  99. Li, Z.; Yi, P.W.; Sun, Q.; Lei, H.; Zhao, H.L.; Zhu, Z.H.; Smith, S.C.; Lan, M.B.; Lu, G.Q.M. Ultrasmall water-soluble and biocompatible magnetic iron oxide nanoparticles as positive and negative dual contrast agents. Adv. Funct. Mater 2012, 22, 2387–2393. [Google Scholar]
  100. Zhou, Z.; Wang, L.; Chi, X.; Bao, J.; Yang, L.; Zhao, W.; Chen, Z.; Wang, X.; Chen, X.; Gao, J. Engineered iron-oxide-based nanoparticles as enhanced T1contrast agents for efficient tumor imaging. ACS Nano 2013, 7. [Google Scholar] [CrossRef]
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Figure 1. Electron configuration and magnetic moment of metal ions (adapted from Na et al. 2009 [29], Copyright 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim and reproduced with permission).
Figure 1. Electron configuration and magnetic moment of metal ions (adapted from Na et al. 2009 [29], Copyright 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim and reproduced with permission).
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Figure 2. Schematic representation of Gd-conjugated polydisulfide dendrimer clusters and corresponding MR images of nu/nu nude mice before and after the tail vein injection of Gd-conjugated polydisulfide dendrimer clusters (adapted from Huang et al. 2012 [60], Copyright 2012 American Chemical Society and reproduced with permission).
Figure 2. Schematic representation of Gd-conjugated polydisulfide dendrimer clusters and corresponding MR images of nu/nu nude mice before and after the tail vein injection of Gd-conjugated polydisulfide dendrimer clusters (adapted from Huang et al. 2012 [60], Copyright 2012 American Chemical Society and reproduced with permission).
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Figure 3. The results of the enhanced signal in different tissue in vivo [Magnevist® (a), PLA-PEG-PLL NPs (b) and Anti-VEGF PLA-PEG-PLL-Gd NPs (c)] (adapted from Liu et al. 2011 [55], Copyright 2011 Elsevier Ltd. and reproduced with permission).
Figure 3. The results of the enhanced signal in different tissue in vivo [Magnevist® (a), PLA-PEG-PLL NPs (b) and Anti-VEGF PLA-PEG-PLL-Gd NPs (c)] (adapted from Liu et al. 2011 [55], Copyright 2011 Elsevier Ltd. and reproduced with permission).
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Figure 4. Schematic representation of the synthetic route of NaLuF4@SiO2-GdDTPA nanoparticles (UCNP@SiO2-GdDTPA) (adapted from Xia et al. 2012 [64], Copyright 2012 Elsevier Ltd. and reproduced with permission).
Figure 4. Schematic representation of the synthetic route of NaLuF4@SiO2-GdDTPA nanoparticles (UCNP@SiO2-GdDTPA) (adapted from Xia et al. 2012 [64], Copyright 2012 Elsevier Ltd. and reproduced with permission).
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Figure 5. Schematic illustration of the designed nanostructure (a) and the synthesis procedure (b) of the Gd–Au DENPs. TEA and Ac2O represent triethylamine and acetic anhydride, respectively (adapted from Wen et al. 2013 [65], Copyright 2012 Elsevier Ltd. and reproduced with permission).
Figure 5. Schematic illustration of the designed nanostructure (a) and the synthesis procedure (b) of the Gd–Au DENPs. TEA and Ac2O represent triethylamine and acetic anhydride, respectively (adapted from Wen et al. 2013 [65], Copyright 2012 Elsevier Ltd. and reproduced with permission).
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Figure 6. Schematic illustration of the synthesis of HMnO@mSiO2 nanoparticles and labeling of mesenchymal stem cells (MSCs) and in vivo magnetic resonance imaging (MRI) of MSCs transplanted mouse (adapted from Kim et al. 2011 [89], Copyright 2011 American Chemical Society and reproduced with permission).
Figure 6. Schematic illustration of the synthesis of HMnO@mSiO2 nanoparticles and labeling of mesenchymal stem cells (MSCs) and in vivo magnetic resonance imaging (MRI) of MSCs transplanted mouse (adapted from Kim et al. 2011 [89], Copyright 2011 American Chemical Society and reproduced with permission).
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Figure 7. Schematic representation for the preparation of manganese oxide/mesoporous silica nanoparticles (MSNs)-based T1-weighted MRI contrast agents (adapted from Chen et al. 2012 [90], Copyright 2012 Elsevier Ltd. and reproduced with permission).
Figure 7. Schematic representation for the preparation of manganese oxide/mesoporous silica nanoparticles (MSNs)-based T1-weighted MRI contrast agents (adapted from Chen et al. 2012 [90], Copyright 2012 Elsevier Ltd. and reproduced with permission).
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MDPI and ACS Style

Zhu, D.; Liu, F.; Ma, L.; Liu, D.; Wang, Z. Nanoparticle-Based Systems for T1-Weighted Magnetic Resonance Imaging Contrast Agents. Int. J. Mol. Sci. 2013, 14, 10591-10607. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140510591

AMA Style

Zhu D, Liu F, Ma L, Liu D, Wang Z. Nanoparticle-Based Systems for T1-Weighted Magnetic Resonance Imaging Contrast Agents. International Journal of Molecular Sciences. 2013; 14(5):10591-10607. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140510591

Chicago/Turabian Style

Zhu, Derong, Fuyao Liu, Lina Ma, Dianjun Liu, and Zhenxin Wang. 2013. "Nanoparticle-Based Systems for T1-Weighted Magnetic Resonance Imaging Contrast Agents" International Journal of Molecular Sciences 14, no. 5: 10591-10607. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140510591

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