Next Article in Journal
Transition Pathway and Its Free-Energy Profile: A Protocol for Protein Folding Simulations
Previous Article in Journal
Non-Coding RNAs: Multi-Tasking Molecules in the Cell
Article

Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells

by 1, 2, 2 and 1,2,3,*
1
School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
2
Yue-yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
3
East Hospital, Tongji University, Shanghai 200120, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(8), 16040-16057; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140816040
Received: 17 May 2013 / Revised: 12 July 2013 / Accepted: 18 July 2013 / Published: 31 July 2013
(This article belongs to the Section Biochemistry)
Here, we report a natural chemical Matrine, which exhibits anti-melanoma potential with its PTEN activation mechanism. Matrine effectively inhibited proliferation of several carcinoma cell lines, including melanoma V600EBRAF harboring M21 cells. Flow cytometry analysis showed Matrine induced G0/G1 cell cycle arrest in M21 cells dose-dependently. Apoptosis in M21 cells induced by Matrine was identified by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis and Annexin-V/FITC staining. Molecular mechanistic study suggested that Matrine upregulated both mRNA level and protein expression level of phosphatase and tensin homolog deleted on chromosome ten (PTEN), leading to inhibition of the PI3K/Akt pathway. Downregulation of phosphor-Aktser473 by Matrine activated p21 and Bax, which contributed to G0/G1 cell cycle and apoptosis. Besides, Matrine enhanced the PI3K/Akt inhibition effects to inhibit the cell proliferation with PI3K inhibitor, LY2940002. In summary, our findings suggest Matrine is a promising antitumor drug candidate with its possible PTEN activation mechanisms for treating cancer diseases, such as melanomas. View Full-Text
Keywords: Matrine; cell cycle arrest; apoptosis; V600EBRAF melanoma; PTEN Matrine; cell cycle arrest; apoptosis; V600EBRAF melanoma; PTEN
Show Figures

MDPI and ACS Style

Jin, H.; Sun, Y.; Wang, S.; Cheng, X. Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells. Int. J. Mol. Sci. 2013, 14, 16040-16057. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140816040

AMA Style

Jin H, Sun Y, Wang S, Cheng X. Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells. International Journal of Molecular Sciences. 2013; 14(8):16040-16057. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140816040

Chicago/Turabian Style

Jin, Hui, Yu Sun, Shuiying Wang, and Xiaodong Cheng. 2013. "Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells" International Journal of Molecular Sciences 14, no. 8: 16040-16057. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms140816040

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop