Next Article in Journal
Soy and Breast Cancer: Focus on Angiogenesis
Next Article in Special Issue
Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells
Previous Article in Journal
MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1
Previous Article in Special Issue
Investigation of Förster Resonance Energy Transfer (FRET) and Competition of Fluorescent Dyes on DNA Microparticles
Article

Intravital FRET: Probing Cellular and Tissue Function in Vivo

1
Neuropathology, Charité–University of Medicine, Berlin 10117, Germany
2
Germany German Rheumatism Research Center, Berlin 10117, Germany
3
Immundynamics and Intravital Microscopy, Charité–University of Medicine, Berlin 10117, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Herbert Schneckenburger
Int. J. Mol. Sci. 2015, 16(5), 11713-11727; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160511713
Received: 20 February 2015 / Accepted: 13 May 2015 / Published: 21 May 2015
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET) 2015)
The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and show their concrete application in the context of neuroinflammation in adult mice. View Full-Text
Keywords: intravital FRET; multi-photon microscopy; fluorescence lifetime imaging; genetically encoded calcium indicators intravital FRET; multi-photon microscopy; fluorescence lifetime imaging; genetically encoded calcium indicators
Show Figures

Graphical abstract

MDPI and ACS Style

Radbruch, H.; Bremer, D.; Mothes, R.; Günther, R.; Rinnenthal, J.L.; Pohlan, J.; Ulbricht, C.; Hauser, A.E.; Niesner, R. Intravital FRET: Probing Cellular and Tissue Function in Vivo. Int. J. Mol. Sci. 2015, 16, 11713-11727. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160511713

AMA Style

Radbruch H, Bremer D, Mothes R, Günther R, Rinnenthal JL, Pohlan J, Ulbricht C, Hauser AE, Niesner R. Intravital FRET: Probing Cellular and Tissue Function in Vivo. International Journal of Molecular Sciences. 2015; 16(5):11713-11727. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160511713

Chicago/Turabian Style

Radbruch, Helena, Daniel Bremer, Ronja Mothes, Robert Günther, Jan L. Rinnenthal, Julian Pohlan, Carolin Ulbricht, Anja E. Hauser, and Raluca Niesner. 2015. "Intravital FRET: Probing Cellular and Tissue Function in Vivo" International Journal of Molecular Sciences 16, no. 5: 11713-11727. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160511713

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop