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MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF

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Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia
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Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia
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Department of Applied Biology, College of Sciences, University of Sharjah, Emirates of Sharjah, P.O. Box 27272, United Arab Emirates
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Sharjah Institute for Medical Research, University of Sharjah, Emirates of Sharjah, P.O. Box 27272, United Arab Emirates
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Director, Center for Neuroscience Services and Research, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia
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Jabatan Neurosains, Hospital Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia
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Department of Medical Microbiology, School of Medical Sciences, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Wilhelm Bloch
Int. J. Mol. Sci. 2015, 16(5), 9693-9718; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms16059693
Received: 14 January 2015 / Revised: 17 April 2015 / Accepted: 17 April 2015 / Published: 29 April 2015
(This article belongs to the Special Issue Stem Cell Activation in Adult Organism)
Insulin-like growth factor 1 (IGF-1) enhances cellular proliferation and reduces apoptosis during the early differentiation of bone marrow derived mesenchymal stem cells (BMSCs) into neural progenitor-like cells (NPCs) in the presence of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). BMSCs were differentiated in three groups of growth factors: (A) EGF + bFGF, (B) EGF + bFGF + IGF-1, and (C) without growth factor. To unravel the molecular mechanisms of the NPCs derivation, microarray analysis using GeneChip® miRNA arrays was performed. The profiles were compared among the groups. Annotated microRNA fingerprints (GSE60060) delineated 46 microRNAs temporally up-regulated or down-regulated compared to group C. The expressions of selected microRNAs were validated by real-time PCR. Among the 46 microRNAs, 30 were consistently expressed for minimum of two consecutive time intervals. In Group B, only miR-496 was up-regulated and 12 microRNAs, including the let-7 family, miR-1224, miR-125a-3p, miR-214, miR-22, miR-320, miR-708, and miR-93, were down-regulated. Bioinformatics analysis reveals that some of these microRNAs (miR-22, miR-214, miR-125a-3p, miR-320 and let-7 family) are associated with reduction of apoptosis. Here, we summarize the roles of key microRNAs associated with IGF-1 in the differentiation of BMSCs into NPCs. These findings may provide clues to further our understanding of the mechanisms and roles of microRNAs as key regulators of BMSC-derived NPC maintenance. View Full-Text
Keywords: microRNA; IGF-1; microarray; bone marrow; mesenchymal stem cell; neural progenitor cell; proliferation; apoptosis microRNA; IGF-1; microarray; bone marrow; mesenchymal stem cell; neural progenitor cell; proliferation; apoptosis
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MDPI and ACS Style

Huat, T.J.; Khan, A.A.; Abdullah, J.M.; Idris, F.M.; Jaafar, H. MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF. Int. J. Mol. Sci. 2015, 16, 9693-9718. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms16059693

AMA Style

Huat TJ, Khan AA, Abdullah JM, Idris FM, Jaafar H. MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF. International Journal of Molecular Sciences. 2015; 16(5):9693-9718. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms16059693

Chicago/Turabian Style

Huat, Tee J., Amir A. Khan, Jafri M. Abdullah, Fauziah M. Idris, and Hasnan Jaafar. 2015. "MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF" International Journal of Molecular Sciences 16, no. 5: 9693-9718. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms16059693

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