Next Article in Journal
Disorder Prediction Methods, Their Applicability to Different Protein Targets and Their Usefulness for Guiding Experimental Studies
Next Article in Special Issue
BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype
Previous Article in Journal
Angiogenesis-Related Biomarkers (sFlt-1/PLGF) in the Prediction and Diagnosis of Placental Dysfunction: An Approach for Clinical Integration
Previous Article in Special Issue
Therapeutic Potential of Differentiated Mesenchymal Stem Cells for Treatment of Osteoarthritis
Article

Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts

Amorepacific Corporation R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Geyonggi-do 446729, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Wilhelm Bloch
Int. J. Mol. Sci. 2015, 16(8), 19027-19039; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819027
Received: 6 July 2015 / Revised: 27 July 2015 / Accepted: 27 July 2015 / Published: 13 August 2015
(This article belongs to the Special Issue Stem Cell Activation in Adult Organism)
Adult skin stem cells are recognized as potential therapeutics to rejuvenate aged skin. We previously demonstrated that human dermal stem/progenitor cells (hDSPCs) with multipotent capacity could be enriched from human dermal fibroblasts using collagen type IV. However, the effects of hDSPCs on cellular senescence remain to be elucidated. In the present study, we investigated whether conditioned medium (CM) collected from hDSPC cultures (hDSPC-CM) exhibits beneficial effects on senescent fibroblasts. We found that hDSPC-CM promoted proliferation and decreased the expression level of senescence-associated β-galactosidase in senescent fibroblasts. In addition, p53 phosphorylation and p21 expression were significantly reduced in senescent fibroblasts treated with hDSPC-CM. hDSPC-CM restored the expression levels of collagen type I, collagen type III, and tissue inhibitor of metalloproteinase, and antagonized the increase of matrix metalloproteinase 1 expression. Finally, we demonstrated that hDSPC-CM significantly reduced reactive oxygen species levels by specifically up-regulating the expression level of superoxide dismutase 2. Taken together, these data suggest that hDSPC-CM can be applied as a potential therapeutic agent for improving human aged skin. View Full-Text
Keywords: fibroblast; human dermal stem/progenitor cells; senescence fibroblast; human dermal stem/progenitor cells; senescence
Show Figures

Graphical abstract

MDPI and ACS Style

Jung, J.-Y.; Shim, J.H.; Choi, H.; Lee, T.R.; Shin, D.W. Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts. Int. J. Mol. Sci. 2015, 16, 19027-19039. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819027

AMA Style

Jung J-Y, Shim JH, Choi H, Lee TR, Shin DW. Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts. International Journal of Molecular Sciences. 2015; 16(8):19027-19039. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819027

Chicago/Turabian Style

Jung, Ji-Yong, Joong H. Shim, Hyun Choi, Tae R. Lee, and Dong W. Shin 2015. "Human Dermal Stem/Progenitor Cell-Derived Conditioned Medium Improves Senescent Human Dermal Fibroblasts" International Journal of Molecular Sciences 16, no. 8: 19027-19039. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819027

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop