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Article

Molecular and Functional Characterization of Thioredoxin 1from Korean Rose Bitterling (Rhodeus uyekii)

Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea
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Academic Editor: Li Lin
Int. J. Mol. Sci. 2015, 16(8), 19433-19446; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819433
Received: 13 July 2015 / Revised: 6 August 2015 / Accepted: 11 August 2015 / Published: 17 August 2015
(This article belongs to the Special Issue Fish Molecular Biology)
Thioredoxin is a multifunctional antioxidant enzyme that belongs to the reductase family. In this study, we cloned and characterized thioredoxin 1 cDNA from the Korean rose bitterling Rhodeus uyekii (RuTrx). The full-length RuTrx cDNA consists of 674 bp with a 324 nt open reading frame (ORF) encoding a 107 aa protein. The deduced RuTrx amino acid sequence indicated a characteristic redox active site, 31WCGPC35. Pairwise alignment revealed RuTrx amino acid identity (55.1%–83.2%) with orthologs from various species of mammalia, amphibia, fish and bird. Phylogenetic analysis was conducted to determine the evolutionary position of RuTrx. Expression analysis showed that RuTrx transcripts were present in all of the tissues examined, and was high in the hepatopancreas of R. uyekii. During early development, the expression of RuTrx transcripts was increased. Recombinant RuTrx protein (rRuTrx) was tested for its capacity to serve as an antioxidant enzyme using a metal-catalyzed oxidation (MCO) system. The ability of rRuTrx to protect against supercoiled DNA cleavage due to oxidative nicking increased in a dose-dependent manner. In Raw264.7 cells, Dihydroethidium (DHE) staining for ROS production indicated the antioxidant activity of rRuTrx. Together, these findings suggest that RuTrx may play a role in maintaining the redox state balance in Korean rose bitterling R. uyekii. View Full-Text
Keywords: thioredoxin; Korean rose bitterling; Rhodues uyekii; expression analysis; anti-oxidant; MCO assay; ROS detection thioredoxin; Korean rose bitterling; Rhodues uyekii; expression analysis; anti-oxidant; MCO assay; ROS detection
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MDPI and ACS Style

Kim, J.; Moon, J.Y.; Kim, W.-J.; Kim, D.-G.; Nam, B.-H.; Kim, Y.-O.; Park, J.Y.; An, C.M.; Kong, H.J. Molecular and Functional Characterization of Thioredoxin 1from Korean Rose Bitterling (Rhodeus uyekii). Int. J. Mol. Sci. 2015, 16, 19433-19446. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819433

AMA Style

Kim J, Moon JY, Kim W-J, Kim D-G, Nam B-H, Kim Y-O, Park JY, An CM, Kong HJ. Molecular and Functional Characterization of Thioredoxin 1from Korean Rose Bitterling (Rhodeus uyekii). International Journal of Molecular Sciences. 2015; 16(8):19433-19446. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819433

Chicago/Turabian Style

Kim, Julan, Ji Y. Moon, Woo-Jin Kim, Dong-Gyun Kim, Bo-Hye Nam, Young-Ok Kim, Jung Y. Park, Cheul M. An, and Hee J. Kong 2015. "Molecular and Functional Characterization of Thioredoxin 1from Korean Rose Bitterling (Rhodeus uyekii)" International Journal of Molecular Sciences 16, no. 8: 19433-19446. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160819433

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