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Article

Modulation of the PI3K/Akt Pathway and Bcl-2 Family Proteins Involved in Chicken’s Tubular Apoptosis Induced by Nickel Chloride (NiCl2)

1
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China
2
College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China
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Author to whom correspondence should be addressed.
Academic Editor: Reinhard Dallinger
Int. J. Mol. Sci. 2015, 16(9), 22989-23011; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160922989
Received: 17 August 2015 / Revised: 14 September 2015 / Accepted: 17 September 2015 / Published: 23 September 2015
(This article belongs to the Special Issue Metal Metabolism in Animals)
Exposure of people and animals to environments highly polluted with nickel (Ni) can cause pathologic effects. Ni compounds can induce apoptosis, but the mechanism and the pathway of Ni compounds-induced apoptosis are unclear. We evaluated the alterations of apoptosis, mitochondrial membrane potential (MMP), phosphoinositide-3-kinase (PI3K)/serine-threonine kinase (Akt) pathway, and Bcl-2 family proteins induced by nickel chloride (NiCl2) in the kidneys of broiler chickens, using flow cytometry, terminal deoxynucleotidyl transferase 2ʹ-deoxyuridine 5ʹ-triphosphate dUTP nick end-labeling (TUNEL), immunohistochemstry and quantitative real-time polymerase chain reaction (qRT-PCR). We found that dietary NiCl2 in excess of 300 mg/kg resulted in a significant increase in apoptosis, which was associated with decrease in MMP, and increase in apoptosis inducing factor (AIF) and endonuclease G (EndoG) protein and mRNA expression. Concurrently, NiCl2 inhibited the PI3K/Akt pathway, which was characterized by decreasing PI3K, Akt1 and Akt2 mRNA expression levels. NiCl2 also reduced the protein and mRNA expression of anti-apoptotic Bcl-2 and Bcl-xL and increased the protein and mRNA expression of pro-apoptotic Bax and Bak. These results show that NiCl2 causes mitochondrial-mediated apoptosis by disruption of MMP and increased expression of AIF and EndoG mRNA and protein, and that the underlying mechanism of MMP loss involves the Bcl-2 family proteins modulation and PI3K/Akt pathway inhibition. View Full-Text
Keywords: NiCl2; apoptosis; PI3K/Akt pathway; Bcl-2; mitochondria NiCl2; apoptosis; PI3K/Akt pathway; Bcl-2; mitochondria
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MDPI and ACS Style

Guo, H.; Cui, H.; Peng, X.; Fang, J.; Zuo, Z.; Deng, J.; Wang, X.; Wu, B.; Chen, K.; Deng, J. Modulation of the PI3K/Akt Pathway and Bcl-2 Family Proteins Involved in Chicken’s Tubular Apoptosis Induced by Nickel Chloride (NiCl2). Int. J. Mol. Sci. 2015, 16, 22989-23011. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160922989

AMA Style

Guo H, Cui H, Peng X, Fang J, Zuo Z, Deng J, Wang X, Wu B, Chen K, Deng J. Modulation of the PI3K/Akt Pathway and Bcl-2 Family Proteins Involved in Chicken’s Tubular Apoptosis Induced by Nickel Chloride (NiCl2). International Journal of Molecular Sciences. 2015; 16(9):22989-23011. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160922989

Chicago/Turabian Style

Guo, Hongrui, Hengmin Cui, Xi Peng, Jing Fang, Zhicai Zuo, Junliang Deng, Xun Wang, Bangyuan Wu, Kejie Chen, and Jie Deng. 2015. "Modulation of the PI3K/Akt Pathway and Bcl-2 Family Proteins Involved in Chicken’s Tubular Apoptosis Induced by Nickel Chloride (NiCl2)" International Journal of Molecular Sciences 16, no. 9: 22989-23011. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms160922989

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