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Article

Age-Related Renal Microvascular Changes: Evaluation by Three-Dimensional Digital Imaging of the Human Renal Microcirculation Using Virtual Microscopy

1
Department of Kidney and Vascular Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8571, Japan
2
Laboratory of Food and Physiological Sciences, Azabu University, Sagamihara 252-5201, Japan
3
Division of Pathology, Tsukuba Medical Center, Tsukuba 305-0005, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Michael Henein and William Chi-shing Cho
Int. J. Mol. Sci. 2016, 17(11), 1831; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17111831
Received: 31 August 2016 / Revised: 21 October 2016 / Accepted: 27 October 2016 / Published: 2 November 2016
(This article belongs to the Special Issue Atherosclerosis and Vascular Imaging 2016)
The renal microvasculature is targeted during aging, sometimes producing chronic kidney disease (CKD). Overdiagnosis of CKD in older persons is concerning. To prevent it, a new concept of “healthy aging” is arising from a healthy renal donor study. We investigated the renal microcirculatory changes of three older persons and compared them with that of one patient with nephrosclerosis using a three-dimensional (3D) reconstruction technique that we previously developed. This method uses a virtual slide system and paraffin-embedded serial sections of surgical material that was double-immunostained by anti-CD34 and anti-α smooth muscle actin (SMA) antibodies for detecting endothelial cells and medial smooth muscle cells, respectively. In all cases, the 3D images proved that arteriosclerotic changes in large proximal interlobular arteries did not directly induce distal arterial change or glomerulosclerosis. The nephrosclerotic patient showed severe hyalinosis with luminal narrowing of small arteries directly inducing glomerulosclerosis. We also visualized an atubular glomerulus and intraglomerular dilatation of an afferent arteriole during healthy aging on the 3D image and showed that microcirculatory changes were responsible for them. Thus, we successfully visualized healthy aged kidneys on 3D images and confirmed the underlying pathology. This method has the ability to investigate renal microcirculatory damage during healthy aging. View Full-Text
Keywords: human renal microcirculation; healthy aging; paraffin-embedded sections; three-dimensional imaging; virtual microscopy human renal microcirculation; healthy aging; paraffin-embedded sections; three-dimensional imaging; virtual microscopy
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MDPI and ACS Style

Uesugi, N.; Shimazu, Y.; Kikuchi, K.; Nagata, M. Age-Related Renal Microvascular Changes: Evaluation by Three-Dimensional Digital Imaging of the Human Renal Microcirculation Using Virtual Microscopy. Int. J. Mol. Sci. 2016, 17, 1831. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17111831

AMA Style

Uesugi N, Shimazu Y, Kikuchi K, Nagata M. Age-Related Renal Microvascular Changes: Evaluation by Three-Dimensional Digital Imaging of the Human Renal Microcirculation Using Virtual Microscopy. International Journal of Molecular Sciences. 2016; 17(11):1831. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17111831

Chicago/Turabian Style

Uesugi, Noriko, Yoshihito Shimazu, Kazunori Kikuchi, and Michio Nagata. 2016. "Age-Related Renal Microvascular Changes: Evaluation by Three-Dimensional Digital Imaging of the Human Renal Microcirculation Using Virtual Microscopy" International Journal of Molecular Sciences 17, no. 11: 1831. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17111831

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