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Chalcone-Induced Apoptosis through Caspase-Dependent Intrinsic Pathways in Human Hepatocellular Carcinoma Cells

Laboratorio de Bionanotecnologia, Universidad Bernardo O Higgins, General Gana 1780, Santiago 8370854, Chile
Departamento de Ciencias Químicas, Laboratorio de Síntesis Orgánica, Universidad Andres Bello, Av. República 275, Santiago 8370146, Chile
Centro de Investigación y Tratamiento del Cancer, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile
Center for Excellence in Precision Medicine Pfizer, Pfizer Chile, Obispo Arturo Espinoza Campos 2526, Macul, Santiago 7810305, Chile
Urology Department, Hospital Barros Luco Trudeau, San Miguel, Santiago 8900085, Chile
Andes Biotechnologies SA and Fundación Ciencia para la Vida, Zañartu 1482, Ñuñoa, Santiago 7780272, Chile
Department of Biological Science, Faculty of Biological Science, Universidad Andrés Bello, Santiago 8370146, Chile
Instituto Nacional del Cancer, Universidad de Chile, Profesor Zañartu 1010, Santiago 8380455, Chile
Laboratorio de Fisiopatología Integrativa, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas and Facultad de Medicina, Universidad Andres Bello, Avenida Republica 239, Santiago 8370146, Chile
Millennium Institute on Immunology and Immunotherapy, Santiago 8331150, Chile
Author to whom correspondence should be addressed.
Academic Editor: Johannes Haybaeck
Int. J. Mol. Sci. 2016, 17(2), 260;
Received: 24 December 2015 / Revised: 22 January 2016 / Accepted: 1 February 2016 / Published: 22 February 2016
(This article belongs to the Collection Molecular Mechanisms of Human Liver Diseases)
Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers worldwide. Chemoprevention of HCC can be achieved through the use of natural or synthetic compounds that reverse, suppress or prevent the development of cancer progression. In this study, we investigated the antiproliferative effects and the mechanism of action of two compounds, 2,3,4′-trimethoxy-2′-hydroxy-chalcone (CH1) and 3′-bromo-3,4-dimethoxy-chalcone (CH2), over human hepatoma cells (HepG2 and Huh-7) and cultured mouse hepatocytes (HepM). Cytotoxic effects were observed over the HepG2 and Huh-7, and no effects were observed over the HepM. For HepG2 cells, treated separately with each chalcone, typical apoptotic laddering and nuclear condensation were observed. Additionally, the caspases and Bcl-2 family proteins activation by using Western blotting and immunocytochemistry were studied. Caspase-8 was not activated, but caspase-3 and -9 were both activated by chalcones in HepG2 cells. Chalcones also induced reactive oxygen species (ROS) accumulation after 4, 8 and 24 h of treatment in HepG2 cells. These results suggest that apoptosis in HepG2 was induced through: (i) a caspase-dependent intrinsic pathway; and (ii) by alterations in the cellular levels of Bcl-2 family proteins, and also, that the chalcone moiety could be a potent candidate as novel anticancer agents acting on human hepatomas. View Full-Text
Keywords: Chalcone; Caspase; Reactive Oxygen Species Chalcone; Caspase; Reactive Oxygen Species
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MDPI and ACS Style

Ramirez-Tagle, R.; Escobar, C.A.; Romero, V.; Montorfano, I.; Armisén, R.; Borgna, V.; Jeldes, E.; Pizarro, L.; Simon, F.; Echeverria, C. Chalcone-Induced Apoptosis through Caspase-Dependent Intrinsic Pathways in Human Hepatocellular Carcinoma Cells. Int. J. Mol. Sci. 2016, 17, 260.

AMA Style

Ramirez-Tagle R, Escobar CA, Romero V, Montorfano I, Armisén R, Borgna V, Jeldes E, Pizarro L, Simon F, Echeverria C. Chalcone-Induced Apoptosis through Caspase-Dependent Intrinsic Pathways in Human Hepatocellular Carcinoma Cells. International Journal of Molecular Sciences. 2016; 17(2):260.

Chicago/Turabian Style

Ramirez-Tagle, Rodrigo, Carlos A. Escobar, Valentina Romero, Ignacio Montorfano, Ricardo Armisén, Vincenzo Borgna, Emanuel Jeldes, Luis Pizarro, Felipe Simon, and Cesar Echeverria. 2016. "Chalcone-Induced Apoptosis through Caspase-Dependent Intrinsic Pathways in Human Hepatocellular Carcinoma Cells" International Journal of Molecular Sciences 17, no. 2: 260.

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