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Article

Mesenchymal Stem Cells Increase Neo-Angiogenesis and Albumin Production in a Liver Tissue-Engineered Engraftment

1
Department of General Surgery and Odontoiatrics, Liver Transplant Unit, University Hospital of Verona, P.le Aristide Stefani 1, Verona 37126, Italy
2
Nanomedicine Lab, Institute of Inflammation and Repair, Faculty of Medical & Human Sciences, University of Manchester, Manchester M13 9PT, UK
3
Department of Biomedical Sciences, University of Padova, Viale Giuseppe Colombo, 3, Padova 35131, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2016, 17(3), 374; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030374
Received: 25 November 2015 / Revised: 12 February 2016 / Accepted: 1 March 2016 / Published: 12 March 2016
(This article belongs to the Special Issue Advances in Cell Transplantation)
The construction of a three-dimensional (3D) liver tissue is limited by many factors; one of them is the lack of vascularization inside the tissue-engineered construct. An engineered liver pocket-scaffold able to increase neo-angiogenesis in vivo could be a solution to overcome these limitations. In this work, a hyaluronan (HA)-based scaffold enriched with human mesenchymal stem cells (hMSCs) and rat hepatocytes was pre-conditioned in a bioreactor system, then implanted into the liver of rats. Angiogenesis and hepatocyte metabolic functions were monitored. The formation of a de novo vascular network within the HA-based scaffold, as well as an improvement in albumin production by the implanted hepatocytes, were detected. The presence of hMSCs in the HA-scaffold increased the concentration of growth factors promoting angiogenesis inside the graft. This event ensured a high blood vessel density, coupled with a support to metabolic functions of hepatocytes. All together, these results highlight the important role played by stem cells in liver tissue-engineered engraftment. View Full-Text
Keywords: mesenchymal stem cell; hepatocyte; angiogenesis; hyaluronan-based scaffold; engineered liver tissue mesenchymal stem cell; hepatocyte; angiogenesis; hyaluronan-based scaffold; engineered liver tissue
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MDPI and ACS Style

Carraro, A.; Buggio, M.; Gardin, C.; Tedeschi, U.; Ferroni, L.; Zavan, B. Mesenchymal Stem Cells Increase Neo-Angiogenesis and Albumin Production in a Liver Tissue-Engineered Engraftment. Int. J. Mol. Sci. 2016, 17, 374. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030374

AMA Style

Carraro A, Buggio M, Gardin C, Tedeschi U, Ferroni L, Zavan B. Mesenchymal Stem Cells Increase Neo-Angiogenesis and Albumin Production in a Liver Tissue-Engineered Engraftment. International Journal of Molecular Sciences. 2016; 17(3):374. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030374

Chicago/Turabian Style

Carraro, Amedeo, Maurizio Buggio, Chiara Gardin, Umberto Tedeschi, Letizia Ferroni, and Barbara Zavan. 2016. "Mesenchymal Stem Cells Increase Neo-Angiogenesis and Albumin Production in a Liver Tissue-Engineered Engraftment" International Journal of Molecular Sciences 17, no. 3: 374. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17030374

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