Next Article in Journal
Cell Type-Specific Modulation of Respiratory Chain Supercomplex Organization
Previous Article in Journal
Enhancement of Anti-Inflammatory Activity of Curcumin Using Phosphatidylserine-Containing Nanoparticles in Cultured Macrophages
Previous Article in Special Issue
Toxicological Considerations, Toxicity Assessment, and Risk Management of Inhaled Nanoparticles
Article

Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles

1
Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, China
2
Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
*
Author to whom correspondence should be addressed.
Academic Editors: Michael Routledge and Bing Yan
Int. J. Mol. Sci. 2016, 17(6), 974; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17060974
Received: 29 February 2016 / Revised: 13 May 2016 / Accepted: 14 June 2016 / Published: 21 June 2016
(This article belongs to the Special Issue Cellular Toxicity of Nanoparticles)
Extensive utilization increases the exposure of humans to Ag nanoparticles (NPs) via the oral pathway. To comprehensively address the action of Ag NPs to the gastrointestinal systems in real situations, i.e., the long-term low-dose exposure, we evaluated and compared the toxicity of three Ag NPs (20–30 nm with different surface coatings) to the human intestine cell Caco-2 after 1-day and 21-day exposures, using various biological assays. In both the short- and long-term exposures, the variety of surface coating predominated the toxicity of Ag NPs in a descending order of citrate-coated Ag NP (Ag-CIT), bare Ag NP (Ag-B), and poly (N-vinyl-2-pyrrolidone)-coated Ag NP (Ag-PVP). The short-term exposure induced cell growth inhibition and death. The cell viability loss appeared after cells were exposed to 0.7 μg/mL Ag-CIT, 0.9 μg/mL Ag-B or >1.0 μg/mL Ag-PVP for 24 h. The short-term and higher-dose exposure also induced reactive oxygen species (ROS) generation, mitochondrial damage, cell membrane leakage, apoptosis, and inflammation (IL-8 level). The long-term exposure only inhibited the cell proliferation. After 21-day exposure to 0.4 μg/mL Ag-CIT, the cell viability dropped to less than 50%, while cells exposed to 0.5 μg/mL Ag-PVP remained normal as the control. Generally, 0.3 μg/mL is the non-toxic dose for the long-term exposure of Caco-2 cells to Ag NPs in this study. However, cells presented inflammation after exposure to Ag NPs with the non-toxic dose in the long-term exposure. View Full-Text
Keywords: Ag nanoparticle; Caco-2 cells; surface coating; long-term; low-dose; toxicity Ag nanoparticle; Caco-2 cells; surface coating; long-term; low-dose; toxicity
Show Figures

Graphical abstract

MDPI and ACS Style

Chen, N.; Song, Z.-M.; Tang, H.; Xi, W.-S.; Cao, A.; Liu, Y.; Wang, H. Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles. Int. J. Mol. Sci. 2016, 17, 974. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17060974

AMA Style

Chen N, Song Z-M, Tang H, Xi W-S, Cao A, Liu Y, Wang H. Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles. International Journal of Molecular Sciences. 2016; 17(6):974. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17060974

Chicago/Turabian Style

Chen, Ni, Zheng-Mei Song, Huan Tang, Wen-Song Xi, Aoneng Cao, Yuanfang Liu, and Haifang Wang. 2016. "Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles" International Journal of Molecular Sciences 17, no. 6: 974. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17060974

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop