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Article

Bone Marrow Mesenchymal Stem Cells Expressing Baculovirus-Engineered Bone Morphogenetic Protein-7 Enhance Rabbit Posterolateral Fusion

Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 333, Taiwan
Academic Editor: Maurizio Muraca
Int. J. Mol. Sci. 2016, 17(7), 1073; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17071073
Received: 28 March 2016 / Revised: 20 June 2016 / Accepted: 27 June 2016 / Published: 5 July 2016
(This article belongs to the Special Issue Advances in Cell Transplantation)
Previous studies have suggested that bone marrow-derived mesenchymal stem cells (BMDMSCs) genetically modified with baculoviral bone morphogenetic protein-2 (Bac-BMP-2) vectors could achieve successful fusion in a femur defect model or in a spinal fusion model. In this study, BMDMSCs expressing BMP-7 (Bac-BMP-7-BMDMSCs) were generated. We hypothesized that Bac-BMP-7-BMDMSCs could secrete more BMP-7 than untransduced BMDMSCs in vitro and achieve spinal posterolateral fusion in a rabbit model. Eighteen rabbits underwent posterolateral fusion at L4-5. Group I (n = 6) was implanted with collagen-β-tricalcium phosphate (TCP)-hydroxyapatite (HA), Group II (n = 6) was implanted with collagen-β-TCP-HA plus BMDMSCs, and Group III (n = 6) was implanted with collagen-β-TCP-HA plus Bac-BMP-7-BMDMSCs. In vitro production of BMP-7 was quantified with an enzyme-linked immunosorbent assay (ELISA). Spinal fusion was examined using computed tomography (CT), manual palpation, and histological analysis. ELISA demonstrated that Bac-BMP-7-BMDMSCs produced four-fold to five-fold more BMP-7 than did BMDMSCs. In the CT results, 6 fused segments were observed in Group I (50%, 6/12), 8 in Group II (67%, 8/12), and 12 in Group III (100%, 12/12). The fusion rate, determined by manual palpation, was 0% (0/6) in Group I, 0% (0/6) in Group II, and 83% (5/6) in Group III. Histology showed that Group III had more new bone and matured marrow formation. In conclusion, BMDMSCs genetically transduced with the Bac-BMP-7 vector could express more BMP-7 than untransduced BMDMSCs. These Bac-BMP-7-BMDMSCs on collagen-β-TCP-HA scaffolds were able to induce successful spinal fusion in rabbits. View Full-Text
Keywords: bone marrow stem cell; bone morphogenetic protein 7; baculovirus; spinal fusion; rabbit bone marrow stem cell; bone morphogenetic protein 7; baculovirus; spinal fusion; rabbit
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MDPI and ACS Style

Liao, J.-C. Bone Marrow Mesenchymal Stem Cells Expressing Baculovirus-Engineered Bone Morphogenetic Protein-7 Enhance Rabbit Posterolateral Fusion. Int. J. Mol. Sci. 2016, 17, 1073. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17071073

AMA Style

Liao J-C. Bone Marrow Mesenchymal Stem Cells Expressing Baculovirus-Engineered Bone Morphogenetic Protein-7 Enhance Rabbit Posterolateral Fusion. International Journal of Molecular Sciences. 2016; 17(7):1073. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17071073

Chicago/Turabian Style

Liao, Jen-Chung. 2016. "Bone Marrow Mesenchymal Stem Cells Expressing Baculovirus-Engineered Bone Morphogenetic Protein-7 Enhance Rabbit Posterolateral Fusion" International Journal of Molecular Sciences 17, no. 7: 1073. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17071073

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