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Article

Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro

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International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town Component, Anzio Road, Observatory, Cape Town 7925, South Africa
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Division of Medical Biochemistry, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa
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Department of Immunology, Institute for Cellular and Molecular Medicine, South African Medical Research Council (SAMRC) Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria 0002, South Africa
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Division of Human Genetics, Department of Pathology, Institute of Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa
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Division of Immunology, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Mohamed N. Rahaman
Int. J. Mol. Sci. 2016, 17(8), 1259; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081259
Received: 5 July 2016 / Revised: 17 July 2016 / Accepted: 25 July 2016 / Published: 3 August 2016
(This article belongs to the Section Materials Science)
Mesenchymal stromal/stem cells (MSCs) represent an area being intensively researched for tissue engineering and regenerative medicine applications. MSCs may provide the opportunity to treat diseases and injuries that currently have limited therapeutic options, as well as enhance present strategies for tissue repair. The cellular environment has a significant role in cellular development and differentiation through cell–matrix interactions. The aim of this study was to investigate the behavior of adipose-derived MSCs (ad-MSCs) in the context of a cell-derived matrix so as to model the in vivo physiological microenvironment. The fibroblast-derived extracellular matrix (fd-ECM) did not affect ad-MSC morphology, but reduced ad-MSC proliferation. Ad-MSCs cultured on fd-ECM displayed decreased expression of integrins α2 and β1 and subsequently lost their multipotency over time, as shown by the decrease in CD44, Octamer-binding transcription factor 4 (OCT4), SOX2, and NANOG gene expression. The fd-ECM induced chondrogenic differentiation in ad-MSCs compared to control ad-MSCs. Loss of function studies, through the use of siRNA and a mutant Notch1 construct, revealed that ECM-mediated ad-MSCs chondrogenesis requires Notch1 and β-catenin signaling. The fd-ECM also showed anti-senescence effects on ad-MSCs. The fd-ECM is a promising approach for inducing chondrogenesis in ad-MSCs and chondrogenic differentiated ad-MSCs could be used in stem cell therapy procedures. View Full-Text
Keywords: mesenchymal stromal/stem cells; regenerative medicine; three-dimensional; extracellular matrix; differentiation; chondrogenesis mesenchymal stromal/stem cells; regenerative medicine; three-dimensional; extracellular matrix; differentiation; chondrogenesis
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MDPI and ACS Style

Dzobo, K.; Turnley, T.; Wishart, A.; Rowe, A.; Kallmeyer, K.; Van Vollenstee, F.A.; Thomford, N.E.; Dandara, C.; Chopera, D.; Pepper, M.S.; Parker, M.I. Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro. Int. J. Mol. Sci. 2016, 17, 1259. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081259

AMA Style

Dzobo K, Turnley T, Wishart A, Rowe A, Kallmeyer K, Van Vollenstee FA, Thomford NE, Dandara C, Chopera D, Pepper MS, Parker MI. Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro. International Journal of Molecular Sciences. 2016; 17(8):1259. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081259

Chicago/Turabian Style

Dzobo, Kevin, Taegyn Turnley, Andrew Wishart, Arielle Rowe, Karlien Kallmeyer, Fiona A. Van Vollenstee, Nicholas E. Thomford, Collet Dandara, Denis Chopera, Michael S. Pepper, and M. I. Parker 2016. "Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro" International Journal of Molecular Sciences 17, no. 8: 1259. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081259

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