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Article

Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus

1
Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
2
Fisheries Technology Extension Station of Beijing, Beijing 101105, China
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Int. J. Mol. Sci. 2016, 17(8), 1299; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081299
Received: 6 June 2016 / Revised: 21 July 2016 / Accepted: 26 July 2016 / Published: 22 August 2016
(This article belongs to the Section Biochemistry)
In this study, we selected natural polyploidy loach (diploid, triploid and tetraploid) and hybrid F1 generation obverse cross (4 × 2) and inverse cross (2 × 4) by diploids and tetraploids as the research model. The MSAP (methylation-sensitive amplified polymorphism) reaction system was established by our laboratory to explore methylation levels and pattern diversification features at the whole genome level of the polyploidy loach. The results showed that the total methylation and full methylation rates decreased on increased ploidy individuals; moreover, the hemimethylation rate showed no consistent pattern. Compared with diploid loach, the methylation patterns of tetraploid sites changed 68.17%, and the methylation patterns of triploid sites changed 73.05%. The proportion of hypermethylation genes is significantly higher than the proportion of demethylation genes. The methylation level of reciprocal cross F1 generation is lower than the male diploid and higher than the female tetraploid. The hemimethylation and total methylation rate of the cross hybrid F1 generation is significantly higher than the orthogonal F1 generation (p < 0.01). After readjusting, the methylation pattern of genome DNA of reciprocal hybrids changed 69.59% and 72.83%, respectively. View Full-Text
Keywords: Misgurnus anguillicaudatus; diploid; triploid; tetraploid; parent–offspring generation; DNA methylation Misgurnus anguillicaudatus; diploid; triploid; tetraploid; parent–offspring generation; DNA methylation
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MDPI and ACS Style

Zhou, H.; Ma, T.-Y.; Zhang, R.; Xu, Q.-Z.; Shen, F.; Qin, Y.-J.; Xu, W.; Wang, Y.; Li, Y.-J. Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus. Int. J. Mol. Sci. 2016, 17, 1299. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081299

AMA Style

Zhou H, Ma T-Y, Zhang R, Xu Q-Z, Shen F, Qin Y-J, Xu W, Wang Y, Li Y-J. Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus. International Journal of Molecular Sciences. 2016; 17(8):1299. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081299

Chicago/Turabian Style

Zhou, He, Tian-Yu Ma, Rui Zhang, Qi-Zheng Xu, Fu Shen, Yan-Jie Qin, Wen Xu, Yuan Wang, and Ya-Juan Li. 2016. "Analysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus" International Journal of Molecular Sciences 17, no. 8: 1299. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms17081299

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