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Article

Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine

1
Equipe d’Accueil 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), Pôle Santé, UFR d’Odontologie, SFR-CAP Santé (FED 4231), Université de Reims Champagne-Ardenne, 1 Avenue du Maréchal Juin, 51100 Reims, France
2
UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100 Reims, France
3
UFR d’Odontologie, Université de Reims Champagne-Ardenne, 51100 Reims, France
4
Pole Pharmacie Pharmacovigilance CHU Reims, 51100 Reims, France
5
Laboratoire d’Anatomie et Cytologie Pathologiques, Centre Hospitalier-Universitaire, Hôpital Robert Debré, 51100 Reims, France
6
CNRS, UMR 7369, Medyc, Université de Reims Champagne-Ardenne, 51100 Reims, France
7
Equipe d’Accueil A 3795, Groupe d’Étude des Géomatériaux et Environnement Naturels, Anthropiques et Archéologiques (GEGENAA), Université de Reims Champagne Ardenne, 51100 Reims, France
8
CNRS, UMR 7563, LEMTA, Université de Lorraine, 54500 Vandœuvre-Lès-Nancy, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work
Int. J. Mol. Sci. 2017, 18(10), 2210; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102210
Received: 26 September 2017 / Revised: 14 October 2017 / Accepted: 18 October 2017 / Published: 21 October 2017
(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering 2018)
Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE®, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE® foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton’s jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications. View Full-Text
Keywords: stem cell niche; medical device; regenerative medicine; biocompatibility; paracrine activities stem cell niche; medical device; regenerative medicine; biocompatibility; paracrine activities
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MDPI and ACS Style

Aubert, L.; Dubus, M.; Rammal, H.; Bour, C.; Mongaret, C.; Boulagnon-Rombi, C.; Garnotel, R.; Schneider, C.; Rahouadj, R.; Laurent, C.; Gangloff, S.C.; Velard, F.; Mauprivez, C.; Kerdjoudj, H. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine. Int. J. Mol. Sci. 2017, 18, 2210. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102210

AMA Style

Aubert L, Dubus M, Rammal H, Bour C, Mongaret C, Boulagnon-Rombi C, Garnotel R, Schneider C, Rahouadj R, Laurent C, Gangloff SC, Velard F, Mauprivez C, Kerdjoudj H. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine. International Journal of Molecular Sciences. 2017; 18(10):2210. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102210

Chicago/Turabian Style

Aubert, Léa, Marie Dubus, Hassan Rammal, Camille Bour, Céline Mongaret, Camille Boulagnon-Rombi, Roselyne Garnotel, Céline Schneider, Rachid Rahouadj, Cedric Laurent, Sophie C. Gangloff, Frédéric Velard, Cedric Mauprivez, and Halima Kerdjoudj. 2017. "Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine" International Journal of Molecular Sciences 18, no. 10: 2210. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102210

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