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Article

Differential Gene Expression in the Meristem and during Early Fruit Growth of Pisum sativum L. Identifies Potential Targets for Breeding

1
School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand
2
School of Life Sciences, Yantai University, Yantai 264005, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Int. J. Mol. Sci. 2017, 18(2), 428; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020428
Received: 4 October 2016 / Revised: 15 December 2016 / Accepted: 8 February 2017 / Published: 16 February 2017
(This article belongs to the Special Issue Pulses)
For successful molecular breeding it is important to identify targets to the gene family level, and in the specific species of interest, in this case Pisum sativum L. The cytokinins have been identified as a key breeding target due to their influence on plant architecture, and on seed size and sink activity. We focused on the cytokinin biosynthetic gene family (the IPTs) and the gene family key to the destruction of cytokinins (the CKXs), as well as other gene families potentially affected by changing cytokinin levels. These included key meristem genes (WUS and BAM1) and the transporter gene families, sucrose transporters (SUTs) and amino acid permeases (AAPs). We used reverse transcription quantitative PCR (RT-qPCR) to monitor gene expression in the vegetative meristem and in pre- and post-fertilisation young pea fruits. PsWUS expression was specific to the shoot apical meristem while PsBAM1 was highly expressed in the shoot apical meristem (SAM) but was also expressed at a low level in the young fruit. Differential expression was shown between genes and within gene families for IPT, CKX, SUT, and AAP. PsCKX7 showed strong gene family member-specific expression in the SAM, and was also expressed in young pea fruits. We suggest that PsCKX7 is a potential target for downregulation via molecular breeding or gene editing. View Full-Text
Keywords: cytokinin; isopentenyl transferase; cytokinin oxidase/dehydrogenase; sucrose transporter; amino acid permease; WUSCHEL; BAM1; process pea; field pea; gene editing cytokinin; isopentenyl transferase; cytokinin oxidase/dehydrogenase; sucrose transporter; amino acid permease; WUSCHEL; BAM1; process pea; field pea; gene editing
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MDPI and ACS Style

Smitha Ninan, A.; Shah, A.; Song, J.; Jameson, P.E. Differential Gene Expression in the Meristem and during Early Fruit Growth of Pisum sativum L. Identifies Potential Targets for Breeding. Int. J. Mol. Sci. 2017, 18, 428. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020428

AMA Style

Smitha Ninan A, Shah A, Song J, Jameson PE. Differential Gene Expression in the Meristem and during Early Fruit Growth of Pisum sativum L. Identifies Potential Targets for Breeding. International Journal of Molecular Sciences. 2017; 18(2):428. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020428

Chicago/Turabian Style

Smitha Ninan, Annu, Anish Shah, Jiancheng Song, and Paula E. Jameson 2017. "Differential Gene Expression in the Meristem and during Early Fruit Growth of Pisum sativum L. Identifies Potential Targets for Breeding" International Journal of Molecular Sciences 18, no. 2: 428. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020428

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