Next Article in Journal
Bioinformatics Approaches for Fetal DNA Fraction Estimation in Noninvasive Prenatal Testing
Next Article in Special Issue
Vanillin Suppresses Cell Motility by Inhibiting STAT3-Mediated HIF-1α mRNA Expression in Malignant Melanoma Cells
Previous Article in Journal
High-Frequency Repetitive Transcranial Magnetic Stimulation (rTMS) Improves Functional Recovery by Enhancing Neurogenesis and Activating BDNF/TrkB Signaling in Ischemic Rats
Previous Article in Special Issue
Hazelnut (Corylus avellana L.) Shells Extract: Phenolic Composition, Antioxidant Effect and Cytotoxic Activity on Human Cancer Cell Lines
Article

Quantitative Determination of Stilbenoids and Dihydroisocoumarins in Shorea roxburghii and Evaluation of Their Hepatoprotective Activity

1
Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan
2
Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan
3
Faculty of Agro-Industry, Rajamangala University of Technology Srivijaya, Thungyai, Nakhon Si Thammarat 80240, Thailand
4
Faculty of Science and Technology, Rajamangala University of Technology Srivijaya, Thungyai, Nakhon Si Thammarat 80240, Thailand
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Battino
Int. J. Mol. Sci. 2017, 18(2), 451; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020451
Received: 28 December 2016 / Revised: 13 February 2017 / Accepted: 15 February 2017 / Published: 20 February 2017
(This article belongs to the Special Issue Biological Activity of Natural Secondary Metabolite Products)
A simultaneous quantitative analytical method for 13 stilbenoids including (−)-hopeaphenol (1), (+)-isohopeaphenol (2), hemsleyanol D (3), (−)-ampelopsin H (4), vaticanols A (5), E (6), and G (7), (+)-α-viniferin (8), pauciflorol A (9), hopeafuran (10), (−)-balanocarpol (11), (−)-ampelopsin A (12), and trans-resveratrol 10-C-β-d-glucopyranoside (13), and two dihydroisocoumarins, phayomphenols A1 (14) and A2 (15) in the extract of Shorea roxburghii (dipterocarpaceae) was developed. According to the established protocol, distributions of these 15 polyphenols (115) in the bark and wood parts of S. roxburghii and a related plant Cotylelobium melanoxylon were evaluated. In addition, the principal polyphenols (1, 2, 8, 1315) exhibited hepatoprotective effects against d-galactosamine (d-galN)/lipopolysaccharide (LPS)-induced liver injury in mice at a dose of 100 or 200 mg/kg, p.o. To characterize the mechanisms of action, the isolates were examined in in vitro studies assessing their effects on (i) d-GalN-induced cytotoxicity in primary cultured mouse hepatocytes; (ii) LPS-induced nitric oxide (NO) production in mouse peritoneal macrophages; and (iii) tumor necrosis factor-α (TNF-α)-induced cytotoxicity in L929 cells. The mechanisms of action of these polyphenols (1, 2, and 8) were suggested to be dependent on the inhibition of LPS-induced macrophage activation and reduction of sensitivity of hepatocytes to TNF-α. However, none of the isolates reduced the cytotoxicity caused by d-GalN. View Full-Text
Keywords: Shorea roxburghii; stilbenoid; dihydroisocoumarin; quantitative analysis; hepatoprotective effect; mechanism of action Shorea roxburghii; stilbenoid; dihydroisocoumarin; quantitative analysis; hepatoprotective effect; mechanism of action
Show Figures

Graphical abstract

MDPI and ACS Style

Ninomiya, K.; Chaipech, S.; Kunikata, Y.; Yagi, R.; Pongpiriyadacha, Y.; Muraoka, O.; Morikawa, T. Quantitative Determination of Stilbenoids and Dihydroisocoumarins in Shorea roxburghii and Evaluation of Their Hepatoprotective Activity. Int. J. Mol. Sci. 2017, 18, 451. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020451

AMA Style

Ninomiya K, Chaipech S, Kunikata Y, Yagi R, Pongpiriyadacha Y, Muraoka O, Morikawa T. Quantitative Determination of Stilbenoids and Dihydroisocoumarins in Shorea roxburghii and Evaluation of Their Hepatoprotective Activity. International Journal of Molecular Sciences. 2017; 18(2):451. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020451

Chicago/Turabian Style

Ninomiya, Kiyofumi, Saowanee Chaipech, Yusuke Kunikata, Ryohei Yagi, Yutana Pongpiriyadacha, Osamu Muraoka, and Toshio Morikawa. 2017. "Quantitative Determination of Stilbenoids and Dihydroisocoumarins in Shorea roxburghii and Evaluation of Their Hepatoprotective Activity" International Journal of Molecular Sciences 18, no. 2: 451. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18020451

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop