Next Article in Journal
Combined Effects of Curcumin and Lycopene or Bixin in Yoghurt on Inhibition of LDL Oxidation and Increases in HDL and Paraoxonase Levels in Streptozotocin-Diabetic Rats
Next Article in Special Issue
Analysis of DNA Methylation Status in Bodily Fluids for Early Detection of Cancer
Previous Article in Journal
A Combination of Soybean and Haematococcus Extract Alleviates Ultraviolet B-Induced Photoaging
Previous Article in Special Issue
Perindopril Induces TSP-1 Expression in Hypertensive Patients with Endothelial Dysfunction in Chronic Treatment
Article

Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers

1
Clinical Laboratory, Faculdade de Medicina do ABC (FMABC), Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil
2
Biological Science Department, UNIFESP, Rua Prof. Artur Riedel, 275, CEP 09972-270 Diadema, SP, Brazil
3
Epidemiology Laboratory and Data Analysis, FMABC, Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: William Chi-shing Cho
Int. J. Mol. Sci. 2017, 18(4), 170; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18040170
Received: 27 September 2016 / Revised: 26 December 2016 / Accepted: 8 January 2017 / Published: 23 March 2017
(This article belongs to the Special Issue Liquid Biopsy for Clinical Application)
Background: Patients with breast cancer—the deadliest cancer among women—are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains relatively normal, or even more alkaline due to the action of a membrane protein family known as monocarboxylate transporters (MCTs). The objective of this study was to verify the diagnostic and prognostic value of MCT1, MCT4 and CD147 in tumor and peripheral blood samples of patients with breast cancer undergoing chemotherapic treatment. Methods: Differential expression of MCT1, MCT4 and CD147 obtained by qPCR was determined by 2−ΔΔCq method between biological samples (tumor and serial samples of peripheral) of patients (n = 125) and healthy women (n = 25). Results: tumor samples with higher histological grades have shown higher expression of these markers; this higher expression was also observed in blood samples obtained at diagnosis of patients when compared to healthy women and in patients with positive progression of the disease (metastasis development). Conclusion: markers studied here could be a promising strategy in routine laboratory evaluations as breast cancer diagnosis and prognosis. View Full-Text
Keywords: biomarkers; prognosis; metastasis; breast cancer; monocarboxylate transporter (MCT); hypoxia biomarkers; prognosis; metastasis; breast cancer; monocarboxylate transporter (MCT); hypoxia
Show Figures

Figure 1

MDPI and ACS Style

Luz, M.C.d.B.; Perez, M.M.; Azzalis, L.A.; Sousa, L.V.d.A.; Adami, F.; Fonseca, F.L.A.; Alves, B.D.C.A. Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers. Int. J. Mol. Sci. 2017, 18, 170. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18040170

AMA Style

Luz MCdB, Perez MM, Azzalis LA, Sousa LVdA, Adami F, Fonseca FLA, Alves BDCA. Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers. International Journal of Molecular Sciences. 2017; 18(4):170. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18040170

Chicago/Turabian Style

Luz, Maria C.d.B., Matheus M. Perez, Ligia A. Azzalis, Luiz V.d.A. Sousa, Fernando Adami, Fernando L.A. Fonseca, and Beatriz D.C.A. Alves 2017. "Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers" International Journal of Molecular Sciences 18, no. 4: 170. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18040170

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop