Next Article in Journal
Fortifying Horticultural Crops with Essential Amino Acids: A Review
Next Article in Special Issue
A Novel Workflow to Enrich and Isolate Patient-Matched EpCAMhigh and EpCAMlow/negative CTCs Enables the Comparative Characterization of the PIK3CA Status in Metastatic Breast Cancer
Previous Article in Journal
Using Coexpression Protein Interaction Network Analysis to Identify Mechanisms of Danshensu Affecting Patients with Coronary Heart Disease
Previous Article in Special Issue
Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities
Article

The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young’s Modulus of Single Cells

by 1,2,†, 3,†, 1,2, 3, 1,2, 4, 5, 1,2,*, 5,6,* and 1,2,*
1
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
2
School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100190, China
3
Institute of Microelectronics of Chinese Academy of Sciences, Beijing 100029, China
4
Department of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA
5
Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33302, Taiwan
6
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City 33302, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Tuhin Subhra Santra and Fan-Gang Tseng
Int. J. Mol. Sci. 2017, 18(6), 1158; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061158
Received: 16 April 2017 / Revised: 23 May 2017 / Accepted: 25 May 2017 / Published: 19 June 2017
(This article belongs to the Special Issue Single Cell Technology)
This paper presents the instrumentation of a microfluidic analyzer enabling the characterization of single-cell biophysical properties, which includes seven key components: a microfluidic module, a pressure module, an imaging module, an impedance module, two LabVIEW platforms for instrument operation and raw data processing, respectively, and a Python code for data translation. Under the control of the LabVIEW platform for instrument operation, the pressure module flushes single cells into the microfluidic module with raw biophysical parameters sampled by the imaging and impedance modules and processed by the LabVIEW platform for raw data processing, which were further translated into intrinsic cellular biophysical parameters using the code developed in Python. Based on this system, specific membrane capacitance, cytoplasm conductivity, and instantaneous Young’s modulus of three cell types were quantified as 2.76 ± 0.57 μF/cm2, 1.00 ± 0.14 S/m, and 3.79 ± 1.11 kPa for A549 cells (ncell = 202); 1.88 ± 0.31 μF/cm2, 1.05 ± 0.16 S/m, and 3.74 ± 0.75 kPa for 95D cells (ncell = 257); 2.11 ± 0.38 μF/cm2, 0.87 ± 0.11 S/m, and 5.39 ± 0.89 kPa for H460 cells (ncell = 246). As a semi-automatic instrument with a throughput of roughly 1 cell per second, this prototype instrument can be potentially used for the characterization of cellular biophysical properties. View Full-Text
Keywords: instrumentation; microfluidics; single-cell analysis; specific membrane capacitance; cytoplasm conductivity; instantaneous Young’s modulus instrumentation; microfluidics; single-cell analysis; specific membrane capacitance; cytoplasm conductivity; instantaneous Young’s modulus
Show Figures

Figure 1

MDPI and ACS Style

Wang, K.; Zhao, Y.; Chen, D.; Huang, C.; Fan, B.; Long, R.; Hsieh, C.-H.; Wang, J.; Wu, M.-H.; Chen, J. The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young’s Modulus of Single Cells. Int. J. Mol. Sci. 2017, 18, 1158. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061158

AMA Style

Wang K, Zhao Y, Chen D, Huang C, Fan B, Long R, Hsieh C-H, Wang J, Wu M-H, Chen J. The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young’s Modulus of Single Cells. International Journal of Molecular Sciences. 2017; 18(6):1158. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061158

Chicago/Turabian Style

Wang, Ke, Yang Zhao, Deyong Chen, Chengjun Huang, Beiyuan Fan, Rong Long, Chia-Hsun Hsieh, Junbo Wang, Min-Hsien Wu, and Jian Chen. 2017. "The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young’s Modulus of Single Cells" International Journal of Molecular Sciences 18, no. 6: 1158. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061158

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop