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The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence

Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin 300071, China
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(6), 1303; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061303
Received: 13 May 2017 / Revised: 12 June 2017 / Accepted: 13 June 2017 / Published: 21 June 2017
(This article belongs to the Section Molecular Plant Sciences)
Genes essential for gametophyte development and fertilization have been identified and studied in detail; however, genes that fine-tune these processes are largely unknown. Here, we characterized an unknown Arabidopsis gene, GTP-BINDING PROTEIN RELATED1 (GPR1). GPR1 is specifically expressed in ovule, pollen, and pollen tube. Enhanced green fluorescent protein-tagged GPR1 localizes to both nucleus and cytoplasm, and it also presents in punctate and ring-like structures. gpr1 mutants exhibit no defect in gametogenesis and seed setting, except that their pollen grains are pale in color. Scanning electron microscopy analyses revealed a normal patterned but thinner exine on gpr1 pollen surface. This may explain why gpr1 pollen grains are pale. We next examined whether GPR1 mutation affects post gametogenesis processes including pollen germination, pollen tube growth, and ovule senescence. We found that gpr1 pollen grains germinated earlier, and their pollen tubes elongated faster. Emasculation assay revealed that unfertilized gpr1 pistil expressed the senescence marker PBFN1:GUS (GUS: a reporter gene that encodes β-glucuronidase) one-day earlier than the wild type pistil. Consistently, ovules and pollen grains of gpr1 mutants showed lower viability than those of the wild type at 4 to 5 days post anthesis. Together, these data suggest that GPR1 functions as a negative regulator of pollen germination, pollen tube growth, and gametophyte senescence to fine-tune the fertilization process. View Full-Text
Keywords: pollen germination; pollen tube; gametophyte; senescence; expression; pollen exine pollen germination; pollen tube; gametophyte; senescence; expression; pollen exine
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MDPI and ACS Style

Yang, X.; Zhang, Q.; Zhao, K.; Luo, Q.; Bao, S.; Liu, H.; Men, S. The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence. Int. J. Mol. Sci. 2017, 18, 1303. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061303

AMA Style

Yang X, Zhang Q, Zhao K, Luo Q, Bao S, Liu H, Men S. The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence. International Journal of Molecular Sciences. 2017; 18(6):1303. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061303

Chicago/Turabian Style

Yang, Xiao; Zhang, Qinying; Zhao, Kun; Luo, Qiong; Bao, Shuguang; Liu, Huabin; Men, Shuzhen. 2017. "The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence" Int. J. Mol. Sci. 18, no. 6: 1303. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061303

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