Next Article in Journal
The Intersection of NGF/TrkA Signaling and Amyloid Precursor Protein Processing in Alzheimer’s Disease Neuropathology
Next Article in Special Issue
Vitamin D Supplementation Enhances C18(dihydro)ceramide Levels in Type 2 Diabetes Patients
Previous Article in Journal
Gene Expression Profiling Reveals Potential Players of Left-Right Asymmetry in Female Chicken Gonads
Previous Article in Special Issue
pH Dependent Antimicrobial Peptides and Proteins, Their Mechanisms of Action and Potential as Therapeutic Agents
Article

Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients

1
Department of Obstetrics and Gynaecology, Breast Center, Ludwig-Maximilians University of Munich (LMU), Maistrasse 11, Munich 80337, Germany
2
Faculty of Pharmacy, University Paul Sabatier Toulouse III, Toulouse cedex 09 31062, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(6), 1318; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061318
Received: 11 May 2017 / Revised: 14 June 2017 / Accepted: 16 June 2017 / Published: 20 June 2017
The Vitamin D receptor (VDR) expressed in normal breast tissue and breast tumors has been suggested as a new prognostic biomarker in breast cancer (BC). Besides, increasing evidence supports the view that the detection of circulating tumor cells (CTCs) predicts outcome in early and metastatic BC. Consequently, an evaluation of VDR expression in the CTCs of BC patients may allow optimization of their treatment. As an attempt to profile and subtype the CTCs of metastatic patients, we established an innovative fluorescence technique using nine BC cell lines to visualize, define, and compare their individual VDR status. Afterwards, we tested the CTC presence and VDR expression in blood samples (cytospins) collected from 23 metastatic BC patients. The results demonstrated major differences in the VDR levels among the nine cell lines, and VDR positive CTCs were detected in 46% of CTC-positive patients, with a total of 42 CTCs individually analyzed. Due to the limited number of patients in this study, no correlation between VDR expression and BC subtype classification (according to estrogen receptor (ER), progesterone receptor (PR) and HER2) could be determined, but our data support the view that VDR evaluation is a potential new prognostic biomarker to help in the optimization of therapy management for BC patients. View Full-Text
Keywords: vitamin D receptor; circulating tumor cells; breast cancer vitamin D receptor; circulating tumor cells; breast cancer
Show Figures

Graphical abstract

MDPI and ACS Style

Zhang, X.; Hofmann, S.; Rack, B.; Harbeck, N.; Jeschke, U.; Sixou, S. Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients. Int. J. Mol. Sci. 2017, 18, 1318. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061318

AMA Style

Zhang X, Hofmann S, Rack B, Harbeck N, Jeschke U, Sixou S. Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients. International Journal of Molecular Sciences. 2017; 18(6):1318. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061318

Chicago/Turabian Style

Zhang, Xi; Hofmann, Simone; Rack, Brigitte; Harbeck, Nadia; Jeschke, Udo; Sixou, Sophie. 2017. "Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients" Int. J. Mol. Sci. 18, no. 6: 1318. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18061318

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop