Next Article in Journal
Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors
Next Article in Special Issue
Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro
Previous Article in Journal
Shear Bond Strength and Remineralisation Effect of a Casein Phosphopeptide-Amorphous Calcium Phosphate-Modified Glass Ionomer Cement on Artificial “Caries-Affected” Dentine
Previous Article in Special Issue
Intrapancreatic Parenchymal Injection of Cells as a Useful Tool for Allowing a Small Number of Proliferative Cells to Grow In Vivo
Article

Effects of Co-Culture Media on Hepatic Differentiation of hiPSC with or without HUVEC Co-Culture

1
Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité—Universitätsmedizin Berlin, Campus Virchow-Klinikum, 13353 Berlin, Germany
2
Janssen Research and Development, 2340 Beerse, Belgium
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(8), 1724; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18081724
Received: 3 July 2017 / Revised: 24 July 2017 / Accepted: 2 August 2017 / Published: 7 August 2017
(This article belongs to the Special Issue Stem Cell Research)
The derivation of hepatocytes from human induced pluripotent stem cells (hiPSC) is of great interest for applications in pharmacological research. However, full maturation of hiPSC-derived hepatocytes has not yet been achieved in vitro. To improve hepatic differentiation, co-cultivation of hiPSC with human umbilical vein endothelial cells (HUVEC) during hepatic differentiation was investigated in this study. In the first step, different culture media variations based on hepatocyte culture medium (HCM) were tested in HUVEC mono-cultures to establish a suitable culture medium for co-culture experiments. Based on the results, two media variants were selected to differentiate hiPSC-derived definitive endodermal (DE) cells into mature hepatocytes with or without HUVEC addition. DE cells differentiated in mono-cultures in the presence of those media variants showed a significant increase (p < 0.05) in secretion of α-fetoprotein and in activities of cytochrome P450 (CYP) isoenzymes CYP2B6 and CYP3A4 as compared with cells differentiated in unmodified HCM used as control. Co-cultivation with HUVEC did not further improve the differentiation outcome. Thus, it can be concluded that the effect of the used medium outweighed the effect of HUVEC co-culture, emphasizing the importance of the culture medium composition for hiPSC differentiation. View Full-Text
Keywords: human induced pluripotent stem cells (hiPSC); hepatic differentiation; human umbilical vein endothelial cells (HUVEC); co-culture human induced pluripotent stem cells (hiPSC); hepatic differentiation; human umbilical vein endothelial cells (HUVEC); co-culture
Show Figures

Graphical abstract

MDPI and ACS Style

Freyer, N.; Greuel, S.; Knöspel, F.; Strahl, N.; Amini, L.; Jacobs, F.; Monshouwer, M.; Zeilinger, K. Effects of Co-Culture Media on Hepatic Differentiation of hiPSC with or without HUVEC Co-Culture. Int. J. Mol. Sci. 2017, 18, 1724. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18081724

AMA Style

Freyer N, Greuel S, Knöspel F, Strahl N, Amini L, Jacobs F, Monshouwer M, Zeilinger K. Effects of Co-Culture Media on Hepatic Differentiation of hiPSC with or without HUVEC Co-Culture. International Journal of Molecular Sciences. 2017; 18(8):1724. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18081724

Chicago/Turabian Style

Freyer, Nora, Selina Greuel, Fanny Knöspel, Nadja Strahl, Leila Amini, Frank Jacobs, Mario Monshouwer, and Katrin Zeilinger. 2017. "Effects of Co-Culture Media on Hepatic Differentiation of hiPSC with or without HUVEC Co-Culture" International Journal of Molecular Sciences 18, no. 8: 1724. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18081724

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop