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Recent Advances in Experimental Whole Genome Haplotyping Methods

by , and *
State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2017, 18(9), 1944; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18091944
Received: 3 August 2017 / Revised: 1 September 2017 / Accepted: 5 September 2017 / Published: 11 September 2017
(This article belongs to the Special Issue Single Cell Technology)
Haplotype plays a vital role in diverse fields; however, the sequencing technologies cannot resolve haplotype directly. Pioneers demonstrated several approaches to resolve haplotype in the early years, which was extensively reviewed. Since then, numerous methods have been developed recently that have significantly improved phasing performance. Here, we review experimental methods that have emerged mainly over the past five years, and categorize them into five classes according to their maximum scale of contiguity: (i) encapsulation, (ii) 3D structure capture and construction, (iii) compartmentalization, (iv) fluorography, (v) long-read sequencing. Several subsections of certain methods are attached to each class as instances. We also discuss the relative advantages and disadvantages of different classes and make comparisons among representative methods of each class. View Full-Text
Keywords: haplotype; haplotyping; phasing; next generation sequencing haplotype; haplotyping; phasing; next generation sequencing
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MDPI and ACS Style

Huang, M.; Tu, J.; Lu, Z. Recent Advances in Experimental Whole Genome Haplotyping Methods. Int. J. Mol. Sci. 2017, 18, 1944. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18091944

AMA Style

Huang M, Tu J, Lu Z. Recent Advances in Experimental Whole Genome Haplotyping Methods. International Journal of Molecular Sciences. 2017; 18(9):1944. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18091944

Chicago/Turabian Style

Huang, Mengting, Jing Tu, and Zuhong Lu. 2017. "Recent Advances in Experimental Whole Genome Haplotyping Methods" International Journal of Molecular Sciences 18, no. 9: 1944. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18091944

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