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Article

Nucleic Acid-Dependent Structural Transition of the Intrinsically Disordered N-Terminal Appended Domain of Human Lysyl-tRNA Synthetase

Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea
*
Author to whom correspondence should be addressed.
These authors equally contributed to this manuscript.
Int. J. Mol. Sci. 2018, 19(10), 3016; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19103016
Received: 27 August 2018 / Revised: 27 September 2018 / Accepted: 29 September 2018 / Published: 3 October 2018
(This article belongs to the Collection Protein Folding)
Eukaryotic lysyl-tRNA synthetases (LysRS) have an N-terminal appended tRNA-interaction domain (RID) that is absent in their prokaryotic counterparts. This domain is intrinsically disordered and lacks stable structures. The disorder-to-order transition is induced by tRNA binding and has implications on folding and subsequent assembly into multi-tRNA synthetase complexes. Here, we expressed and purified RID from human LysRS (hRID) in Escherichia coli and performed a detailed mutagenesis of the appended domain. hRID was co-purified with nucleic acids during Ni-affinity purification, and cumulative mutations on critical amino acid residues abolished RNA binding. Furthermore, we identified a structural ensemble between disordered and helical structures in non-RNA-binding mutants and an equilibrium shift for wild-type into the helical conformation upon RNA binding. Since mutations that disrupted RNA binding led to an increase in non-functional soluble aggregates, a stabilized RNA-mediated structural transition of the N-terminal appended domain may have implications on the functional organization of human LysRS and multi-tRNA synthetase complexes in vivo. View Full-Text
Keywords: aminoacyl-tRNA synthetase; LysRS; hRID; disorder-helix transition aminoacyl-tRNA synthetase; LysRS; hRID; disorder-helix transition
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MDPI and ACS Style

Kwon, S.B.; Yu, J.E.; Park, C.; Lee, J.; Seong, B.L. Nucleic Acid-Dependent Structural Transition of the Intrinsically Disordered N-Terminal Appended Domain of Human Lysyl-tRNA Synthetase. Int. J. Mol. Sci. 2018, 19, 3016. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19103016

AMA Style

Kwon SB, Yu JE, Park C, Lee J, Seong BL. Nucleic Acid-Dependent Structural Transition of the Intrinsically Disordered N-Terminal Appended Domain of Human Lysyl-tRNA Synthetase. International Journal of Molecular Sciences. 2018; 19(10):3016. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19103016

Chicago/Turabian Style

Kwon, Soon B., Ji E. Yu, Chan Park, Jiseop Lee, and Baik L. Seong 2018. "Nucleic Acid-Dependent Structural Transition of the Intrinsically Disordered N-Terminal Appended Domain of Human Lysyl-tRNA Synthetase" International Journal of Molecular Sciences 19, no. 10: 3016. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19103016

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