Differential Effect of Cobalt and Chromium Ions as Well as CoCr Particles on the Expression of Osteogenic Markers and Osteoblast Function

Abstract

The balance of bone formation and resorption is the result of a regulated crosstalk between osteoblasts, osteoclasts, and osteocytes. Inflammation, mechanical load, and external stimuli modulate this system. Exposure of bone cells to metal ions or wear particles are thought to cause osteolysis via activation of osteoclasts and inhibition of osteoblast activity. Co²⁺ ions have been shown to impair osteoblast function and the expression of the three transforming growth factor (TGF)-β isoforms. The current study was performed to analyze how Co²⁺ and Cr³⁺ influence the expression, proliferation, and migration profile of osteoblast-like cells. The influence of Co²⁺, Cr³⁺, and CoCr particles on gene expression was analyzed using an osteogenesis PCR Array. The expression of different members of the TGF-β signaling cascade were down-regulated by Co²⁺, as well as several TGF-β regulated collagens, however, Cr³⁺ had no effect. CoCr particles partially affected similar genes as the Co²⁺treatment. Total collagen production of Co²⁺ treated osteoblasts was reduced, which can be explained by the reduced expression levels of various collagens. While proliferation of MG63 cells appears unaffected by Co²⁺, the migration capacity was impaired. Our data may improve the knowledge of changes in gene expression patterns, and the proliferation and migration effects caused by artificial materials.

1. Introduction

Total hip arthroplasty (THA) is an effective and safe technique to treat degenerative, post-traumatic, and other diseases of the hip joint. One of the main reasons for a limited lifespan of THAs is aseptic loosening due to increased bone resorption [1,2]. The tight regulation of bone formation and resorption is therefore of great importance to ensure the THA function. Osteoblast activity is important during implant ingrowth and the prevention of implant loosening, which requires mature osteoblasts to deposit bone with remarkable spatial precision.

Many metal implant devices used in orthopaedic surgery are manufactured from cobalt and chromium alloys. Metal particles can be degraded into their respective ions in periprosthetic tissue [3,4,5]. Abrasive wear particles liberated from the articulation surfaces or cone-taper connections of metal-containing implants can induce adverse biological reactions in periprosthetic tissue as well as systemic effects caused by released metal ions (Co²⁺/Cr³⁺) and metal degradation products (e.g., oxides, insoluble metal salts, and metal-protein complexes) [6,7,8]. Particles and metal ions affect the cells of the surrounding tissues, including immune cells (lymphocytes, macrophages), bone cells (osteoblasts, osteoclasts), and fibroblasts [9]. These abrasive wear particles are thought to induce osteolysis by shifting the sensitive balance of bone homeostasis towards bone resorption or reduced osteoblast activity. The exact mechanisms of this interaction are only partially understood.

Bone homeostasis is tightly regulated by growth factors, cytokines, hormones, mechanical load, and other variables. Among these factors, transforming growth factor (TGF)-β and bone marrow proteins (BMPs) play an important role. BMP morphogen gradients regulate bone formation during embryogenesis [10]. Disruptions of TGF-β/BMP signaling have been implicated in multiple bone diseases including tumor metastasis [11]. It is known that TGF-β activates COL1A1 synthesis thereby providing the matrix for mineralization and bone formation [12,13]. Bone tissue is mainly composed of type I collagen, which is also thought to be a marker for osteoblast activity [14]. However, other collagens are also expressed in bone tissue to a smaller amount contributing either to the mechanical properties of the bone or regulating bioavailability of morphogens and cytokines. One of these collagens is type III collagen (Col3), which can be found in heterotypic fibrils with type I collagen (Col1) [15,16,17]. Other non-fibrilar collagens are also found in bone tissue, e.g., collagen XIV, which is found mainly in tissues containing type I collagen [18], and collagen XV which participates in extracellular matrix (ECM) organization in the early-phases of the osteogenic process and that is a prerequisite to promote the subsequent deposition of the mineral matrix [19]. The osteoblast, as the bone forming and remodelling cell, produces a variety of cytokines and chemokines, both under physiologic and pathological conditions regulating the osteoblast migration to the exact site of bone formation [20,21]. Alrabeah and co-workers demonstrated that Co²⁺ metal ions in the culture media induced the production of pro-inflammatory cytokines in human osteoblastic cells, thereby inducing a shift of bone homeostasis towards bone resorption [22]. Furthermore, it is known that Co²⁺ ions reduce the expression of all three TGF-β isoforms in osteosarcoma cell lines MG63 and SaOs-2 in a dose-dependent manner, with the strongest impact on TGF-β2 resulting in a change of the TGF-β isoform pattern [23]. It is still unknown whether these changes in the expression pattern of TGF-β isoforms are involved in the dysregulation of the periprosthetic bone metabolism and function of osteoblasts.

It was the aim of the study to investigate the effect of Co²⁺ and Cr³⁺ ions on signaling pathways of bone metabolism and osteoblast functions. We hypothesized that Co²⁺ and Cr³⁺ ions have different influence on cell migration and the expression of osteoblast related genes that may induce bone loss. Therefore, we used the osteoblast-like cell line MG63, stimulated these cells with Co²⁺ and Cr³⁺ ions (0–250 µM) as well as CoCr particles, and analyzed the migration properties and gene expression on a low density Profiler PCR Array.

2. Results

2.1. Cell Proliferation

Proliferation of osteoblasts was determined by quantification of Bromodeoxyuridine (BrdU) incorporation. As shown in Figure 1, all Co²⁺ concentrations induced a slight, but not significant, increase in proliferation activity for MG63 as well as SaOs-2 cells.

Additionally, for Cr³⁺ treatment, no effects on cell proliferation were observed for any ion concentration. To further validate this result, we investigated the expression of the proliferation marker proliferating cell nuclear antigen (PCNA) using quantitative RT-PCR in MG63 cells. We observed no change in PCNA expression at a sub-confluent concentration of 1 × 10⁵ cells upon stimulation with Co²⁺ in a concentration range between 0–250 µM

2.2. Cell Migration

The influence of Co²⁺ and Cr³⁺ ions and CoCr particles on the migration capability of MG63 and SaOs-2 cells was analyzed using a wound healing assay. In MG63 cells, the standardized gap was reduced by 50 ± 11.3%, and after 48 h, a complete closure of the gap was seen. Co²⁺ treatment of cells resulted in a deceleration of this process, even after 48 h, the gap was still open. In contrast, Cr³⁺ accelerated this process in comparison to the unstimulated control. After 24 h, the gap was reduced by 67.0 ± 15.1% and completely closed after 48 h. Representative images for Co²⁺ and Cr³⁺ treated MG63 cells are shown in Figure 2A. The treatment of cells with CoCr particles (1 × 10⁶/well) had no significant effect. The summary of the wound healing assay in MG63 and SaOs-2 cells are summarized in Figure 2B,C.

2.3. Expression of Osteogenic Markers

Human osteoblast-like MG63 cells were incubated without stimulus (control), and with CoCl₂ (250 µM), CrCl₃ (250 µM), and CoCr-particles (1 × 10⁶/well) in 12-well plates for 24 h.

Fifteen out of 84 genes were found to be expressed at a low level (C_t > 35 cycles), which was considered as non-detected. After normalization of the gene target expression to glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 29 genes were found to be differentially regulated in CoCl₂ treated MG63 cells compared to the untreated control by at least a factor of 2. The majority of genes were down-regulated (n = 25) (

Table 1. Genes down-regulated by CoCl₂ after 24 h by at least a factor of 2 (normalization of C_t-values to glyceraldehyde 3-phosphate dehydrogenase (GAPDH)). The significance level was adjusted for multiple testing to 0.0006 (bold p-values are significant). A = in either the control or the test sample, the average C_t is high (>30 cycles).

Gene Symbol	Fold Regulation	Comments	p-Value
BGN	−2.12		3.45254 × 10−5
BMP4	−2.81		3.30439 × 10−5
BMP5	−21.52	A	1.83701 × 10−5
BMPR2	−2.15		0.003576291
CDH11	−4.14		7.55543 × 10−7
CHRD	−2.22		0.025290803
COL14A1	−6.54		0.000723157
COL15A1	−6.74	A	4.58304 × 10−7
COL1A1	−2.52		0.000370615
COL1A2	−3.23		7.20666 × 10−6
COL3A1	−10.78		1.96377 × 10−5
COL5A1	−2.03		0.000462367
COMP	−2.65		0.000687369
IGF1R	−2.85		0.001021934
ITGA1	−2.13		0.037993599
ITGA3	−2.12		0.018352525
MMP2	−2.7		6.30662 × 10−7
RUNX2	−2.51		3.16733 × 10−6
SMAD1	−2.93		1.23015 × 10−5
SMAD2	−2.06		0.002343501
SOX9	−3.18		2.62519 × 10−6
TGFB2	−7.4	A	4.4503 × 10−7
TGFB3	−2.27		8.28135 × 10−5
TWIST1	−2.32		0.00077027
VEGFB	−2.1		0.002973727

), whereas only four genes were found to be up-regulated (

Table 2. Genes up-regulated by CoCl₂ after 24 h by at least a factor of 2 (normalization of C_t-values to GAPDH). The significance level was adjusted for multiple testing to 0.0006 (bold p-values are significant), A = in either the control or the test sample, the average C_t is high (>30 cycles).

Gene Symbol	Fold Regulation	Comments	p-Value
BMP6	4		0.015432431
EGF	3.89		0.00777753
FGF1	2.11		0.010384109
PDGFA	3.15	A	0.000170489

).

The volcano plot (Figure 3) reflects the predominant down-regulation of transcription of osteogenic markers.

The strongest effect was seen on the expression of different collagens e.g., collagen 14A1 (−6.5-fold down-regulation, p = 0.0007), Col15A1 (−6.7-fold down-regulation, p = 4.58 × 10⁻⁷), and Col3A1 (−10.8-fold down-regulation, p = 1.96 × 10⁻⁵), but also Col1A1 (−2.5-fold down-regulation, p = 0.00037) and Col1A2 (−3.2-fold down-regulation, p = 7.20 × 10⁻⁵) as well as other extracellular matrix proteins, such as COMP (−2.65-fold down-regulation, p = 0.00068) and biglycan (−2.1-fold down-regulation, p = 3.45 × 10⁻⁵). Interestingly, different cytokines also involved in the regulation of bone homeostasis were decreased in expression e.g., members of the TGF-β family (TGF-β2 −7.4-fold, p = 4.5 × 10⁻⁵ and TGF-β3 −2.3-fold, p = 8.3 × 10⁻⁵) and BMPs (BMP4 −2.8-fold, p = 3.3 × 10⁻⁵ BMP5 −21.5-fold, p = 1.8 × 10⁻⁵). Co²⁺ ions also down-regulated the expression of downstream targets of the TGF-β family signaling cascade SMAD1 (−2.9-fold) and SMAD2 (−2.1-fold).

MG63 cells treated with Cr³⁺ did not show a significant change in the expression in any of the 84 genes tested.

CoCr particles at a concentration of 1 × 10⁶/well (3.83 cm²) affected the gene expression of several genes by at least a factor of 2 (Figure 4,

Table 3. Genes down-regulated after 24 h of stimulation with particles by at least a factor of 2 (normalization of C_t-values to GAPDH). The significance level was adjusted for multiple testing to 0.0006 (bold p-values are significant), A = in either the control or the test sample, the average C_t is high (>30 cycles).

Gene Symbol	Fold Regulation	Comments	p-Value
BMP5	−2.16		0.0008533
BMP6	−2.01		0.0209783
COL14A1	−2.01		0.0197225
COL15A1	−2.12	A	5.71 × 10−5
PDGFA	−2.13		0.0004219
SMAD1	−2.25		7.161 × 10−5
SMAD4	−2.18		0.1233691
VDR	−2.08		0.0001971

and

Table 4. Genes up-regulated after 24 h of stimulation with particles by at least a factor of 2 (normalization of C_t-values with GAPDH). At a significance level of 0.0006 (adjusted for multiple testing) no significant up-regulation was found, A = in either the control or the test sample, the average C_t is high (>30 cycles) B: the relative expression level is low (C_t > 30, in both control and test samples).

Gene Symbol	Fold Regulation	Comments	p-Value
FGF1	2.06	B	0.0666043
GDF10	3.15	B	0.0609019
NOG	2.39	A	0.0058095
VEGFA	2.17		0.0046092

), eight genes were down-regulated, four genes up-regulated. Interestingly, down-regulation of Col14A1 (−2.01-fold) and Col15A1 (−2.1-fold) was also seen, along with down-regulation of Co²⁺ treated cells, although to a lesser extent. Additionally, the down-regulation of BMP5 (−2.2-fold) was reproducible in particle treated cells.

2.4. Collagen Staining with Sirius Red

In line with the array data, we found that CoCl₂ reduced the total collagen production significantly in MG63 cells as shown by Sirius red staining. Figure 5 shows the dose-dependent decrease in collagen release upon Co²⁺ ion stimulation. As expected, no effect on collagen production was found in Cr³⁺ treated cells.

3. Discussion

Abrasive wear particles from endoprosthetic implants are known to induce adverse reactions in periprosthetic tissues (ARMD) such as osteolysis [24,25]. It has been shown that ARMD is associated with elevated serum levels of cobalt and chromium [25]. Cobalt measurements in the synovial fluid of failed metal on metal (M-o-M) hip replacements revealed a cobalt concentration of up to 30 µM [26]. Furthermore, a median concentration of 6.4 µg/g cobalt (range: 0.2–262 µg) within periprosthetic tissue was found around failed M-o-M hip implants [27]. For this reason, the Co²⁺ and Cr³⁺ ion concentrations were chosen in this manuscript. Co²⁺ and Cr³⁺ have been shown to modulate the expression of cytokines and chemokines in bone cells, inducing osteolytic processes or changes in osteoblast migration along chemokine gradients [9]. Interestingly, we have shown before that the C-X-C chemokine receptor type 4 (CXCR4) is up-regulated by different types of CoCr particles as well as dose-dependently by soluble Co²⁺ ions dose-dependently in osteoblast-like cells (MG63 and SaOs-2) [28,29]. CXCR4 is known to regulate osteoblast migration along chemokine gradients [30]. It has been shown that members of the TGF-β family are important cytokines involved in the maturation of osteoclasts and osteocytes [31], as well as regulation of bone formation by regulating extra cellular matrix deposition. In a previous study, we analyzed the influence of Co²⁺ and Cr³⁺ on the expression of the three TGF-β isoforms. After the binding of TGF-β to its cell surface, receptor Smad2 and Smad3 are phosphorylated and form complexes with Smad4 [32]. These complexes translocate to the nucleus and activate target genes including those required for assembly of the collagen extracellular matrix [32,33]. Our previous data show that bivalent cobalt ions and trivalent chromium ions have different effects on bone forming cells. While Co²⁺ down-regulated the expression of all three TGF-β isoforms in osteoblast-like cells, no inhibitory effect on mineralization was seen in the tested concentrations. Cr³⁺, however, did not influence the expression of TGF-β but strongly inhibited the mineralization in vitro [23]. These data suggest that the influence of Co²⁺ ions on bone homeostasis may be related to the inhibitory effect on the transcription of the bone formation regulating cytokines e.g., TGF-β1-3 and thereby on the bone forming activity of osteoblasts e.g., collagen production. The level of mineralization is determined by the expression of genes such as collagen, however, other post-transcriptional factors seem to have a stronger effect on mineralization.

One possible explanation for this effect might be that CoCl₂ initiates HIF-1α signaling [34]. HIF-1α has been shown to regulate fibrotic tissue changes by inducing collagen expression, as well as TGF-β signaling [35,36].

Cr³⁺ ions, however, did not influence the gene expression and therefore most likely interact with the mineralization process directly. Shah et al. showed comparable effects of cobalt and chromium on the mineralization of murine “osteoblast to osteocyte-like” cell line MLO-A5 [37]. The inhibition of mineralization by soluble Cr³⁺ or on soluble CrPO₄ might be due to the binding of phosphate to Cr³⁺, thereby reducing the availability of the ionic form [23]. However, this interaction needs to be analyzed in further studies.

To further investigate the underlying mechanisms we performed the RT² human osteogenesis PCR Profiler Array covering 84 genes involved in osteogenesis. Twenty-nine genes were found regulated in CoCl₂ treated MG63 cells compared to the untreated control by at least a factor of 2. These present data strengthen our recently published findings of a down-regulation of all TGF-β isoforms by Co²⁺, with the strongest effect on the expression of TGF-β2. Additionally, we observed that the expression of two other members of the TGF-β superfamily, BMP4 and BMP5, was significantly down-regulated by Co²⁺. Interestingly, BMP5 has been shown to up-regulate osteoclast formation, thereby inducing increased bone loss [38]. In contrast to Co²⁺/Cr³⁺-ions, CoCr particles showed no significant effect on migration and only a moderate effect on gene expression. There might be an effect after a prolonged exposure of osteoblasts to metal particles as a result of the intracellular corrosion, however, this was not investigated in this study.

The current study gives deeper insights into the mechanisms of how divalent Co²⁺ and trivalent Cr³⁺ alter bone homeostasis and contribute to osteolysis. Experiments using osteoclasts were beyond the scope of the study—nevertheless bone resorption caused by osteoclasts is a very important topic in bone homeostasis. Beside the canonical osteoclasts differentiation by receptor activator of NF-κB–receptor activator of NF-κB ligand (RANK–RANKL) interaction, Sabokbar et al. described a non-canonical, RANKL independent way of osteoclast differentiation [39]. The migration experiments showed that Co²⁺ (250 µM) decelerated migration whereas Cr³⁺ at the same concentration accelerates the migration rate compared to untreated MG63 cells. One reason for this effect could be changes in proliferation or metabolic activity. As shown previously the metabolic activity was slightly up-regulated by Co²⁺, whereas Cr³⁺ exhibited no effects on the cell metabolisms measured by water soluble tetrazolium (WST)-1 test [23]. Since metabolic activity determined using the WST-1 assay cannot be equated with proliferation, the proliferative activity of MG63 and SaOs-2 cells was measured after 48 h by determination of DNA replication using the BrdU assay—the results are similar to those of the WST-1 data, as well as the quantitative RT-PCR for the proliferation marker PCNA. These results are in agreement with the findings of other groups. Li and Wang reported that migration of smooth muscle cells is inhibited by Co²⁺ ions, whereas cell proliferation was only slightly increased [40].

As changes in proliferation of osteoblasts could not explain the decreased migratory capacity upon Co²⁺ ion stimulation, other pathways must be involved. The deposition of the extracellular matrix is important for cell migration. The extracellular matrix influences cellular migration, besides acting as a major reservoir of releasable chemokines it also provides guidance and confinement of the cell body resulting in a shape adaptation in order to move [41]. Therefore, the observed down-regulation of various extracellular matrix molecules, e.g., collagens and biglycan, could explain the reduced migratory capacity. The down-regulation of different collagen genes is in agreement with our observation that the production of total collagen is significantly reduced by CoCl₂. Interestingly, TGF-β has been shown to play a crucial role in the expression regulation of various collagens. TGF-β/Smad3 stimulation favored the secretion of collagen-3 versus collagen-1 [42]. A critical role for Col3 in skeletal development is suggested by its appearance in mesenchymal condensations preceding cartilage and bone formation [43], its requirement for growth acceleration of osteoblasts [44] thus making a role of Col3 in fracture healing or ingrowth of prosthesis possible [45].

Interestingly, TGF-β 1 significantly up-regulated collagen type XIV expression [46]. These changes in ECM production could explain the reduced migratory capacity of MG63 and SaOs-2 cells after incubation with Co²⁺ ions.

We are aware that our study is limited by several factors. The use of the osteogenic array for the analysis of the effects of Co²⁺ and Cr³⁺ on gene expression limits our findings to genes which are involved in functions of osteoblasts. As shown earlier, other genes are also affected [28,29]. Furthermore, instead of primary osteoblasts which exhibit large inter-individual variability as well as limited cell division, we used osteoblast-like cell lines for our experiments. MG63 and SaOs-2 cells do not have the limitations of primary cells but their tumor derived origin must be taken into account. The concentrations of Co²⁺ and Cr³⁺ used in this study are within a range described in literature [9] e.g., Mabilleau et al. used concentrations up to 100 µM and observed no cytotoxic effects [47], some other authors worked with even higher concentrations up to 370 µM for Co²⁺ and 2.8 mM for Cr³⁺ [48,49]. In previous studies, we tested all three concentrations and found no adverse effects in PCR experiments [23].

Besides contributing to the understanding of osteoblast functional pathways, our data contribute to the knowledge of changes in gene expression patterns induced by metallic implants and/or their corrosion products. These findings may also have a clinical impact and may support the development of so called “smart surfaces” that may recognize pathological conditions e.g., osteolysis in periprosthetic tissues or bacterial colonization on implant surfaces at early stages. Rebound strategies may be then introduced by eluting for example anti-inflammatory or antimicrobial drugs. All these interesting topics should be the focus of further studies.

4. Material and Methods

4.1. Cell Culture

MG63 and SaOs-2 osteoblast-like cells were cultured in Dulbecco’s modified eagle medium (DMEM), supplemented with 10% fetal calf serum (FCS), 1% penicillin/streptomycin at 37 °C, and in 5% CO₂ in a humidified atmosphere. For all experiments the cells were used from the 3th to the 7th passage.

Cell culture experiments for cell migration, RT² Profiler PCR Array, and experiments for collagen determination were performed in 12-well cell culture plates (growing area: 3.83 cm², final volume: 2 mL). For the determination of proliferation, 5 × 10³ cells were seeded into 96-well plates (growing area: 0.32 cm², final volume 100 µL). After overnight adherence of the cells, the medium was changed and the cells were treated with the given concentrations of cobalt(II)chloride and chromium(III)chloride, as well as with Co-35Ni-20Cr-10Mo particles. The MG63 and SaOs-2 cell lines are well-characterized [50,51]—they share properties with primary human osteoblasts. The response to parathyroid hormone is similar, as well as the inhibition of proliferation with 1,25-(OH)₂D₃, the 1,25-(OH)₂D₃ dependent increase of alkaline phosphatase activity and osteocalcin mRNA, and protein expression [52,53,54,55]. Therefore, MG63 and SaOs-2 cell lines can be considered as well-established models for osteoblast experiments and have been used in previous studies by us [28,29].

4.2. Preparation of CoCl₂ and CrCl₃ Solutions

For the application of Co²⁺ and Cr³⁺ ions in the cell culture experiments, 100 mM stock solutions were prepared. For the preparation of Co²⁺, CoCl₂ (Sigma Aldrich, Taufkirchen, Germany) was dissolved in water. For the preparation of Cr³⁺, CrCl₃ × 6 H₂O was dissolved in water. When this takes place, CrCl₃ × 6 H₂O forms as in initial species trans-[Cr³⁺(H₂O)₄Cl₂]Cl × 2 H₂O which appears emerald green. Within a time period of two weeks, this complex is reorganized to the final species [Cr³⁺(H₂O)₆]Cl₃ (purple)—this stable species was used for all chromium experiments.

4.3. Preparation of Particles

The wear particles of the Co-35Ni-20Cr-10Mo alloy (CoCr) were produced according to Buchhorn et al. [56] and as described previously [55]. Briefly, Co-35Ni-20Cr-10Mo containers were filled with bars of the same material and filled with absolute ethanol followed by eccentrical and continuous rotation at room temperature. By tumbling in this way, the bars rubbed against the wall of the container and generated the particles. The chemical composition and relative purity of the particles was validated by energy-dispersive X-ray (EDX) spectroscopy. The particle size spectra in the basic stock-suspension ranged from less than 0.1 µm to about 200 µm. More than 80% of all particles were smaller than 5 µm and the maximum size distribution was about 2 µm [57].

One milliliter of the metal suspension was applied on a suction filter using a 200 nm pore size polyester membrane (Steritech, Charlotte, NC, USA). The dried filters were applied to sterile glass vials containing 10 mL DMEM medium (without any supplements) and irradiated under UV-light overnight in a laminar flow. For the experiments, stock solutions of all particle types containing 1 × 10⁸ particles per mL were prepared—the highest concentration used in cell culture was 1 × 10⁶ particles/well. All particle solutions tested endotoxin free using the E-TOXATE test (Sigma-Aldrich, Taufkirchen, Germany).

4.4. Cell Viability

The viability of MG63 and SaOs-2 cells after stimulation with CoCl₂ and CrCl₃ was measured by Trypan blue exclusion test. In viable cells Trypan blue is not absorbed, however, it traverses the cells membranes in dead cells. Cells used for experiments exhibited a viability of >95%.

4.5. RT² Profiler PCR Array (PAHS-026ZF)—Human Osteogenesis

The RT² Profiler PCR Array “Human Osteogenesis” (Qiagen, Hilden, Germany) covers 84 genes involved in osteogenesis. The arrays were performed according to the manufacturer´s instructions. Human osteoblast-like MG63 cells were incubated without stimulus (control), and with CoCl₂ (250 µM), CrCl₃ (250 µM), and CoCr-particles (1 × 10⁶/well) in 12-well plates for 24 h. This experimental design was repeated in 4 independent experiments. RNA was extracted using the RNeasy kit with an on-column DNAse digestion (Qiagen). For cDNA synthesis with the RT² First Strand Kit (Qiagen) 1 µg of total RNA was used. Briefly, following a genomic elimination step of 5 min incubation with Buffer GE at 42 °C, the reverse transcription was performed for 15 min at 42 °C. For the PCR reaction the cDNA reaction volume of 20 µL was mixed with water, and the 2× RT² SYBR green mastermix. 25 µL of this mixture were consistently pipetted into all wells of the 96-well preloaded PCR plate. The PCR was performed on a Roche Light Cycler 480 (software 1.5.0 SP4) (Roche, Rotkreuz, Switzerland) according to protocol F of the manufacturer´s manual.

4.6. Cell Migration Assay

For the cell migration assays, 2-well silicone inserts with a defined free gap (ibidi^®, Martinsried, Germany) were placed on 12-well cell culture plates. 70 µL of a cell suspension, containing 3 × 10⁵ MG63 or SaOs-2 cells/mL, were filled into the chambers and incubated overnight. After removal of the inserts, a cell free gap of 500 µm was visible under the light microscope. After addition of Co²⁺, Cr³⁺, and CoCr particles, the closure of the gap was analyzed over a period of 48 h.

4.7. Detection of Proliferation

The proliferation of MG63 and SaO-2 cells was measured using the BrdU cell Proliferation Assay Kit (Cell Signaling Technology, Frankfurt, Germany) according to the manufacturer´s instructions. This kit detects 5-bromo-2′-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. Briefly, 5 × 10³ cells were seeded into 96-well cell culture plates and stimulated with CoCl₂ and CrCl₃ (0–250 µM) for 48 h. The fixed and permeabilized cells were subsequently incubated with the anti-BrdU antibody and then with the horseradish peroxidase (HRP)-conjugated secondary antibody. Incubation with 3,3′,5,5′-tetramethylbenzidine (TMB) stained the cells blue—it’s intensity correlates with BrdU incorporation. After 30 min the reaction was stopped by adding sulphuric acid. The absorbance was measured at 450 nm using a Tecan Infinite F200 Pro multiplate reader (Tecan, Männedorf, Switzerland).

4.8. Detection of Collagen Secretion

For determination of collagen secretion, 3 × 10⁵ cells per well in a 12-well plate were incubated for 48 h with CoCl₂ and CrCl₃, respectively. Then the cells were washed three times with PBS, fixed for 30 min with formalin and washed three times with destilled water. 400 µL of Sirius Red staining solution (0.1% direct red 80 (Sigma, Taufkirchen, Germany) in water-saturated picric acid) was added to each well and incubated for 30 min. Unbound dye was removed by repeated washing with 2.5% acetic acid. To quantify collagen staining the dye was extracted with 0.1 N NaOH and the absorption was measured at 540 nm.

4.9. Statistics

The statistical analysis was performed using SPSS 24. If not stated otherwise data were expressed as mean ± standard deviation (SD). Data from the stimulation experiments were analyzed using the one-way analysis of Variance (ANOVA) with different metal concentrations or with different time points as independent factors, and with post hoc Bonferroni test for pairwise analysis.

4.10. Analysis of Array Data

For analysis of RT² Profiler PCR array data, a web-based software (available online: https://www.qiagen.com/de/shop/genes-and-pathways/data-analysis-center) was used.

The ΔΔC_t method was used to calculate fold changes between treated groups and control group in which delta C_t is calculated between gene of interest and GAPDH as a housekeeper. The statistical significance of differences among the treated groups vs. untreated control was examined by Student’s t test. As a result of multiple testing the p value for significance was adjusted to 0.0006.