Next Article in Journal
Regulation of iNOS on Immune Cells and Its Role in Diseases
Next Article in Special Issue
New Insight into Sperm Capacitation: A Novel Mechanism of 17β-Estradiol Signalling
Previous Article in Journal
Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
Previous Article in Special Issue
Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa
Article

Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice

1
The Shraga Segal Department of Microbiology, Immunology, and Genetics, Beer-Sheva 84105, Israel
2
The Center of Advanced Research and Education in Reproduction (CARER), Faculty of Health Sciences, Beer-Sheva 8410501, Israel
3
Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel
4
Department of Pediatric Oncology and Department of Hematology, Soroka University Medical Center, Beer-Sheva 8410501, Israel
5
Molecular and Cell Genetics Laboratory, The CAS Key Laboratory of Innate Immunity and Chronic Disease, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230000, China
6
Institute of Human Genetics, Saarland University, Homburg/Saar, 66421 Homburg, Germany
7
Fertility and IVF Unit and Department of Obstetrics and Gynecology, Soroka University Medical Center, Beer-Sheva 8410501, Israel
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(12), 3804; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123804
Received: 5 November 2018 / Revised: 20 November 2018 / Accepted: 24 November 2018 / Published: 29 November 2018
(This article belongs to the Special Issue Molecular Biology of Spermatozoa)
Aggressive chemotherapy may lead to permanent male infertility. Prepubertal males do not generate sperm, but their testes do contain spermatogonial cells (SPGCs) that could be used for fertility preservation. In the present study, we examined the effect of busulfan (BU) on the SPGCs of immature mice, and the possible induction of the survivor SPGCs to develop spermatogenesis in 3D in-vitro culture. Immature mice were injected with BU, and after 0.5–12 weeks, their testes were weighed and evaluated histologically compared to the control mice. The spermatogonial cells [Sal-like protein 4 (SALL4) and VASA (a member of the DEAD box protein family) in the testicular tissue were counted/seminiferous tubule (ST). The cells from the STs were enzymatically isolated and cultured in vitro. Our results showed a significant decrease in the testicular weight of the BU-treated mice compared to the control. This was in parallel to a significant increase in the number of severely damaged STs, and a decrease in the number of SALL4 and VASA/STs compared to the control. The cultures of the isolated cells from the STs of the BU-treated mice showed a development of colonies and meiotic and post-meiotic cells after four weeks of culture. The addition of homogenates from adult GFP mice to those cultures induced the development of sperm-like cells after four weeks of culture. This is the first study demonstrating the presence of biologically active spermatogonial cells in the testicular tissue of BU-treated immature mice, and their capacity to develop sperm-like cells in vitro. View Full-Text
Keywords: chemotherapy; male infertility; spermatogenesis; testis; three-dimensional culture; spermatogonial cells chemotherapy; male infertility; spermatogenesis; testis; three-dimensional culture; spermatogonial cells
Show Figures

Figure 1

MDPI and ACS Style

AbuMadighem, A.; Solomon, R.; Stepanovsky, A.; Kapelushnik, J.; Shi, Q.; Meese, E.; Lunenfeld, E.; Huleihel, M. Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice. Int. J. Mol. Sci. 2018, 19, 3804. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123804

AMA Style

AbuMadighem A, Solomon R, Stepanovsky A, Kapelushnik J, Shi Q, Meese E, Lunenfeld E, Huleihel M. Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice. International Journal of Molecular Sciences. 2018; 19(12):3804. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123804

Chicago/Turabian Style

AbuMadighem, Ali, Ronnie Solomon, Alina Stepanovsky, Joseph Kapelushnik, QingHua Shi, Eckart Meese, Eitan Lunenfeld, and Mahmoud Huleihel. 2018. "Development of Spermatogenesis In Vitro in Three-Dimensional Culture from Spermatogonial Cells of Busulfan-Treated Immature Mice" International Journal of Molecular Sciences 19, no. 12: 3804. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123804

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop