Next Article in Journal
The Human Microbiota and Obesity: A Literature Systematic Review of In Vivo Models and Technical Approaches
Previous Article in Journal
The Role of Hydrogen Bonding in the Folding/Unfolding Process of Hydrated Lysozyme: A Review of Recent NMR and FTIR Results
Article

Expression Profile of Diabetes-Related Genes Associated with Leukocyte Sirtuin 1 Overexpression in Gestational Diabetes

1
Department of Structural Biology, Faculty of Biomedical Sciences and Postgraduate Education, Medical University of Lodz, 90-752 Lodz, Poland
2
Diabetology and Metabolic Diseases Department, Medical University of Lodz, 92-213 Lodz
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(12), 3826; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123826
Received: 29 September 2018 / Revised: 26 November 2018 / Accepted: 28 November 2018 / Published: 30 November 2018
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Although compelling evidence indicates that Sirtuin 1 (SIRT1) plays a prominent role in type 2 diabetes, its relationship with gestational diabetes (GDM) remains elusive. This study was aimed at identifying diabetes-related genes and cellular pathways linked to changes of leukocyte SIRT1 expression at the time of GDM diagnosis. For this purpose, 122 GDM patients were screened for leukocyte SIRT1 expression, and two subgroups were distinguished, namely GDM/SIRT1(↑) (n = 30, p < 0.05) and GDM/SIRT1(↔) (n = 92, p > 0.05), with significant and insignificant changes in leukocyte SIRT1 expression compared to a normal glucose tolerant (NGT) group (n = 41), respectively. PCR array analysis identified 11 diabetes-related genes with at least a ± 2-fold difference in expression in GDM/SIRT1(↑) patients (n = 9) vs. NGT controls (n = 7); in addition, significant differences in the expression of four of the six investigated genes were confirmed between the entire GDM/SIRT1(↑) group and the whole NGT group (p < 0.05). Interestingly, of these four genes, only ACLY expression was found to significantly differ between GDM/SIRT1(↑) and GDM/SIRT1(↔). This study demonstrates that under hyperglycemic conditions, leukocyte SIRT1 overexpression is accompanied by an over-abundance of three transcripts and an under-abundance of another; these four govern related metabolism, inflammation, and transport functions, suggesting that such alterations might represent systemic biological adaptations with a unique ACLY under-expression in GDM/SIRT1(↑) women. View Full-Text
Keywords: gene expression; gestational diabetes mellitus (GDM); Ingenuity Pathway Analysis (IPA); PCR array; Sirtuin 1 (SIRT1) gene expression; gestational diabetes mellitus (GDM); Ingenuity Pathway Analysis (IPA); PCR array; Sirtuin 1 (SIRT1)
Show Figures

Graphical abstract

MDPI and ACS Style

Mac-Marcjanek, K.; Zieleniak, A.; Zurawska-Klis, M.; Cypryk, K.; Wozniak, L.; Wojcik, M. Expression Profile of Diabetes-Related Genes Associated with Leukocyte Sirtuin 1 Overexpression in Gestational Diabetes. Int. J. Mol. Sci. 2018, 19, 3826. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123826

AMA Style

Mac-Marcjanek K, Zieleniak A, Zurawska-Klis M, Cypryk K, Wozniak L, Wojcik M. Expression Profile of Diabetes-Related Genes Associated with Leukocyte Sirtuin 1 Overexpression in Gestational Diabetes. International Journal of Molecular Sciences. 2018; 19(12):3826. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123826

Chicago/Turabian Style

Mac-Marcjanek, Katarzyna, Andrzej Zieleniak, Monika Zurawska-Klis, Katarzyna Cypryk, Lucyna Wozniak, and Marzena Wojcik. 2018. "Expression Profile of Diabetes-Related Genes Associated with Leukocyte Sirtuin 1 Overexpression in Gestational Diabetes" International Journal of Molecular Sciences 19, no. 12: 3826. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19123826

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop