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Open AccessArticle

Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression

1
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City 305-8572, Ibaraki, Japan
2
Tsukuba Life Science Innovation Program (T-LSI), University of Tsukuba, Tennodai 1-1-1, Tsukuba City 305-8577, Ibaraki, Japan
3
Interdisciplinary Research Center for Catalytic Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8560, Japan
4
Faculty of Sciences Semlalia, Cadi Ayyad University, Avenue Prince MoulayAbdellah, BP 2390, 40000 Marrakesh, Morocco
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3556; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20143556
Received: 24 June 2019 / Revised: 16 July 2019 / Accepted: 18 July 2019 / Published: 20 July 2019
(This article belongs to the Special Issue Natural Products and Neuroprotection)
Lippia citriodora ethanolic extract (VEE) and verbascoside (Vs), a phenypropanoid glycoside, have been demonstrated to exert relaxant and anxiolytic properties. However, the molecular mechanisms behind their effects are still unclear. In this work, we studied the effects and action mechanisms of VEE and Vs in vivo and in vitro, on human neurotypic SH-SY5Y cells.TST was conducted on mice treated orally with VEE (25, 50 and 100 mg/Kg), Vs (2.5 and 5 mg/Kg), Bupropion (20 mg/Kg) and Milli-Q water. Higher dose of VEE-treated mice showed an increase of immobility time compared to control groups, indicating an induction of relaxation. This effect was found to be induced by regulation of genes playing key roles in calcium homeostasis (calcium channels), cyclic AMP (cAMP) production and energy metabolism. On the other hand, low doses of VEE and Vs showed an antidepressant-like effect and was confirmed by serotonin, noradrenalin, dopamine and BDNF expressions. Finally, VEE and Vsenhancedcell viability, mitochondrial activity and calcium uptake in vitro confirming in vivo findings. Our results showed induction of relaxation and antidepressant-like effects depending on the administered dose of VEE and Vs, through modulation of cAMP and calcium. View Full-Text
Keywords: Lippia citriodora; VEE; Vs; relaxation; depression; mitochondria; cyclic AMP; calcium Lippia citriodora; VEE; Vs; relaxation; depression; mitochondria; cyclic AMP; calcium
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MDPI and ACS Style

Sabti, M.; Sasaki, K.; Gadhi, C.; Isoda, H. Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression. Int. J. Mol. Sci. 2019, 20, 3556. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20143556

AMA Style

Sabti M, Sasaki K, Gadhi C, Isoda H. Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression. International Journal of Molecular Sciences. 2019; 20(14):3556. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20143556

Chicago/Turabian Style

Sabti, Mouad; Sasaki, Kazunori; Gadhi, Chemseddoha; Isoda, Hiroko. 2019. "Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression" Int. J. Mol. Sci. 20, no. 14: 3556. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20143556

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