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Article

Ocular-Component-Specific miRNA Expression in a Murine Model of Lens-Induced Myopia

1
Department of Ophthalmology, School of Medicine, Keio University, Shinjuku-ku, Tokyo 160-8582, Japan
2
Laboratory of Photobiology, School of Medicine, Keio University, Shinjuku-ku, Tokyo 160-8582, Japan
3
Santen Pharmaceutical Co., Ltd., Osaka 530-8582, Japan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(15), 3629; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20153629
Received: 31 May 2019 / Revised: 14 July 2019 / Accepted: 22 July 2019 / Published: 24 July 2019
To identify tissues and molecules involved in refractive myopic shift and axial length elongation in a murine lens-induced myopia model, we performed a comprehensive analysis of microRNA (miRNA) expression. Three weeks after negative 30 diopter lens fixation on three-week-old C57BL/6J mice, total RNA was extracted from individual ocular components including cornea, iris, lens, retina, retinal pigment epithelium (RPE)/choroid, and sclera tissue. The miRNA expression analysis was pooled from three samples and carried out using Agilent Mouse miRNA Microarray (8 × 60 K) miRBase21.0. The expression ratio was calculated, and differentially expressed miRNAs were extracted, using GeneSpring GX 14.5. Myopic induction showed a significant myopic refractive change, axial elongation, and choroidal thinning. Through the comprehensive miRNA analysis, several upregulated miRNAs (56 in cornea tissue, 13 in iris tissue, 6 in lens tissue, 0 in retina tissue, 29 in RPE/choroid tissue, and 30 in sclera tissue) and downregulated miRNAs (7 in cornea tissue, 28 in iris tissue, 17 in lens tissue, 9 in retina tissue, 7 in RPE/choroid tissue, and 40 in sclera tissue) were observed. Overlapping expression changes in miRNAs were also found in different ocular components. Some of this miRNA dysregulation may be functionally involved in refractive myopia shift and axial length elongation. View Full-Text
Keywords: miRNA; lens-induced myopia; ocular components miRNA; lens-induced myopia; ocular components
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MDPI and ACS Style

Tanaka, Y.; Kurihara, T.; Hagiwara, Y.; Ikeda, S.-i.; Mori, K.; Jiang, X.; Torii, H.; Tsubota, K. Ocular-Component-Specific miRNA Expression in a Murine Model of Lens-Induced Myopia. Int. J. Mol. Sci. 2019, 20, 3629. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20153629

AMA Style

Tanaka Y, Kurihara T, Hagiwara Y, Ikeda S-i, Mori K, Jiang X, Torii H, Tsubota K. Ocular-Component-Specific miRNA Expression in a Murine Model of Lens-Induced Myopia. International Journal of Molecular Sciences. 2019; 20(15):3629. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20153629

Chicago/Turabian Style

Tanaka, Yasuhisa; Kurihara, Toshihide; Hagiwara, Yumi; Ikeda, Shin-ichi; Mori, Kiwako; Jiang, Xiaoyan; Torii, Hidemasa; Tsubota, Kazuo. 2019. "Ocular-Component-Specific miRNA Expression in a Murine Model of Lens-Induced Myopia" Int. J. Mol. Sci. 20, no. 15: 3629. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20153629

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