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Open AccessArticle

Cdh4 Down-Regulation Impairs in Vivo Infiltration and Malignancy in Patients Derived Glioblastoma Cells

1
U.O. Oncologia Cellulare, Ospedale Policlinico San Martino, 16132 Genova, Italy
2
Department of experimental medicine (DIMES), University of Genova, 16132 Genova, Italy
3
U.O. Molecular Pathology, Ospedale Policlinico San Martino, 16132 Genova, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(16), 4028; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20164028
Received: 26 June 2019 / Revised: 13 August 2019 / Accepted: 15 August 2019 / Published: 18 August 2019
(This article belongs to the Special Issue Activations of Cadherin Signaling in Cancer)
The high invasive phenotype of glioblastoma is one of the main causes of therapy inefficacy and tumor relapse. Cell adhesion molecules of the cadherin family are involved in cell migration and are known as master regulators of epithelial tumor invasiveness, but their role in glioblastoma is less understood. In particular, we recently demonstrated, in the syngeneic murine model, the occurrence of a previously undescribed cadherin switch between Cdh2 and Cdh4 during gliomagenesis, which is necessary for the acquisition of the highly infiltrative and tumorigenic phenotype of these cells. In the present study, we tested the role of Cdh4 in human gliomas. Our results on patient-derived glioma cells demonstrate a positive correlation between Cdh4 expression levels and the loss of cell–cell contact inhibition of proliferation controls that allows cells to proliferate over confluence. Moreover, the silencing of Cdh4 by artificial microRNAs induced a decrease in the infiltrative ability of human glioma cells both in vitro and in vivo. More strikingly, Cdh4 silencing induced an impairment of the tumorigenic potential of these cells after orthotopic transplantation in immunodeficient mice. Overall, we conclude that in human glioblastoma, Cdh4 can also actively contribute in regulating cell invasiveness and malignancy. View Full-Text
Keywords: brain tumor; contact inhibition; R-cadherin; proliferation; migration; adherent junctions; cell polarity brain tumor; contact inhibition; R-cadherin; proliferation; migration; adherent junctions; cell polarity
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MDPI and ACS Style

Ceresa, D.; Alessandrini, F.; Bosio, L.; Marubbi, D.; Reverberi, D.; Malatesta, P.; Appolloni, I. Cdh4 Down-Regulation Impairs in Vivo Infiltration and Malignancy in Patients Derived Glioblastoma Cells. Int. J. Mol. Sci. 2019, 20, 4028. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20164028

AMA Style

Ceresa D, Alessandrini F, Bosio L, Marubbi D, Reverberi D, Malatesta P, Appolloni I. Cdh4 Down-Regulation Impairs in Vivo Infiltration and Malignancy in Patients Derived Glioblastoma Cells. International Journal of Molecular Sciences. 2019; 20(16):4028. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20164028

Chicago/Turabian Style

Ceresa, Davide; Alessandrini, Francesco; Bosio, Lorenzo; Marubbi, Daniela; Reverberi, Daniele; Malatesta, Paolo; Appolloni, Irene. 2019. "Cdh4 Down-Regulation Impairs in Vivo Infiltration and Malignancy in Patients Derived Glioblastoma Cells" Int. J. Mol. Sci. 20, no. 16: 4028. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20164028

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