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Article

Unravelling the Metabolic and Hormonal Machinery During Key Steps of Somatic Embryogenesis: A Case Study in Coffee

1
Nestlé Research—Plant Science Unit, 101 avenue Gustave Eiffel, F-37097 Tours CEDEX 2, France
2
CIRAD (Centre de coopération internationale en recherche agronomique pour le développement), UMR IPME, F-34398 Montpellier, France
3
UMR IPME (Interactions Plantes Microorganismes Environnement), University of Montpellier, CIRAD, IRD, F-34398 Montpellier, France
4
IRD (Institut de recherche pour le développement), UMR IPME, F-34398 Montpellier, France
5
Max Planck Institute for Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Golm, Germany
6
Université de Lyon (Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR1418), F-69622 Lyon, France
7
Histocytology and Plant Cell Imaging platform PHIV, UMR AGAP (CIRAD, INRA, SupAgro)-UMR B&PMP (INRA, CNRS, SupAgro, University of Montpellier), F-34095 Montpellier, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(19), 4665; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194665
Received: 20 August 2019 / Revised: 17 September 2019 / Accepted: 18 September 2019 / Published: 20 September 2019
(This article belongs to the Special Issue Plant Cell and Organism Development)
Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination of elite varieties. However, for many plant species, optimizing SE protocols still relies on a trial-and-error approach. Using coffee as a model plant, we report here the first global analysis of metabolome and hormone dynamics aiming to unravel mechanisms regulating cell fate and totipotency. Sampling from leaf explant dedifferentiation until embryo development covered 15 key stages. An in-depth statistical analysis performed on 104 metabolites revealed that massive re-configuration of metabolic pathways induced SE. During initial dedifferentiation, a sharp decrease in phenolic compounds and caffeine levels was also observed while auxins, cytokinins and ethylene levels were at their highest. Totipotency reached its highest expression during the callus stages when a shut-off in hormonal and metabolic pathways related to sugar and energetic substance hydrolysis was evidenced. Abscisic acid, leucine, maltotriose, myo-inositol, proline, tricarboxylic acid cycle metabolites and zeatin appeared as key metabolic markers of the embryogenic capacity. Combining metabolomics with multiphoton microscopy led to the identification of chlorogenic acids as markers of embryo redifferentiation. The present analysis shows that metabolite fingerprints are signatures of cell fate and represent a starting point for optimizing SE protocols in a rational way. View Full-Text
Keywords: cell imaging; cell fate; coffee; histology; hormone content; metabolomics; somatic embryogenesis; totipotency cell imaging; cell fate; coffee; histology; hormone content; metabolomics; somatic embryogenesis; totipotency
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MDPI and ACS Style

Awada, R.; Campa, C.; Gibault, E.; Déchamp, E.; Georget, F.; Lepelley, M.; Abdallah, C.; Erban, A.; Martinez-Seidel, F.; Kopka, J.; Legendre, L.; Léran, S.; Conéjéro, G.; Verdeil, J.-L.; Crouzillat, D.; Breton, D.; Bertrand, B.; Etienne, H. Unravelling the Metabolic and Hormonal Machinery During Key Steps of Somatic Embryogenesis: A Case Study in Coffee. Int. J. Mol. Sci. 2019, 20, 4665. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194665

AMA Style

Awada R, Campa C, Gibault E, Déchamp E, Georget F, Lepelley M, Abdallah C, Erban A, Martinez-Seidel F, Kopka J, Legendre L, Léran S, Conéjéro G, Verdeil J-L, Crouzillat D, Breton D, Bertrand B, Etienne H. Unravelling the Metabolic and Hormonal Machinery During Key Steps of Somatic Embryogenesis: A Case Study in Coffee. International Journal of Molecular Sciences. 2019; 20(19):4665. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194665

Chicago/Turabian Style

Awada, Rayan; Campa, Claudine; Gibault, Estelle; Déchamp, Eveline; Georget, Frédéric; Lepelley, Maud; Abdallah, Cécile; Erban, Alexander; Martinez-Seidel, Federico; Kopka, Joachim; Legendre, Laurent; Léran, Sophie; Conéjéro, Geneviève; Verdeil, Jean-Luc; Crouzillat, Dominique; Breton, David; Bertrand, Benoît; Etienne, Hervé. 2019. "Unravelling the Metabolic and Hormonal Machinery During Key Steps of Somatic Embryogenesis: A Case Study in Coffee" Int. J. Mol. Sci. 20, no. 19: 4665. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194665

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