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Phytohormones (Auxin, Gibberellin) and ACC Deaminase In Vitro Synthesized by the Mycoparasitic Trichoderma DEMTkZ3A0 Strain and Changes in the Level of Auxin and Plant Resistance Markers in Wheat Seedlings Inoculated with this Strain Conidia

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Department of Environmental Microbiology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
2
Military Institute of Hygiene and Epidemiology, Lubelska St. 2, 24-100 Puławy, Poland
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Department of Plant Physiology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
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ŁUKASIEWICZ Research Network—New Chemical Syntheses Institute, Tysiąclecia Państwa Polskiego Ave. 13a, 24-110 Puławy, Poland
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Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(19), 4923; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194923
Received: 31 August 2019 / Revised: 25 September 2019 / Accepted: 1 October 2019 / Published: 4 October 2019
Both hormonal balance and plant growth may be shaped by microorganisms synthesizing phytohormones, regulating its synthesis in the plant and inducing plant resistance by releasing elicitors from cell walls (CW) by degrading enzymes (CWDE). It was shown that the Trichoderma DEMTkZ3A0 strain, isolated from a healthy rye rhizosphere, colonized the rhizoplane of wheat seedlings and root border cells (RBC) and caused approximately 40% increase of stem weight. The strain inhibited (in over 90%) the growth of polyphagous Fusarium spp. (F. culmorum, F. oxysporum, F. graminearum) phytopathogens through a mechanism of mycoparasitism. Chitinolytic and glucanolytic activity, strongly stimulated by CW of F. culmorum in the DEMTkZ3A0 liquid culture, is most likely responsible for the lysis of hyphae and macroconidia of phytopathogenic Fusarium spp. as well as the release of plant resistance elicitors. In DEMTkZ3A0 inoculated plants, an increase in the activity of the six tested plant resistance markers and a decrease in the concentration of indoleacetic acid (IAA) auxin were noted. IAA and gibberellic acid (GA) but also the 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD) enzyme regulating ethylene production by plant were synthesized by DEMTkZ3A0 in the liquid culture. IAA synthesis was dependent on tryptophan and negatively correlated with temperature, whereas GA synthesis was positively correlated with the biomass and temperature. View Full-Text
Keywords: Trichoderma; auxin indoleacetic acid (IAA); gibberellins; 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase; plant resistance markers; mycoparasitism Trichoderma; auxin indoleacetic acid (IAA); gibberellins; 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase; plant resistance markers; mycoparasitism
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MDPI and ACS Style

Jaroszuk-Ściseł, J.; Tyśkiewicz, R.; Nowak, A.; Ozimek, E.; Majewska, M.; Hanaka, A.; Tyśkiewicz, K.; Pawlik, A.; Janusz, G. Phytohormones (Auxin, Gibberellin) and ACC Deaminase In Vitro Synthesized by the Mycoparasitic Trichoderma DEMTkZ3A0 Strain and Changes in the Level of Auxin and Plant Resistance Markers in Wheat Seedlings Inoculated with this Strain Conidia. Int. J. Mol. Sci. 2019, 20, 4923. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194923

AMA Style

Jaroszuk-Ściseł J, Tyśkiewicz R, Nowak A, Ozimek E, Majewska M, Hanaka A, Tyśkiewicz K, Pawlik A, Janusz G. Phytohormones (Auxin, Gibberellin) and ACC Deaminase In Vitro Synthesized by the Mycoparasitic Trichoderma DEMTkZ3A0 Strain and Changes in the Level of Auxin and Plant Resistance Markers in Wheat Seedlings Inoculated with this Strain Conidia. International Journal of Molecular Sciences. 2019; 20(19):4923. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194923

Chicago/Turabian Style

Jaroszuk-Ściseł, Jolanta, Renata Tyśkiewicz, Artur Nowak, Ewa Ozimek, Małgorzata Majewska, Agnieszka Hanaka, Katarzyna Tyśkiewicz, Anna Pawlik, and Grzegorz Janusz. 2019. "Phytohormones (Auxin, Gibberellin) and ACC Deaminase In Vitro Synthesized by the Mycoparasitic Trichoderma DEMTkZ3A0 Strain and Changes in the Level of Auxin and Plant Resistance Markers in Wheat Seedlings Inoculated with this Strain Conidia" International Journal of Molecular Sciences 20, no. 19: 4923. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20194923

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