MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis
Abstract
:1. Introduction
2. Results
2.1. miR-29 Overexpression Compromised Glucocorticoid-Induced Bone Loss
2.2. miR-29 Repressed the Glucocorticoid-Induced Osteoclastic Erosion Histopathology
2.3. miR-29a Inhibited Osteoclast Differentiation and Resorption Capacity
2.4. TNFSF13b-Mediated miR-29a Regulation of Osoteoclast Formation
2.5. SOCS2 Controlled the miR-29a Inhibition of TNFSF13b Signaling
3. Discussion
4. Materials and Methods
4.1. miR-29a Transgenic Mice
4.2. Glucocorticoid-Induced Osteoporosis
4.3. Quantification of Serum Bone Resorption Markers
4.4. Assay of Bone Mass and Microstructure
4.5. In Situ Hybridization and Histomorphometry
4.6. Ex Vivo Osteoclast Differentiation and F-Actin Ring Immunofluorescence Labeling
4.7. Pit Formation
4.8. Quantitative RT-PCR
4.9. Immunoblotting
4.10. Chromatin Immuneprecipitation (ChIP)-PCR
4.11. Transfection
4.12. Statistical Analysis
Author Contributions
Finding
Acknowledgments
Conflicts of Interest
References
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Wu, R.-W.; Lian, W.-S.; Chen, Y.-S.; Kuo, C.-W.; Ke, H.-C.; Hsieh, C.-K.; Wang, S.-Y.; Ko, J.-Y.; Wang, F.-S. MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis. Int. J. Mol. Sci. 2019, 20, 5141. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205141
Wu R-W, Lian W-S, Chen Y-S, Kuo C-W, Ke H-C, Hsieh C-K, Wang S-Y, Ko J-Y, Wang F-S. MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis. International Journal of Molecular Sciences. 2019; 20(20):5141. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205141
Chicago/Turabian StyleWu, Re-Wen, Wei-Shiung Lian, Yu-Shan Chen, Chung-Wen Kuo, Huei-Ching Ke, Chin-Kuei Hsieh, Shao-Yu Wang, Jih-Yang Ko, and Feng-Sheng Wang. 2019. "MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis" International Journal of Molecular Sciences 20, no. 20: 5141. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205141