Protective Effects of Flavone from Tamarix aphylla against CCl4-Induced Liver Injury in Mice Mediated by Suppression of Oxidative Stress, Apoptosis and Angiogenesis
Abstract
:1. Introduction
2. Results
2.1. Chemical Elucidation of the Flavone
2.2. Histopathological Analysis of the Liver Tissues
2.3. Markers of Oxidative Stress in Liver Tissue Homogenates
2.4. Apoptosis Regulators in Liver Tissue
2.5. TGF-β1 Protein Levels in the Liver Tissue
2.6. CD31 Protein Levels in the Liver Tissue
3. Discussion
4. Materials and Methods
4.1. Extraction, Isolation and Elucidation of Flavone
4.2. Animals
4.3. Liver Injury Induction and Experimental Design
4.4. Histological Examination
4.5. Estimation of Malondialdehyde (MDA) Level in Liver Homogenates
4.6. Determination of Anti-Oxidant Enzymes Levels in Liver Homogenates
4.7. Immunohistochemical Examination of Apoptosis and Angiogenesis-Related Protein Markers
4.8. Statistical Analysis
5. Conclusions, Limitations and Future Research
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mice Group | SOD (U/g Tissue) | CAT (U/g Tissue) | Gpx (U/g Tissue) |
---|---|---|---|
Normal control | 1.23 ± 0.03 | 2.69 ± 0.01 | 38.74 ± 3.61 |
CCl4 model group | 0.32 ± 0.07 ### | 1.98 ± 0.23 ### | 21.76 ± 4.01 ## |
Flavone (10 mg/kg) | 0.58 ± 0.02* | 2.27 ± 0.03** | 27.65 ± 2.35* |
Flavone (25 mg/kg) | 0.89 ± 0.07** | 2.48 ± 0.29** | 30 ± 1.63* |
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El-Aarag, B.; Khairy, A.; Khalifa, S.A.M.; El-Seedi, H.R. Protective Effects of Flavone from Tamarix aphylla against CCl4-Induced Liver Injury in Mice Mediated by Suppression of Oxidative Stress, Apoptosis and Angiogenesis. Int. J. Mol. Sci. 2019, 20, 5215. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205215
El-Aarag B, Khairy A, Khalifa SAM, El-Seedi HR. Protective Effects of Flavone from Tamarix aphylla against CCl4-Induced Liver Injury in Mice Mediated by Suppression of Oxidative Stress, Apoptosis and Angiogenesis. International Journal of Molecular Sciences. 2019; 20(20):5215. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205215
Chicago/Turabian StyleEl-Aarag, Bishoy, Asmaa Khairy, Shaden A. M. Khalifa, and Hesham R. El-Seedi. 2019. "Protective Effects of Flavone from Tamarix aphylla against CCl4-Induced Liver Injury in Mice Mediated by Suppression of Oxidative Stress, Apoptosis and Angiogenesis" International Journal of Molecular Sciences 20, no. 20: 5215. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205215