Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice
Abstract
:1. Introduction
2. Results
2.1. Characterization of ONAC096
2.2. ONAC096 is Upregulated During Leaf Senescence
2.3. The onac096 Mutation Delays Leaf Yellowing during Natural and Dark-Induced Senescence
2.4. Mutation of ONAC096 Improves Grain Yield by Increasing the Number of Panicles Per Plant
2.5. ONAC096 Upregulates CDG and SAG Expression During Leaf Senescence
2.6. ONAC096 Mediates ABA-Induced Leaf Senescence
3. Discussion
3.1. Effects of ONAC096 on Leaf Senescence and Tillering
3.2. Involvement of ONAC096 in ABA-Induced Leaf Senescence
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Experimental Treatments
4.2. Determination of Photosynthetic Activity, Total Chlorophyll Content, and SPAD Value
4.3. RT-qPCR Analysis
4.4. Plasmid Construction and Rice Transformation
4.5. SDS-PAGE and Immunoblot Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
WT | wild type |
NLD | natural long day |
LD | long day |
CDGs | chlorophyll degradation genes |
SAGs | senescence-associated genes |
DIS | dark-induced senescence |
DDI | day(s) of dark incubation |
DAS | day(s) after seeding |
DAH | day(s) after heading |
DT | day(s) after treatment |
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Kang, K.; Shim, Y.; Gi, E.; An, G.; Paek, N.-C. Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice. Int. J. Mol. Sci. 2019, 20, 5241. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205241
Kang K, Shim Y, Gi E, An G, Paek N-C. Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice. International Journal of Molecular Sciences. 2019; 20(20):5241. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205241
Chicago/Turabian StyleKang, Kiyoon, Yejin Shim, Eunji Gi, Gynheung An, and Nam-Chon Paek. 2019. "Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice" International Journal of Molecular Sciences 20, no. 20: 5241. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205241