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Article

The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird

1
Structural Biology Laboratory, Department of Chemistry, The University of York, York YO10 5DD, UK
2
Novozymes A/S, Biologiens Vej 2, 2800 Kongens Lyngby, Denmark
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Current address: School of Life Sciences, Joseph Banks Laboratories, University of Lincoln, Green Lane, Lincoln, LN6 7DL, UK.
§
Current address: Novo Nordisk A/S, 2760 Måløv, Denmark.
Current address: AstraZeneca AB R & D, Pepparedsleden 1, 431 50 Mölndal, Sweden.
Int. J. Mol. Sci. 2019, 20(22), 5531; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225531
Received: 21 October 2019 / Revised: 31 October 2019 / Accepted: 1 November 2019 / Published: 6 November 2019
Muramidases/lysozymes are important bio-molecules, which cleave the glycan backbone in the peptidoglycan polymer found in bacterial cell walls. The glycoside hydrolase (GH) family 22 C-type lysozyme, from the folivorous bird Opisthocomus hoazin (stinkbird), was expressed in Aspergillus oryzae, and a set of variants was produced. All variants were enzymatically active, including those designed to probe key differences between the Hoatzin enzyme and Hen Egg White lysozyme. Four variants showed improved thermostability at pH 4.7, compared to the wild type. The X-ray structure of the enzyme was determined in the apo form and in complex with chitin oligomers. Bioinformatic analysis of avian GH22 amino acid sequences showed that they separate out into three distinct subgroups (chicken-like birds, sea birds and other birds). The Hoatzin is found in the “other birds” group and we propose that this represents a new cluster of avian upper-gut enzymes. View Full-Text
Keywords: lysozyme; peptidoglycan cleavage; avian gut GH22; crystal structure lysozyme; peptidoglycan cleavage; avian gut GH22; crystal structure
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MDPI and ACS Style

Taylor, E.J.; Skjøt, M.; Skov, L.K.; Klausen, M.; De Maria, L.; Gippert, G.P.; Turkenburg, J.P.; Davies, G.J.; Wilson, K.S. The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird. Int. J. Mol. Sci. 2019, 20, 5531. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225531

AMA Style

Taylor EJ, Skjøt M, Skov LK, Klausen M, De Maria L, Gippert GP, Turkenburg JP, Davies GJ, Wilson KS. The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird. International Journal of Molecular Sciences. 2019; 20(22):5531. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225531

Chicago/Turabian Style

Taylor, Edward J., Michael Skjøt, Lars K. Skov, Mikkel Klausen, Leonardo De Maria, Garry P. Gippert, Johan P. Turkenburg, Gideon J. Davies, and Keith S. Wilson 2019. "The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird" International Journal of Molecular Sciences 20, no. 22: 5531. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20225531

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