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Article

Quality Screening of Incorrectly Folded Soluble Aggregates from Functional Recombinant Proteins

Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(4), 907; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040907
Received: 8 January 2019 / Revised: 1 February 2019 / Accepted: 16 February 2019 / Published: 19 February 2019
(This article belongs to the Collection Protein Folding)
Solubility is the prime criterion for determining the quality of recombinant proteins, yet it often fails to represent functional activity due to the involvement of non-functional, misfolded, soluble aggregates, which compromise the quality of recombinant proteins. However, guidelines for the quality assessment of soluble proteins have neither been proposed nor rigorously validated experimentally. Using the aggregation-prone enhanced green-fluorescent protein (EGFP) folding reporter system, we evaluated the folding status of recombinant proteins by employing the commonly used sonication and mild lysis of recombinant host cells. We showed that the differential screening of solubility and folding competence is crucial for improving the quality of recombinant proteins without sacrificing their yield. These results highlight the importance of screening out incorrectly folded soluble aggregates at the initial purification step to ensure the functional quality of recombinant proteins. View Full-Text
Keywords: recombinant protein; soluble aggregates; cell lysis; sonication; mild lysis recombinant protein; soluble aggregates; cell lysis; sonication; mild lysis
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MDPI and ACS Style

Kwon, S.B.; Yu, J.E.; Kim, J.; Oh, H.; Park, C.; Lee, J.; Seong, B.L. Quality Screening of Incorrectly Folded Soluble Aggregates from Functional Recombinant Proteins. Int. J. Mol. Sci. 2019, 20, 907. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040907

AMA Style

Kwon SB, Yu JE, Kim J, Oh H, Park C, Lee J, Seong BL. Quality Screening of Incorrectly Folded Soluble Aggregates from Functional Recombinant Proteins. International Journal of Molecular Sciences. 2019; 20(4):907. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040907

Chicago/Turabian Style

Kwon, Soon B., Ji E. Yu, Jihoon Kim, Hana Oh, Chan Park, Jinhee Lee, and Baik L. Seong 2019. "Quality Screening of Incorrectly Folded Soluble Aggregates from Functional Recombinant Proteins" International Journal of Molecular Sciences 20, no. 4: 907. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040907

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