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Article

Botulinum Neurotoxin-A Injected Intrastriatally into Hemiparkinsonian Rats Improves the Initiation Time for Left and Right Forelimbs in Both Forehand and Backhand Directions

1
Institute of Anatomy, Rostock University Medical Center, D-18057 Rostock, Germany
2
Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Macroscopic and Clinical Anatomy, Medical University of Graz, A-8010 Graz, Austria
3
Institute of Medical Genetics, Rostock University Medical Center, D-18057 Rostock, Germany
4
Centre of Transdisciplinary Neuroscience Rostock, D-18147 Rostock, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(4), 992; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040992
Received: 11 February 2019 / Revised: 19 February 2019 / Accepted: 21 February 2019 / Published: 25 February 2019
Forelimb stepping is a widely used test for the assessment of forelimb akinesia in hemiparkinsonian (hemi-PD) rats. The initiation time (IT) is considered the most sensitive parameter in the stepping test procedure. Here we propose a novel, reliable, and simple method for the measurement of IT of both forelimbs in both forehand and backhand directions in rats. Evaluating the same videos taken for quantifying adjusting steps, IT measurements were done without additional experiments. This is in contrast to the classical approach introduced by Olsson et al. (1995), in which separate experiments are necessary. We successfully applied our approach to hemi-PD rats intrastriatally treated with botulinum neurotoxin-A (BoNT-A). In naïve rats, an IT of about 0.62 s was found, and in right-sided hemi-PD rats the IT of the left forepaw increased to about 3.62 s. These hemi-PD rats showed, however, reduced ITs of the impaired left forepaws 1 month and the second time 7 months after induction of hemi-PD via the injection of 1 ng BoNT-A into the ipsilateral striatum, depending on post BoNT-A survival time. The method described offers the possibility of a precise and animal-friendly evaluation of IT in rats, including the beneficial effect of BoNT-A treatment in hemi-PD rats. View Full-Text
Keywords: botulinum neurotoxin-A; stepping test; initiation time; hemiparkinsonian rat; 6-OHDA; basal ganglia; striatum; correlation analysis botulinum neurotoxin-A; stepping test; initiation time; hemiparkinsonian rat; 6-OHDA; basal ganglia; striatum; correlation analysis
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MDPI and ACS Style

Antipova, V.; Holzmann, C.; Hawlitschka, A.; Wree, A. Botulinum Neurotoxin-A Injected Intrastriatally into Hemiparkinsonian Rats Improves the Initiation Time for Left and Right Forelimbs in Both Forehand and Backhand Directions. Int. J. Mol. Sci. 2019, 20, 992. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040992

AMA Style

Antipova V, Holzmann C, Hawlitschka A, Wree A. Botulinum Neurotoxin-A Injected Intrastriatally into Hemiparkinsonian Rats Improves the Initiation Time for Left and Right Forelimbs in Both Forehand and Backhand Directions. International Journal of Molecular Sciences. 2019; 20(4):992. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040992

Chicago/Turabian Style

Antipova, Veronica, Carsten Holzmann, Alexander Hawlitschka, and Andreas Wree. 2019. "Botulinum Neurotoxin-A Injected Intrastriatally into Hemiparkinsonian Rats Improves the Initiation Time for Left and Right Forelimbs in Both Forehand and Backhand Directions" International Journal of Molecular Sciences 20, no. 4: 992. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20040992

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