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Open AccessArticle

In Vitro Anti-proliferative Activity and Mechanism of Action of Anemone nemorosa

Department of Biochemistry and Microbiology, PO Box 77000, Nelson Mandela University, Port Elizabeth 6031, South Africa
Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, Bucharest 020956, Romania
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1217;
Received: 8 February 2019 / Revised: 6 March 2019 / Accepted: 7 March 2019 / Published: 11 March 2019
(This article belongs to the Section Molecular Oncology)
Anemone nemorosa is part of the Ranunculaceae genus Anemone (order Ranunculales) which comprises more than 150 species. Various parts of the plant have been used for the treatment of numerous medical conditions such as headaches, tertian agues, rheumatic gout, leprosy, lethargy, eye inflammation as well as malignant and corroding ulcers. The Anemone plants have been found to contain various medicinal compounds with anti-cancer, immunomodulatory, anti-inflammatory, anti-oxidant and anti-microbial activities. To date there has been no reported evidence of its use in the treatment of cancer. However, due to the reported abundance of saponins which usually exert anti-cancer activity via cell cycle arrest and the induction of apoptosis, we investigated the mode of cell death induced by an aqueous A. nemorosa extract by using HeLa cervical cancer cells. Cisplatin was used as a positive control. With a 50% inhibitory concentration (IC50) of 20.33 ± 2.480 µg/mL, treatment with A. nemorosa yielded a delay in the early mitosis phase of the cell cycle. Apoptosis was confirmed through fluorescent staining with annexin V-FITC. Apoptosis was more evident with A. nemorosa treatment compared to the positive control after 24 and 48 h. Tetramethylrhodamine ethyl ester staining showed a decrease in mitochondrial membrane potential at 24 and 48 h. The results obtained imply that A. nemorosa may have potential anti-proliferative properties. View Full-Text
Keywords: A. nemorosa; cytotoxicity; apoptosis; HeLa A. nemorosa; cytotoxicity; apoptosis; HeLa
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MDPI and ACS Style

Swanepoel, B.; Venables, L.; Olaru, O.T.; Nitulescu, G.M.; van de Venter, M. In Vitro Anti-proliferative Activity and Mechanism of Action of Anemone nemorosa. Int. J. Mol. Sci. 2019, 20, 1217.

AMA Style

Swanepoel B, Venables L, Olaru OT, Nitulescu GM, van de Venter M. In Vitro Anti-proliferative Activity and Mechanism of Action of Anemone nemorosa. International Journal of Molecular Sciences. 2019; 20(5):1217.

Chicago/Turabian Style

Swanepoel, Bresler; Venables, Luanne; Olaru, Octavian T.; Nitulescu, George M.; van de Venter, Maryna. 2019. "In Vitro Anti-proliferative Activity and Mechanism of Action of Anemone nemorosa" Int. J. Mol. Sci. 20, no. 5: 1217.

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