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Article

A Cotton (Gossypium hirsutum) Myo-Inositol-1-Phosphate Synthase (GhMIPS1D) Gene Promotes Root Cell Elongation in Arabidopsis

by 1,†, 1,†, 1,†, 1, 1, 1, 1,2,* and 1,*
1
College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China
2
Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(5), 1224; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20051224
Received: 20 January 2019 / Revised: 22 February 2019 / Accepted: 26 February 2019 / Published: 11 March 2019
Myo-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) plays important roles in plant growth and development, stress responses, and cellular signal transduction. MIPS genes were found preferably expressed during fiber cell initiation and early fast elongation in upland cotton (Gossypium hirsutum), however, current understanding of the function and regulatory mechanism of MIPS genes to involve in cotton fiber cell growth is limited. Here, by genome-wide analysis, we identified four GhMIPS genes anchoring onto four chromosomes in G. hirsutum and analyzed their phylogenetic relationship, evolutionary dynamics, gene structure and motif distribution, which indicates that MIPS genes are highly conserved from prokaryotes to green plants, with further exon-intron structure analysis showing more diverse in Brassicales plants. Of the four GhMIPS members, based on the significant accumulated expression of GhMIPS1D at the early stage of fiber fast elongating development, thereby, the GhMIPS1D was selected to investigate the function of participating in plant development and cell growth, with ectopic expression in the loss-of-function Arabidopsis mips1 mutants. The results showed that GhMIPS1D is a functional gene to fully compensate the abnormal phenotypes of the deformed cotyledon, dwarfed plants, increased inflorescence branches, and reduced primary root lengths in Arabidopsis mips1 mutants. Furthermore, shortened root cells were recovered and normal root cells were significantly promoted by ectopic expression of GhMIPS1D in Arabidopsis mips1 mutant and wild-type plants respectively. These results serve as a foundation for understanding the MIPS family genes in cotton, and suggest that GhMIPS1D may function as a positive regulator for plant cell elongation. View Full-Text
Keywords: MIPS; exon-intron structure diversity; Gossypium hirsutum; loss-of-function mutant; root cell elongation MIPS; exon-intron structure diversity; Gossypium hirsutum; loss-of-function mutant; root cell elongation
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MDPI and ACS Style

Ma, R.; Song, W.; Wang, F.; Cao, A.; Xie, S.; Chen, X.; Jin, X.; Li, H. A Cotton (Gossypium hirsutum) Myo-Inositol-1-Phosphate Synthase (GhMIPS1D) Gene Promotes Root Cell Elongation in Arabidopsis. Int. J. Mol. Sci. 2019, 20, 1224. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20051224

AMA Style

Ma R, Song W, Wang F, Cao A, Xie S, Chen X, Jin X, Li H. A Cotton (Gossypium hirsutum) Myo-Inositol-1-Phosphate Synthase (GhMIPS1D) Gene Promotes Root Cell Elongation in Arabidopsis. International Journal of Molecular Sciences. 2019; 20(5):1224. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20051224

Chicago/Turabian Style

Ma, Rendi, Wangyang Song, Fei Wang, Aiping Cao, Shuangquan Xie, Xifeng Chen, Xiang Jin, and Hongbin Li. 2019. "A Cotton (Gossypium hirsutum) Myo-Inositol-1-Phosphate Synthase (GhMIPS1D) Gene Promotes Root Cell Elongation in Arabidopsis" International Journal of Molecular Sciences 20, no. 5: 1224. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20051224

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