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Effect of Ultraviolet-C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L.

1
Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan
2
Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d’Orléans, 45067 Orléans CEDEX 2, France
3
Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Rajathevi, Bangkok 10400, Thailand
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Institut de Chimie Organique et Analytique (ICOA) UMR7311, Université d’Orléans-CNRS, 45067 Orléans CEDEX 2, France
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COSM’ACTIFS, Bioactifs et Cosmétiques, CNRS GDR3711, 45067 Orléans CEDEX 2, France
6
EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, 37000 Tours, France
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(7), 1787; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071787
Received: 21 March 2019 / Revised: 8 April 2019 / Accepted: 9 April 2019 / Published: 11 April 2019
(This article belongs to the Section Bioactives and Nutraceuticals)
Lepidium sativum L. is a rich source of polyphenols that have huge medicinal and pharmaceutical applications. In the current study, an effective abiotic elicitation strategy was designed for enhanced biosynthesis of polyphenols in callus culture of L. sativum. Callus was exposed to UV-C radiations for different time intervals and various concentrations of melatonin. Secondary metabolites were quantified by using high-performance liquid chromatography (HPLC). Results indicated the total secondary metabolite accumulation of nine quantified compounds was almost three fold higher (36.36 mg/g dry weight (DW)) in melatonin (20 μM) treated cultures, whereas, in response to UV-C (60 min), a 2.5 fold increase (32.33 mg/g DW) was recorded compared to control (13.94 mg/g DW). Metabolic profiling revealed the presence of three major phytochemicals, i.e., chlorogenic acid, kaemferol, and quercetin, in callus culture of L. sativum. Furthermore, antioxidant, antidiabetic, and enzymatic activities of callus cultures were significantly enhanced. Maximum antidiabetic activities (α-glucosidase: 57.84%; α-amylase: 62.66%) were recorded in melatonin (20 μM) treated callus cultures. Overall, melatonin proved to be an effect elicitor compared to UV-C and a positive correlation in these biological activities and phytochemical accumulation was observed. The present study provides a better comparison of both elicitors and their role in the initiation of physiological pathways for enhanced metabolites biosynthesis in vitro callus culture of L. sativum. View Full-Text
Keywords: polyphenols; elicitation; ultraviolet rays; secondary metabolites; antidiabetic; melatonin polyphenols; elicitation; ultraviolet rays; secondary metabolites; antidiabetic; melatonin
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MDPI and ACS Style

Ullah, M.A.; Tungmunnithum, D.; Garros, L.; Drouet, S.; Hano, C.; Abbasi, B.H. Effect of Ultraviolet-C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L. Int. J. Mol. Sci. 2019, 20, 1787. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071787

AMA Style

Ullah MA, Tungmunnithum D, Garros L, Drouet S, Hano C, Abbasi BH. Effect of Ultraviolet-C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L. International Journal of Molecular Sciences. 2019; 20(7):1787. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071787

Chicago/Turabian Style

Ullah, Muhammad A., Duangjai Tungmunnithum, Laurine Garros, Samantha Drouet, Christophe Hano, and Bilal H. Abbasi 2019. "Effect of Ultraviolet-C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L." International Journal of Molecular Sciences 20, no. 7: 1787. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071787

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