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Article

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

1
Center of Biomaterials, University of Havana, Havan 10400, Cuba
2
National Institute of Technology, Rio de Janeiro-RJ 20081-312, Brazil
3
CUCEI, University of Guadalajara, Jalisco 44430, Mexico
4
Experimental Trauma Surgery, Dept. Trauma Surgery, School of Medicine, Klinikum rechts der Isar, Technical University of Munich, 81675 Munich, Germany
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(7), 1790; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071790
Received: 25 February 2019 / Revised: 2 April 2019 / Accepted: 9 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Biomaterials for Musculoskeletal System)
Ceramic materials mimic the mineral composition of native bone and feature osteoconductive properties; they are therefore used to regenerate bone tissue. Much research focuses on increasing the porosity and pore interconnectivity of ceramic scaffolds to increase osteoconductivity, cell migration and cell-cell interaction. We aimed to fabricate biocompatible 3D-scaffolds featuring macro- and microporous calcium phosphates with high pore interconnection. Nanoparticles of hydroxyapatite (HA) and calcium deficient hydroxyapatite (CDHA) were synthesized by wet chemical precipitation. Scaffolds were produced from them by the replication polymeric foam technique. Solid content and sintering temperature were varied. Nanoparticles and scaffolds were characterized regarding morphology, chemical and mineral composition, porosity and mechanical properties. Biocompatibility, cell attachment and distribution were evaluated in vitro with human adipose mesenchymal stem cells. Scaffolds with total porosity of 71%–87%, pores in the range of 280–550 µm and connectivity density up to 43 mm−3 were obtained. Smaller pore sizes were obtained at higher sintering temperature. High solid content resulted in a decrease of total porosity but increased interconnectivity. Scaffolds 50HA/50β-TCP featured superior interconnectivity and mechanical properties. They were bioactive and biocompatible. High HA solid content (40 wt.%) in the HA pure scaffolds was negative for cell viability and proliferation, while in the 50HA/50β-TCP composite scaffolds it resulted more biocompatible. View Full-Text
Keywords: bone tissue engineering; scaffolds; hydroxyapatite; β-tricalcium phosphate; biphasic calcium phosphates bone tissue engineering; scaffolds; hydroxyapatite; β-tricalcium phosphate; biphasic calcium phosphates
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MDPI and ACS Style

Morejón, L.; Delgado, J.A.; Antunes Ribeiro, A.; Varella de Oliveira, M.; Mendizábal, E.; García, I.; Alfonso, A.; Poh, P.; van Griensven, M.; Balmayor, E.R. Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles. Int. J. Mol. Sci. 2019, 20, 1790. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071790

AMA Style

Morejón L, Delgado JA, Antunes Ribeiro A, Varella de Oliveira M, Mendizábal E, García I, Alfonso A, Poh P, van Griensven M, Balmayor ER. Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles. International Journal of Molecular Sciences. 2019; 20(7):1790. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071790

Chicago/Turabian Style

Morejón, Lizette, José A. Delgado, Alexandre Antunes Ribeiro, Marize Varella de Oliveira, Eduardo Mendizábal, Ibrahim García, Adrián Alfonso, Patrina Poh, Martijn van Griensven, and Elizabeth R. Balmayor 2019. "Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles" International Journal of Molecular Sciences 20, no. 7: 1790. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071790

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