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Article

Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa

1
Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, 30100 Murcia, Spain
2
Department of Biology, Faculty of Sciences, University of Girona, 17003 Girona, Spain
3
School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
4
Department of Clinical and Experimental Medicine, Faculty of Medicine and Health Sciences, Linköping University, SE-58185 Linköping, Sweden
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(7), 1791; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071791
Received: 26 March 2019 / Accepted: 10 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Molecular Biology of Spermatozoa)
Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed pig spermatozoa retrieved from the cauda epididymis and the ejaculate of the same boars, with clear differences in cryotolerance. Spermatozoa were collected from 10 healthy, sexually mature, and fertile boars, and cryopreserved using a standard 0.5 mL-straw protocol. Total and progressive motility, viability, and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) epididymal than ejaculated spermatozoa. Quantitative proteomics of fresh and FT spermatozoa were analyzed using a LC-ESI-MS/MS-based Sequential Window Acquisition of All Theoretical Spectra approach. Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. Differential protein–protein networks highlighted a set of proteins quantitatively altered in ejaculated spermatozoa, directly involved in mitochondrial functionality which would explain why ejaculated spermatozoa deteriorate during cryopreservation. View Full-Text
Keywords: epididymis; ejaculate; spermatozoa; cryopreservation; proteomics; porcine epididymis; ejaculate; spermatozoa; cryopreservation; proteomics; porcine
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MDPI and ACS Style

Perez-Patiño, C.; Barranco, I.; Li, J.; Padilla, L.; Martinez, E.A.; Rodriguez-Martinez, H.; Roca, J.; Parrilla, I. Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa. Int. J. Mol. Sci. 2019, 20, 1791. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071791

AMA Style

Perez-Patiño C, Barranco I, Li J, Padilla L, Martinez EA, Rodriguez-Martinez H, Roca J, Parrilla I. Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa. International Journal of Molecular Sciences. 2019; 20(7):1791. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071791

Chicago/Turabian Style

Perez-Patiño, Cristina, Isabel Barranco, Junwei Li, Lorena Padilla, Emilio A. Martinez, Heriberto Rodriguez-Martinez, Jordi Roca, and Inmaculada Parrilla. 2019. "Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa" International Journal of Molecular Sciences 20, no. 7: 1791. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20071791

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