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Article

Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide

1
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
2
United States Department of Agriculture, Agriculture Research Service (USDA-ARS), Animal Genomics and Improvement Laboratory, Beltsville, MD 20705, USA
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(9), 2130; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092130
Received: 15 March 2019 / Revised: 21 April 2019 / Accepted: 26 April 2019 / Published: 30 April 2019
Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome. In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions. The exercise stress disrupted the network composition and microbial co-occurrence patterns. Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community. Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome. Several modules correlated with physiological parameters were detected. For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and −0.75, respectively). A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker. Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering. View Full-Text
Keywords: 16S rRNA gene; chondroitin sulfate disaccharide; co-occurrence network; global network; microbial interactions; microbiome; modularity; superoxide dismutase 16S rRNA gene; chondroitin sulfate disaccharide; co-occurrence network; global network; microbial interactions; microbiome; modularity; superoxide dismutase
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MDPI and ACS Style

Liu, F.; Li, Z.; Wang, X.; Xue, C.; Tang, Q.; Li, R.W. Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide. Int. J. Mol. Sci. 2019, 20, 2130. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092130

AMA Style

Liu F, Li Z, Wang X, Xue C, Tang Q, Li RW. Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide. International Journal of Molecular Sciences. 2019; 20(9):2130. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092130

Chicago/Turabian Style

Liu, Fang, Zhaojie Li, Xiong Wang, Changhu Xue, Qingjuan Tang, and Robert W. Li 2019. "Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide" International Journal of Molecular Sciences 20, no. 9: 2130. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092130

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