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Article

Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism

by 1,2, 1,†, 1,2, 1,2,‡, 1 and 1,*
1
Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Present address: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Present address: GeneFirst Limited, Abingdon, Oxfordshire OX14 3DB, UK.
Int. J. Mol. Sci. 2019, 20(9), 2339; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092339
Received: 21 April 2019 / Revised: 7 May 2019 / Accepted: 9 May 2019 / Published: 11 May 2019
ADP-ribosylation factor-guanine nucleotide exchange factors (ARF-GEFs) act as key regulators of vesicle trafficking in all eukaryotes. In Arabidopsis, there are eight ARF-GEFs, including three members of the GBF1 subfamily and five members of the BIG subfamily. These ARF-GEFs have different subcellular localizations and regulate different trafficking pathways. Until now, the roles of these BIG-subfamily ARF-GEFs have not been fully revealed. Here, analysis of the BIGs expression patterns showed that BIG3 and BIG5 have similar expression patterns. big5-1 displayed a dwarf growth and big3-1 big5-1 double mutant showed more severe defects, indicating functional redundancy between BIG3 and BIG5. Moreover, both big5-1 and big3-1 big5-1 exhibited a reduced sensitivity to Brassinosteroid (BR) treatment. Brefeldin A (BFA)-induced BR receptor Brassinosteroid insensitive 1 (BRI1) aggregation was reduced in big5-1 mutant, indicating that the action of BIG5 is required for BRI1 recycling. Furthermore, BR-induced dephosphorylation of transcription factor BZR1 was decreased in big3-1 big5-1 double mutants. The introduction of the gain-of-function of BZR1 mutant BZR1-1D in big3-1 big5-1 mutants can partially rescue the big3-1 big5-1 growth defects. Our findings revealed that BIG5 functions redundantly with BIG3 in plant growth and gravitropism, and BIG5 participates in BR signal transduction pathway through regulating BRI1 trafficking. View Full-Text
Keywords: ARF protein; BIG family ARF-GEF; auxin; brassinosteroids; gravity response ARF protein; BIG family ARF-GEF; auxin; brassinosteroids; gravity response
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MDPI and ACS Style

Xue, S.; Zou, J.; Liu, Y.; Wang, M.; Zhang, C.; Le, J. Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism. Int. J. Mol. Sci. 2019, 20, 2339. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092339

AMA Style

Xue S, Zou J, Liu Y, Wang M, Zhang C, Le J. Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism. International Journal of Molecular Sciences. 2019; 20(9):2339. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092339

Chicago/Turabian Style

Xue, Shan, Junjie Zou, Yangfan Liu, Ming Wang, Chunxia Zhang, and Jie Le. 2019. "Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism" International Journal of Molecular Sciences 20, no. 9: 2339. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20092339

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