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Article

Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

1
Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic
2
Department of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences, Mendel University, 61300 Brno, Czech Republic
3
Department of Plant Origin Foodstuffs Hygiene and Technology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, 61242 Brno, Czech Republic
4
Bioscience Center, National Renewable Energy Laboratory, 16253 Denver West Parkway, Golden, CO 80401, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 283; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010283
Received: 27 November 2019 / Revised: 26 December 2019 / Accepted: 30 December 2019 / Published: 31 December 2019
(This article belongs to the Special Issue Thermophilic and Hyperthermophilic Microbes and Enzymes)
Background: In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modřice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Čejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition of microbial communities in these plants. View Full-Text
Keywords: renewable energy; biogas; Illumina sequencing; thermophilic microorganisms; anaerobic digesters renewable energy; biogas; Illumina sequencing; thermophilic microorganisms; anaerobic digesters
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MDPI and ACS Style

Kushkevych, I.; Cejnar, J.; Vítězová, M.; Vítěz, T.; Dordević, D.; Bomble, Y.J. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. Int. J. Mol. Sci. 2020, 21, 283. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010283

AMA Style

Kushkevych I, Cejnar J, Vítězová M, Vítěz T, Dordević D, Bomble YJ. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. International Journal of Molecular Sciences. 2020; 21(1):283. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010283

Chicago/Turabian Style

Kushkevych, Ivan, Jiří Cejnar, Monika Vítězová, Tomáš Vítěz, Dani Dordević, and Yannick J. Bomble 2020. "Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants" International Journal of Molecular Sciences 21, no. 1: 283. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010283

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